TY - JOUR AU - C., LOHOUES ESSIS ESMEL AU - NOEL D., TREBISSOU JONHSON AU - M., DION-LAINE AU - J., DECHI JEAN AU - L., N’DIN JEAN AU - M., CAMARA-CISSE PY - 2015/09/01 Y2 - 2024/03/28 TI - GENETIC POLYMORPHISM OF REVERSE TRANSCRIPTASE AND PROTEASE ASSOCIATED WITH THE ANTIRETROVIRAL (ARV) INEFFICIENCY IN PEOPLE LIVING WITH HUMAN IMMUNODEFICIENCY VIRUS TYPE-2 (HIV-2) IN ABIDJAN, CÔTE D’IVOIRE JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 7 IS - 9 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/7215 SP - 210-212 AB - <p><strong>Objectives</strong>:<strong>&nbsp;</strong>To analyse the genetic polymorphism of reverse transcriptase and protease genes in patients living with HIV-2, to search for possible mutations preventing the effectiveness of antiretroviral drugs (ARVs).</p><p><strong>Methods:&nbsp;</strong>RNA Viral was extracted after lysis of the virus particles and purified on filter columns (Qiagen). After their extraction, DNA fragments were amplified by the method of reverse transcription/polymerase chain reaction (RT/PCR) on one hand and by the PCR method enchase on the other hand. The study of genetic polymorphisms in reverse transcriptase and protease was performed after sequencing of amplicons.</p><p><strong>Results:&nbsp;</strong>A significant polymorphism was observed in positions involved in resistance to antiretrovirals. The most frequent was mutations M 46 I, M 36 I/V, V82I, L 89 I on protease and Y 181 I, Y 188 L, G 190A on reverse transcriptase.</p><p><strong>Conclusion</strong>:<strong>&nbsp;</strong>Polymorphic mutations were observed in the genes of the protease and reverse transcriptase and could be involved in the HIV-2 resistance to antiretrovirals (ARVs). It would be desirable to do the quantification of DNA provirus of HIV-2 in the other for a durable therapeutic monitoring of patients having HIV-2.</p> ER -