TY - JOUR AU - R., Seema Devi AU - Thoppil, John Ernest PY - 2016/02/01 Y2 - 2024/03/29 TI - CYTOTOXIC STUDIES AND PHYTOCHEMICAL ANALYSIS OF ORTHOSIPHON THYMIFLORUS (ROTH) SLEESEN JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 8 IS - 2 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/9870 SP - 249-255 AB - <p><strong>Objective: </strong>Plant-derived compounds with therapeutic effect are getting attention all over the world. Researchers are mainly targeting to ethnic and folk knowledge for the exploration of novel drug candidates.<em> Orthosiphon thymiflorus </em>is a medicinal, aromatic herb used in folk medicine and Ayurvedic preparations. Hence, it is important to evaluate the cytotoxic potential and chemical profile of the plant.</p><p><strong>Methods: </strong>Cytotoxicity was evaluated <em>in vitro</em> in DLA and EAC cell lines and <em>in vivo</em> by <em>Allium</em> chromosome aberration assay. Phytochemical screening of the extract was done by UV/Vis spectroscopy and FT-IR.</p><p><strong>Results: </strong>Plant extracts showed significant toxicity in cell lines in higher concentration (100 µg/ml and 200 µg/ml). Cytotoxicity <em>in vivo</em> was determined by the reduction of mitotic index (MI) and induction of chromosome aberration (CA) by the extracts in meristematic cells of <em>Allium</em>. Plant extracts showed a time and dose dependent effect on MI and incidence of CA. Both extracts induced a spectrum of clastogenic as well as non-clastogenic CA. Phytochemical analysis of both extracts revealed the presence of bioactive compounds.</p><p><strong>Conclusion: </strong>Bioactivity of the crude extracts may be ascribed to the active compounds present in the extract as<strong> </strong>indicated by spectroscopic analysis. Further phytochemical investigations may pave a new avenue to cancer drug research. Also, the cytotoxicity of extract apprises the necessity of further studies in the mammalian system.</p><p><strong>Keywords: </strong><em>Orthosiphon thymiflorus, </em>Cytotoxicity, Mitotic Index, <em>Allium cepa</em> Chromosome aberration assay, UV-Vis spectroscopy, FT-IR.</p> ER -