PHYSICOCHEMICAL CHARACTERIZATION OF AN HEMAGGLUTINATING PROTEIN FROM THE FRUIT OF Cola nitida, KOLANUT
AbstractThis study partially purified and characterized an hemagglutinating protein from the fruit of Cola nitida. This protein had heamagglutinating activity towards human erythrocytes (ABO system) and was non-blood group specific. The activity was stable at pH range of 5 â€“ 12, and was inhibited at pHs 3 and 4. The lectin activity was heat stable up to 70 Â°C and was not inhibited by chelating with EDTA.
Slifkin M, Doyle RJ. Lectins and their application to clinical microbiology. Clin Microbiol Rev 1990;3:197-218.
Sharon N. Lectin-carbohydrate complexes of plants and animals: An atomic view. Trends Biochem. Sci 1993;18:221-226
Peumans WJ, Van Damme EJM. Lectins as plant defense proteins. Plant Physiol 1995;109:347-352.
De Wolf FA, Brett GM. Ligand-Binding Proteins: Their Potential or Application in Systems for Controlled Delivery and Uptake of Ligands. Pharmacol Rev 2000;52:207-236.
Hajto T, Hostansaka K, Berki T, Palinkas L, Boldizsar F, Nemeth P. Oncopharmacological perspectives of a plant lectin (Viscum album agglutinin-1): overview of recent results from in vitro experiments and in vivo animal models, and their possible relevance for clinical applications. Evid Based Complement Alternate Medicine (eCAM) 2005;2:59-67.
Delatorre P, Rocha BAM, Souza EM, Oliveira TM, Bezerra GA, Moreno FBMB et al. Structure of a lectin from Canavalia gladiata seeds: New structural insights for old molecules. BMC Struct Biol 2007;7:52.
Chrispeels MJ, Raikhel NV. Lectins, lectins genes, and their role in plant defense. The Plant Cell 1991;3:1-9.
Sharon N, Lis H. Lectins as cell recognition molecules. Science 1989;246:227-234.
Brandley BK, Swiedler SJ, Robbins PW. Carbohydrate ligands of LEC cell adhesion molecules. Cell 1990;63:861-863.
Barral-Netto M, Santos SB, Barral A, Moreira LIM, Santos CF, Moreira RA, et al. Human lymphocytes stimulation by legume lectins from the Dioclea tribe. Immunol Invest 1992;21:297-303.
Konozy EHE, Mulay R, Faca V, Ward RJ, Greene LJ, Roque-Barriera MC, et al. Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin. Biochimie 2002;84:1035-1043.
Konozy EHE, Bernardes ES, Rosa C, Faca V, Greene LJ, Ward RJ. Isolation, purification, and physicochemical characterization of a D-Galactose-binding lectin from seeds of Erythrina speciosa. Arch Biochem Biophys 2003;410:222-229.
Sharon N, Lis H. Lectins (2nd ed.) Kluwer Academic Publishers. London. 2003. 470 pp.
Jayeola CO. Preliminary studies on the use of kolanuts (Cola nitida) for soft drink production. The Journal of Food Technology in Africa 2001;6:25- 26.
Blades M. (2000). Functional foods or neutraceutical. Nutr Food Sci 2001;30:73-75.
Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the Biuret reaction. J Biol Chem 1949; 177: 751-766.
Adeniran OA, Kuku A, Obuotor EM, Agboola FK, Famurewa AJ, Osasan S. Purification, characterization and toxicity of a mannose-binding lectin from the seeds of Treculia africana plant. Toxicol Environ Chem 2009; 91: 1361-1374.
Kuku A, Odekanyin OO, Adeniran K, Adewusi M, Olonade T. Purification of a mannose/glucose-specific lectin with antifungal activity from pepper seeds (Capsicum annuum). Afr J Biochem Res 2009; 3: 272-278.
Adolph L, Lorenz R. A text on enzyme diagnosis in diseases of the heart, liver and pancrease. John Wiley and Sons Inc., New York. 1982. pp. 37-39.
Kaur M, Singh K, Rup PJ, Kamboj SS, Saxena AK, Sharma M, et al. (2006). A tuber lectin from Arisaema jacquemontii blume with anti-insect and anti-proliferative properties. J Biochem Mol Biol 39(4): 432-440.