ANALYTICAL APPLICATIONS OF SAFRANIN O
A simple and sensitive spectrophotometric method has been developed for the estimation of Mycophenolic acid. The method is based on the formation ofÂ Â Ion-Association complex with MYCO formed an ion -association complex with basic dye, SafraninO. The cationic form of the dye SAFO involves in the formation of neutral coloured ion-association complexe with negative charge (acid groups in the drug) which is extractable into chloroform and behaves as a single unit being held together by electrostatic attraction. The absorption maxima were found to be at lMax 520 nm. The method obeys Beer's law within the limits 10-40Âµg/ml and gives reproducible results. Molar absorptivity value is obtained as8.424x104 L mol-1 cm-1 and recovery was found to be 99.17 Â± 0.92 to 99.62 Â± 0.27. Interferences of the other ingredients and excipients were not observed. The proposed method can be used for the determination of MYCO both in pure and pharmaceutical formulations. KeyWords: Mycophenolicacid (MYCO), SafraninO (SAFO), Ion- association Complex
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