IN-VITRO ASSAYS FOR NEUTRALIZATION OF SNAKE VENOM USING HERBAL DRUGS: A REVIEW
Snakebite is a major health hazard that leads to high mortality rate, especially in India. The present review article focuses on the point of view of different inâ€“vitro neutralization assays that serve as an index for assessing the status of therapy. For this purpose bibliographic and scientific literature articles in indexed journal, databases were comprehensively researched, and contemporary articles were studied from different abstracting and indexing systems like PubMed using relevant keywords. It was found that some of the in-vitro assays like Phospholipase A2, Hemorrhagic activity assay, agglutination assay for potency assessment, general proteolytic activity assay, snake venom metalloprotease activity (SVMP), hemolytic activity, neutralization of serum inhibitory activity assay, neutralization of fibrinolytic activity, inhibition of venom cardiotoxic activity assay and brine shrimp lethality test are prevalent in use. Performing these inâ€“vitro tests is essential for the development of therapy against envenomation. Anti snake venom serum (ASVS) for snake bite therapeutics is available but suffers from many drawbacks; herbal plants provide a solid platform for the natural treatment of this serious issue. Herbal medications have excellent potential to treat snake bite. Herbal medicinal plants are an important element of indigenous medical systems globally. Many of the active plant constituents are promising contenders for the development of antivenom drug molecules. So our objective is to find different alternative in-vitro processes for antivenom activity. The rationale behind choosing the in-vitro assays is to enable to generate basic data and understand the probable basic mechanism of snake venom and herbal anti snake venom without sacrificing or using invasive procedures on the experimental animal.
Keywords: Envenomation, Neutralization assays, Inâ€“vitro processes, Hemorrhagic activity, Metalloprotease (SVMP) activity, Brine shrimp lethality test
2. Mallow D, Ludwig D, Nilson G. True vipers: natural history and toxicology of old world vipers. Malabar, Florida: Krieger Publishing Company; 2003. p. 359.
3. SD Aris. Ophidian envenomation strategies and the role of purines. Toxicon 2002;40:335-93.
4. R Murthy Sashidhara, Jagadisha DK, Girisha V, K Kamparaju. Variation in biochemical and pharmacological properties of Indian cobra (Naja naja) venom due to geographical distribution. Mol Cell Biochem 2002;229:93-101.
5. S Schffman, I Thareodor, S I Rapaport. Separation from Russell's viper venom of one fraction reacting with factor X and another reacting with factor V. Biochemistry 1969;8:1397-405.
6. Kini RM, Gowda TV. Inhibition of local effects of Indian Daboia/Vipera russellii venom by the methanolic extracts of grape(Vitis vinifera L.) seeds. Indian J Biochem Biophys 1982;19:342-6.
7. Bishwanath BS, Kini V, Gowda TV. Electrophysiological studies of myotoxin a, isolated from prairie rattlesnake (Crotalus viridis) venom, on murine skeletal muscles. Toxicon 1985;6:929.
8. Jayanthi, TV Gowda. An interdisciplinary journal on the toxins derived from animals, plants and microorganisms. Toxicon 1928;6:65-74.
9. Maung PTM, Gopalkrishnakone R, Tan, Yewn V. A major lethal factor of the venom of burmese russell's viper (Daboia russelli siamensis): Isolation, n-terminal sequencing and biological activities of Daboia toxin. Toxicon 1995;33:73-6.
10. Prasad BN, Kemparaju K, KGS Bhatt, TV Gowda. A platelet aggregation inhibitor phospholipase A2 from russell's viper (vipera russelli) venom: Isolation and characterization. Toxicon 1996;4:1173-85.
11. Chakraborty AK, Datta K, Gomes A, Bhattacharya D. Haemorrhagic protein of Vipera russelli with fibrinolytic and esterolytic activities. Toxicon 2000;38:1475-90.
12. Guiterterrez JM, Leon G, Rojas G, Lomanate B, Rucavado A, Chaves F. Neutralization of local tissue damage induced by Bothrops asper(terciopelo) snake venom. Toxicon 1998; 49:1529-38.
13. Stidworthy J. Snakes of the World. Revised Edition. New York: Grosset and Dunlap Inc; 1974. p. 160.
14. Ditmars RL. Reptiles of the World: The Crocodilians, Lizards, Snakes, Turtles and Tortoises of the Eastern and Western Hemispheres. New York: The McMillan Company; 1937. p. 321.
15. MH Borges, DLF Alves, DS Raslan, D PilÂ´o-Veloso, VM Rodrigues, MI Homsi-Brandeburgo, ME de Limaa. Neutralizing properties of musa paradisiacal L. (Musaceae) juice on phospholipase A2, myotoxic, hemorrhagic and lethal activities of crotalidae venoms. J Economic Perspectives 2000;11;608-17.
16. De Haas GH, Postema NM, Nieuwenhuizen, Van Deenen, L I M. Purification and properties of phospholipase A2 from porcine pancreas. Biochem Biophys Acta 1968;159:103â€“17.
17. Wen-Chan Cxana, Mux-Lnv Leei, Tunc-Bnv Lol, To~Ccn. Phospholipase a2 activity of long-chain cardiotoxins in the venom of the Banded krait(Bungarus fasciatus). Toxicon 1983;21:163-5.
18. PA Shenoy, SS Nipate, JM Sonpetkar, NC Salvi, AB Waghmare, PD Chaudhari. Anti-snake venom activities of ethanolic extract of fruits of Piper longum L. (Piperaceae) against Russell's viper venom: characterization of piper in as active principle. J Ethnopharmacol 2013;147:373â€“82.
19. James E Biardia, Richard G Coss. Rock squirrel (Spermophilus variegatus) blood sera affects proteolytic and hemolytic activities of rattlesnake venoms. Toxicon 2011;57;323â€“31.
20. M I Alam, A Gomes D. Snake venom neutralization by Indian medicinal plants vitexnegundo and emblica officinalis root extracts. J Ethnopharmacol 2003;86;75â€“80.
21. B Adzua, MS Abubakarb, KS Izebec, DD Akumkaa, KS Gamaniela. Effect of Annonasenegalensis root bark extracts on venom in rats. J Ethnopharmacol 2005;96;507â€“13.
22. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen L, Nichols DE, McLaughlin JL. Brine shrimp: a convenient in a general bioassay for active plant constituent. Planta Med 1982;45:31-4.
23. Mackean MM, Ali AM, Lajis NH, Kawazu K, Hassan ZH, Habsah M, et al. Antimicrobial, antioxidant, antitumour-promoting and cytotoxic activities of different plant part of Garcia altroviridis Griffext T. Anderi. J Ethnopharmacol 2000;72:395â€“402.
24. Otero R, Nunez V, Barona J, Fonnegra R, Jimenez SL, Osorio RG, et al. Snakebites and ethnobotany in the northwest region of Colombia. Part III: neutralization of the haemorrhagic effect of Bothropsatroxvenom. J Ethnopharmacol 2000;73:233â€“41.
25. Stocker KF. Composition of snake venoms. In: Medical use of snake venom proteins. CRC Press: Boston; 1990. p. 33.
26. Mahanta M, Mukherjee AK. Neutralization of lethality, myotoxicity and toxic enzymes of Najakaouthiavenom by Mimosa pudicaroot extracts. J Ethnopharmacol 2001;75:55â€“60.
27. Melo PA, Ownby CL. The ability of wedelo lactone, heparin, and para-bromophenacyl bromide to antagonize the myotoxic effects of two crotadine venoms and their PLA2 myotoxins. Toxicon 1999;37:199â€“215.
28. Haslam E. Plants polyphenolsâ€“vegetable tannins revisited. Cambridge University Press: Cambridge; 1989.
29. Adzu B, Abbah J, Vongtau H, Gamaniel K. Studies on the use of Cassia singueanain malaria ethnopharmacy. J Ethnopharmacol 2003b;88:261â€“7.
30. Akunyili DN, Akubue PI. Schumanniofoside, the antisnake venom principle from the stem bark of Schumanninphytonmagnificum Harms. J Ethnopharmacol 1986;18:167â€“72.
31. Okonogi T, Hattori Z, Ogiso A, Mitsui S. Detoxification of persimon tannin of snake venom and bacterial toxins. Toxicon 1979;17:524â€“7.
32. Reyes-Chilpa R, Gomez-Garibay F, Quijano L, Magos-Guerrero G, Rios T. Preliminary results of the protective effect of (âˆ’)-edunol, a pterocarpan from Brongniartia podalyrioides (Leguminosae), against Bothropsatrox venom in mice. J Ethnopharmacol 1994;42:199â€“203.
33. Swinyard EA. Demulcents, emollients, protectives and adsorbents, absorbable hemostatics, astringents, irritants, sclerosing agents, caustics, keratolytic, antiseborrheic, melanizing and demelaninzing agents and certain enzymes. In: Goodman LS, Gilman A. Eds. The Pharmacological Basis of Therapeutics, third ed. Ed. Macmillan, New York, USA; 1965. p. 981â€“2.
34. Haslam E. Natural polyphenols (vegetable tannins) as drugs and medicine: possible modes of action. J Nat Prod 1996;59:205â€“15.
35. Bruno Lomonte, Yamileth Angulo, Mahmood Sasa, JosÃ© MarÃa GutiÃ©rrez. The phospholipase A2 homologues of snake venoms: biological activities and their possible adaptive roles. Protein Pept Lett 2009;16:860-76.