• NEHA GUPTA School of Studies in Biotechnology, Jiwaji University, Gwalior, Madhya Pradesh, India.
  • SAMEER SURESH BHAGYAWANT School of Studies in Biotechnology, Jiwaji University, Gwalior, Madhya Pradesh, India.




Random amplified polymorphic deoxyribonucleic acid-polymerase chain reaction, Kabuli chickpea, Genetic diversity


Objective: Random amplified polymorphic deoxyribonucleic acid-polymerase chain reaction (RAPD-PCR) methodology promoted the development of molecular assay system to detect DNA polymorphisms. Analysis of genetic diversity among exclusive Kabuli accessions collected from IIPR, Kanpur, was demanding.

Methods: The present study apprised application of RAPD in assessing genetic diversity predominantly in Kabuli chickpea seed accessions.

Result: Of the 10 RAPD primers tested, the average number of bands per primer per accession accounted to 9.5. Maximum number of bands scored by the primer OPA-17 was 28 while least number of bands displayed by OPG-04 was 14.

Conclusion: The accession KAK-2 exhibited polymorphic among all and therefore diverse. Thus, it can be utilized as one of the parents in future chickpea breeding program.


Gupta N, Shrivastava N, Bhagyawant SS. Multivariate analysis based on nutritional value, antinutritional profile and antioxidant capacity of forty chickpea genotypes grown in India. J Nutr Food Sci 2017;7:3.

Bhagyawant SS, Gautum AK, Narvekar DT, Gupta N, Bhadkaria A, Srivastava N, et al. Biochemical diversity evaluation in chickpea accessions employing mini-core collection. Physiol Mol Biol Plants 2018;24:1165-83.

Bhagyawant SS, Srivastava N. Assessment of antinutritional factors and protein content in the seeds of chickpea cultivars and their divergence. J Cell Tissue Res 2008;8:1333-8.

Bhagyawant SS, Gupta N, Srivastava N. Biochemical analysis of chickpea accessions vis-a-vis; zinc, iron, total protein, proline and antioxidant activity. Am J Food Sci Technol 2015;3:158-62.

Gautam AK, Shrivastava N, Sharma B, Bhagyawant SS. Current scenario of legume lectins and their practical applications. J Crop Sci Biotechnol 2018;21:217-27.

Peumans WJ, van Damme EJ. Lectins as plant defense proteins. Plant Physiol 1995;109:347-52.

Gautam AK, Gupta N, Narvekar DT, Bhadkaria A, Kumari S, Srivastava N, et al. Chickpea lectin: Purification strategies, characteristics and potential applications. Biochemistry research trends. In: Hemagglutinins: Structures, Functions and Mechanisms. New York: Nova Science Publishers, Inc.; 2019. p. 107-33.

Gautam AK, Srivastava N, Chauhan AK, Bhagyawant SS. Analysis of wild chickpea seed proteins for lectin composition. Int J Curr Res Acad Rev 2017;5:8-14.

Gupta N, Gautam AK, Bhagyawant SS. Biochemical characterization of lectin from wild chickpea (Cicer reticulatum L.) with potential inhibitory action against human cancer cells. J Food Biochem 2018;43:e12712.

Gautam AK, Gupta N, Narvekar DT, Bhadkariya R, Bhagyawant SS. Characterization of chickpea (Cicer arietinum L.) lectin for biological activity. Physiol Mol Biol Plants 2018;24:389-97.

Gautam AK, Srivastava N, Nagar DP, Bhagyawant SS. Biochemical and functional properties of a lectin purified from the seeds of Cicer arietinum L. 3 Biotech 2018;8:272.

Katre UV, Gaikwad SM, Bhagyawant SS, Deshpande UD, Khan MI, Suresh CG. Crystallization and preliminary X‐ray characterization of a lectin from Cicer arietinum (Chickpea). Acta Crystallogr Sect F Struct Biol Cryst Commun 2005;61:141-3.

Bhagyawant SS, Bhadkaria A, Gupta N, Srivastava N. Impact of phytic acid on nutrient bioaccessibility and antioxidant properties of chickpea genotypes. J Food Biochem 2018;42:e12678.

Gupta N, Bisen PS, Bhagyawant SS. Chickpea lectin inhibits human breast cancer cell proliferation and induces apoptosis through cell cycle arrest. Protein Pept Lett 2018;25:492-9.

Gupta N, Bhagyawant SS. Bioactive peptide of Cicer arietinum L. induces apoptosis in human endometrial cancer via DNA fragmentation and cell cycle arrest. 3 Biotech 2021;11:63.

Bhagyawant SS, Narvekar DT, Gupta N, Bhadkaria A, Gautam AK, Srivastava N. Chickpea (Cicer arietinum L.) lectin exhibit inhibition of ACE-I, α-amylase and α-glucosidase activity. Protein Pept Lett 2019;26:494-501.

Bhagyawant SS. Antinutritional factors in pulses and their diversity. Innov J Food Sci 2013;1:27-9.

Bhagyawant SS, Shrivastava N. Recent advances in Plant Molecular Biology. Karnataka: Himalaya Publishing House; 2019. p. 11.

Singh PK, Gautam AK, Akare UR, Bhagyawant SS. Studies on enzymatic changes and their impact on antinutritional factors of pulses during germination. Biosci Biotechnol Res Commun 2014;7:27-31.

Singh PK, Shrivastava N, Sharma B, Bhagyawant SS. Effect of domestic processes on chickpea seeds for antinutritional contents and their divergence. Am J Food Sci Technol 2015;3:111-7.

Bhagyawant SS, Gupta N, Shrivastava N. Effects of gamma irradiation on chickpea seeds vis-a-vis total seed storage proteins, antioxidant activity and protein profiling. Cell Mol Biol 2015;61:79-83.

Singh M, Malhotra N, Singh, K. Broadening the genetic base of cultivated chickpea following introgression of wild Cicer species-progress, constraints and prospects. Genet Resour Crop Evol 2021;127:8.

Welsh J, Mcclelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990;18:7213-8.

Gubae TT, Petros Y. Genetic diversity of chickpea (Cicer arietinum L.) cultivars from Ethiopia by using ISSR markers. J Agric Biotechnol Sustain Dev 2018;10:178-84.

Sadhu S, Jogam P, Thampu RK, Abbagani S, Penna S, Peddaboina V. High efficiency plant regeneration and genetic fidelity of regenerants by SCoT and ISSR markers in chickpea (Cicer arietinum L.). Plant Cell Tiss Organ Cult 2020;141:465-77.

Yadav P, Koul KK, Shrivastava N, Mendaki MJ, Bhagyawant SS. DNA polymorphisms in chickpea accessions as revealed by PCR-based markers. Cell Mol Biol 2015;61:84-90.

Bhagyawant SS, Gupta N, Gautam A, Chaturvedi SK, Shrivastava N. Molecular diversity assessment in chickpea through RAPD and ISSR markers. World J Agric Res 2015;3:192-7.

Yadav P, Koul KK, Shrivastava N, Mendaki MJ, Bhagyawant SS. ITS-PCR deciphers molecular phylogeny in chickpea. Plant Biosyst 2016;151:429-35.

Valadez-Moctezuma E, Cabrera-Hidalgo AJ. Easy strategy used to detect the genetic variability in chickpea (Cicer arietinum L.). Physiol Mol Biol Plants 2018;24:921-8.

Talebi R, Fayaz F, Mardi M, Pirsyedi SM, Naji AM. Genetic relationships among chickpea (Cicer arietinum) elite lines based on RAPD and agronomic markers. Int J Agri Biol 2008;10:301-5.

Jaccard P. New research on flower distribution. Bull Soc Vaudoise Sci Nat 1908;44:223-70.

Pavlicek A, Hrda S, Flegr J. Free tree: Freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol (Praha) 1999;45:97-9.

Bhagyawant SS, Srivastava N. Genetic fingerprinting of chickpea (Cicer arietinum L.) germplasm using ISSR markers and their relationships. Afr J Biotechnol 2008;7:4428-31.

Bhagyawant SS, Srivastava N, Koul KK. Diversity in chickpea and its wild relatives based on seed protein profiles. Crop Res 2008;37:168-73.

Singh PK, Kumar A, Srivastava N, Agarwal RM, Bhagyawant SS. Association of protein profiling and agronomic traits in chickpea as revealed using SDS-PAGE. J Cell Tissue Res 2012;12:3279-84.

Singh PK, Shrivastava N, Chaturvedi K, Bhagyawant SS. Characterization of seed storage proteins from chickpea using 2D electrophoresis coupled with mass spectrometry. Biochem Res Int 2016;2016:1049462.

Gupta N, Srivastava N, Singh PK, Bhagyawant SS. Phytochemical evaluation of moth bean (Vigna aconitifolia L.) seeds and their divergence. Biochem Res Int 2016;2016:3136043.

Gupta, N, Srivastava N, Bhagyawant SS. Vicilin-a major storage protein of mungbean exhibits antioxidative potential, antiproliferative effects and ACE inhibitory activity. PLoS One 2018;13:e0191265.

Bhadkaria A, Srivastava N, Bhagyawant SS. A prospective of underutilized legume moth bean (Vigna aconitifolia (Jacq.) Marechàl): Phytochemical profiling, bioactive compounds and in vitro pharmacological studies. Food Biosci 2021;2021:101088.

Gupta N, Bhagyawant SS. Impact of hydrolysis on functional properties, antioxidant, ACE-I inhibitory and anti-proliferative activity of Cicer arietinum and Cicer reticulatum hydrolysates. Nutrire 2019;44:5.

Gupta N, Bhagyawant SS. Enzymatic treatment improves ACE-I inhibiton and antiproliferative potential of chickpea. Vegetos 2019;32:363-9.

Gupta N, Bhagyawant SS. Angiotensin-I converting enzyme (ACE-I) inhibitory and antiproliferative potential of chickpea seed protein hydrolysate. Ann Plant Sci 2018;7:2149-53.

Gautam AK, Gupta N, Srivastava N, Bhagyawant SS. Proteomic analysis of chickpea roots reveal differential expression of abscisic acid responsive proteins. J Food Biochem 2019;43:e12838.

Bhagyawant SS, Narvekar DT, Gupta N, Bhadkaria A, Koul KK, Srivastava N. Variations in the antioxidant and free radical scavenging under induced heavy metal stress expressed as proline content in chickpea. Physiol Mol Biol Plants 2019;25:683-96.

Bhagyawant SS. RAPD-SCAR markers: An interface tool for authentication of traits. J Biosci Med 2015;4:1-9.

Singh PK, Sharma H, Srivastava N, Bhagyawant SS. Analysis of genetic diversity among wild and cultivated chickpea genotypes employing ISSR and RAPD markers. Am J Plant Sci 2014;5:676-82.

Gautam AK, Gupta N, Bhadkariya R, Srivastava N, Bhagyawant SS. Genetic diversity analysis in chickpea employing ISSR markers. Agrotechnology 2016;5:1000152.

Bhadkaria A, Gupta N, Narvekar DT, Bhadkariya R, Saral A, Srivastava N, et al. ISSR-PCR approach as a means of studying genetic variation in moth bean (Vigna aconitifolia (Jacq.) Maréchal). Biocatal Agric Biotechnol 2020;30:101827.



How to Cite

GUPTA, N., & BHAGYAWANT, S. S. (2021). RAPD ANALYSIS IN KABULI CHICKPEA SEED ACCESSIONS. Innovare Journal of Agricultural Sciences, 9(4), 6–9. https://doi.org/10.22159/ijags.2021.v9i4.42183



Original Article(s)