GENETIC RELATEDNESS AMONG MAIZE INBRED LINES BASED ON ISSR MARKERS AND ITS ASSOCIATION WITH HETEROSIS AND HYBRID PERFORMANCE

Authors

  • AHMED MEDHAT MOHAMED AL-NAGGAR Department of Agronomy, Faculty of Agriculture, Cairo University, Giza, Egypt.
  • AHMED MONGED SOLIMAN Department of Agronomy, Faculty of Agriculture, Cairo University, Giza, Egypt.
  • MONA HASHEM HUSSIEN Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt.
  • SHAFIK DARWISH IBRAHIM Department of Genome Mapping, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt.
  • ELHAM MOHAMED HASSAN MOHAMED Department of Agronomy, Faculty of Agriculture, Omar Elmokhtar University, Al Bayda’, Libya.

DOI:

https://doi.org/10.22159/ijags.2022.v10i6.46548

Keywords:

Zea mays, ISSR, genetic diversity, heterosis

Abstract

The objectives of the present study were to measure the genetic diversity among eight inbred lines of maize using ISSR markers and the correlation coefficients between genetic diversity and each of heterosis and mean performance of hybrids for grain yield. Ten ISSR primers were used in the detection of polymorphism of the eight inbred lines in a laboratorial experiment. Heterosis and mean performance of grain yield/ha in their F1 diallel crosses were measured in a 2-year field experiment using a randomized complete blocks design with three replications. Based on ISSR markers, the genetic similarity coefficients among the eight maize inbred lines ranged from 0.798 (between L17 and IL53) to 0.943 (between IL80 and IL84) with an average of 0.869. Unique bands associated with maize inbred lines were identified. The results revealed that the genetic diversity among the inbred lines based on ISSR markers showed a significant, and negative relationship with mid-parent heterosis and mean performance of grain yield/ha. Further, intensive investigation of a large set of maize inbred lines from diverse populations using a large number of ISSR primers is required for proper understanding of genetic diversity of maize crop. Findings will be valuable for maize breeder, to practice effective selection of parental inbred lines for obtaining maximum heterosis and high mean grain yield/ha in their hybrids.

Author Biographies

AHMED MONGED SOLIMAN, Department of Agronomy, Faculty of Agriculture, Cairo University, Giza, Egypt.

Professor of plant breeding, Agronomy department, Faculty of Agriculture, Cairo  university

MONA HASHEM HUSSIEN, Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt.

Professor, Genetics Department, Faculty of Agric., Cairo University, Egypt

SHAFIK DARWISH IBRAHIM, Department of Genome Mapping, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt.

Department of Genome Mapping, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt.

ELHAM MOHAMED HASSAN MOHAMED, Department of Agronomy, Faculty of Agriculture, Omar Elmokhtar University, Al Bayda’, Libya.

Department of Agronomy, Faculty of Agriculture, Omar Elmokhtar University, Libya

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Published

17-10-2022

How to Cite

AL-NAGGAR, A. M. M., SOLIMAN, A. M., HUSSIEN, M. H., IBRAHIM, S. D., & HASSAN MOHAMED, E. M. (2022). GENETIC RELATEDNESS AMONG MAIZE INBRED LINES BASED ON ISSR MARKERS AND ITS ASSOCIATION WITH HETEROSIS AND HYBRID PERFORMANCE. Innovare Journal of Agricultural Sciences, 10(6), 5–9. https://doi.org/10.22159/ijags.2022.v10i6.46548

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Original Article(s)