ANALYSIS OF CHLOROPHYLL AND Β-CAROTENE CONTENT OF WINTER VEGETABLES
DOI:
https://doi.org/10.22159/ijags.2025v13i1.48985Keywords:
Chlorophyll, β-carotene, Vegetables, Cluster analysis, Distance matrix, Correlation coefficientAbstract
As a primary pigment of leafy green vegetables, chlorophyll plays a major role in indicating vegetable growth status. Carotenoids are natural fat-soluble pigments that are common in colorful plants. They act as provitamin A and are beneficial for human health, especially for vision. An experiment was conducted to determine the chlorophyll and β-carotene content of winter vegetables. The chlorophyll and β-carotene content of fresh vegetables was determined using the spectrophotometer method. Chlorophyll b content in winter vegetables ranged from 0.07 mg/100 g in beetroot to 29.76 in roselle fruit. It was found that radish leaf (54.91±0.795 mg/100 g) is very rich in total chlorophyll followed by spinach (54.28 mg/100 g), goosefoot (49.97 mg/100 g), roselle leaf (40.38 mg/100 g), and roselle fruit (34.04 mg/100 g) among the studied winter vegetables. The β-carotene content in winter vegetables ranged from 3.07 μg/100 g in beetroot to 1060.13 μg/100 g in radish leaf. This study has shown that radish leaf (1060.13 μg/100 g) is very rich in β-carotene followed by goat weed (964.84 μg/100 g), spinach (887.44 μg/100 g), carrot (630.98 μg/100 g), and goosefoot (563.21 μg/100 g). Cluster II included spinach, radish leaf, and goat weed with high chlorophyll and β-carotene content of winter vegetables. Euclidean distance between cabbage and sorrel was the lowest (16.09) while the largest distance was observed between beetroot and radish leaf (1059.18). A highly significant positive correlation was observed between β-carotene and chlorophylls. Radish leaf, spinach, goosefoot, roselle leaf, and roselle fruit were observed to have a high total chlorophyll content as well as radish leaf, goat weed, spinach, carrot, and goosefoot were observed to have high β-carotene content among the studied winter vegetables. They should be regularly included in the diet for the adequate supply of chlorophyll and β-carotene in the winter season for the protection of our health from diseases.
References
Andrejiová, A., & Mendelová, A. (2012). Effect of Variety on the Chlorophyll Conten in Fresh Leaves of Spinach (Spinacia oleracea L.) and Spinach Purée. In Horticulture nitra 2012. International reviewed proceedings of scientific papers (pp. 23-27). Nitra: SUA.
Antonio, F., Luca, I., Rita, M., Giovanni, S., & Franco, T. (2004). Colour changes of fresh-cut leafy vegetables during storage. Journal of Food Agriculture and Environment, 2(3-4), 40-44.
Bhaskarachary, K., Ananthan, R., & Longyah, T. (2008). Carotene content of some common (cereals, pulses, vegetables, spices and condiments) and unconventional sources of plant origin. Food Chemistry, 106, 85-89.
Boekel, M. A. J. S. (1999). Testing of kinetic models: Usefulness of the multiresponse approach as applied to chlorophyll degradation in foods. Food Research International, 32(4), 261-269.
Caldwell, C. R., & Britz, S. J. (2006). Effect of supplemental ultraviolet radiation on the carotenoid and chlorophyll composition of green house-grown leaf lettuce (Lactuca sativa L.) cultivars. Journal of Food Composition and Analysis, 19, 637-644.
Faller, A. L. K., & Fialho, E. (2009). The antioxidant capacity and polyphenol content of organic and conventional retail vegetables after domestic cooking. Food Research International, 42(1), 210-215.
Grunwald, C., Sims, J. L., & Sheen, S. J. (1977). Effects of nitrogen fertilization and stalk position on chlorophyll, carotenoids, and certain lipids of three tobacco genotypes. Canadian Journal of Plant Science, 57, 525-535.
Ihl, M., Shene, C., Scheuermann, E., & Bifani, V. (1994). Correlation for pigment content through colour determination using tristimulus values in a green leafy vegetable, Swiss chard. Journal of the Science of Food and Agriculture, 66, 527-531.
Ihl, M., Shene, C., Scheuermann, E., & Bifani, V. (2006). Correlation for pigment content through colour determination using tristimulus values in a green leafy vegetable, Swiss chard. Journal of the Science of Food and Agriculture, 66, 527-531.
Johnson, E. J., Hammond, B. R., Yeum, K. J., Qin, J., Wang, X. D., Castaneda, C., Snodderly, D. M., & Russell, R. M. (2000). Relation among serum and tissue concentrations of lutein and zeaxanthin and macular pigment density. The American Journal of Clinical Nutrition, 71, 1555-1562.
Kimura, M., & Rodriguez-Amaya, D. B. (2002). A scheme for obtaining standards and HPLC quantification of leafy vegetable carotenoid. Food Chemistry, 78(3), 389-398.
Kopsell, D. A., Kopsell, D. E., & Lefsrud, M. G. (2004). Variation in lutein, b-carotene, and chlorophyll concentrations among Brassica oleraceae cultings and seasons. HortScience, 39, 361-364.
Larsen, E., & Christensen, L. P. (2005). Simple saponification method for the quantitative determination of carotenoids in green vegetables. Journal of Agricultural and Food Chemistry, 53(17), 6598-6602.
Le Marchand, L., Hankin, J. H., Kolonel, L. N., Beecher, G. R., Wilkens, L. R., & Zhao, L. P. (1993). Intake of specific carotenoids and lung cancer risk. Cancer Epidemiology, Biomarkers and Prevention, 2, 183-187.
Lopez-Ayerra, B., Murcia, M. A., & Garcia-Carmona, F. (1998). Lipid peroxidation and chlorophyll levels in spinach during refrigerated storage and after industrial processing. Food Chemistry, 61(1-2), 113-118.
Marković M., Pavlović D., Tošić S., Stankov Jovanović V., Krstić N., Stamenković S., Mitrović T., & Marković V. (2012). Chloroplast pigments in post-fire-grown Cryptophytes on Vidlič mountain (Southeastern Serbia). Archives of Biological Sciences, 64(2), 531-538.
Mortensen, A., Skibsted, L. H., & Truscott, T. G. (2001). The interaction of dietary carotenoids with radical species. Archives of Biochemistry and Biophysics, 385(1), 13-19.
Nagata, M., Dan, K., & Yamashita, I. (1992). Simple method of simultaneous determination of chlorophyll and carotenoides in tomato. Journal of the Japanese Society for Horticultural Science, 61(2), 686-687.
Olson, J. A. (1994). Carotenoids: Absorption, transport, and metabolism of carotenoids in humans. Pure and Applied Chemistry, 66, 1011-1016.
Podsedek, A. (2007). Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Food Science and Technology, 40, 1-11. Pritwani, R., & Mathur, P. (2017). β-carotene content of some commonly consumed vegetables and fruits available in Delhi, India. Journal of Nutrition and Food Sciences, 7, 625.
Richard, A. J., & Dean, W. W. (2002). Applied multivariate statistical analysis. London: Prentice-Hall.
Sangeetha, R. K., & Baskaran, V. (2010). Carotenoid composition and retinol equivalent in plants of nutritional and medicinal importance: Efficacy of b-carotene from Chenopodium album in retinol-deficient rats. Food Chemistry, 119, 1584-1590.
Sheoran, O. P., Tonk, D. S., Kaushik, L. S., Hasija, R. C., & Pannu, R. S. (1998). Statistical software package for agricultural research workers. In D. S. Hooda & R. C. Hasija (Eds.), Recent advances in information theory, statistics and computer applications (pp. 139-143). Hisar: CCS HAU.
Terry, N., & Abadía, J. (1986). Function of iron in chloroplasts. Journal of Plant Nutrition, 9(3-7), 609-646.
Tonucci, L. H., & Von Elbe J. H. (1992). Kinetics of the formation of zinc complexes of chlorophyll derivatives. Journal of Agricultural and Food Chemistry, 40(12), 2341-2344.
Turkmen, N., Poyrazoglu, E. S., Sari, F., & Sedat Velioglu, Y. (2006). Effects of cooking methods on chlorophylls, pheophytins and colour of selected green vegetables. International Journal of Food Science and Technology, 41(3), 281-288.
Wold, S. (1995). Chemometrics; What do we mean with it, and what do we want from it? Chemometrics and Intelligent Laboratory Systems, 30(1), 109-115.
Woodcock, T., Downey, G., Kelly, J. D., & O’Donnell, C. (2007). Geographical classification of honey samples by near-infrared spectroscopy: A feasibility study. Journal of Agricultural and Food Chemistry, 55(22), 9128-9134.
Xue, L., & Yang, L. (2009). Deriving leaf chlorophyll content of green-leafy vegetables from hyperspectral reflectance. ISPRS Journal of Photogrammetry and Remote Sensing, 64(1), 97-106.
Xuelian, Z., Zhaoqi, Z., Jin, L., Lajie, W., Jiongye, G., Lvqing, O., Yinyin, X., Xuemei, H., & Xuequn, P. (2011). Correlation of leaf senescence and gene expression/activities of chlorophyll degradation enzymes in harvested Chinese flowering cabbage (Brassica rapa var. parachinensis). Journal of Plant Physiology, 168(11), 2081-2087.
Yoshida, S., Forno, D. A., Cock, J. H., & Gomez, K. (1996). Determination of chlorophyll in plant tissue. In S. Yoshida, D. A. Forno, J. H. Cock & K. A. Gomez (Eds.), Laboratory manual for physiological studies of rice, (3rd ed.) (Ch. 10) (pp. 43-45). Los Banos, Philippines: The International Rice Research Institute.
Zhang, D., & Hamauzu, Y. (2004). Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking. Food Chemistry, 88(4), 503-509.
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