PREPARATION AND CHARACTERIZATION OF CEFTRIAXONE SODIUM ENCAPSULATED CHITOSAN NANOPARTICLES


P. Manimekalai, R. Dhanalakshmi, R. Manavalan

Abstract


Objective: The objective of this study was to prepare ceftriaxone sodium chitosan nanoparticles (CS-NP) from different drug and polymer ratios and analyze their physicochemical characteristics.

Methods: Ceftriaxone sodium loaded chitosan nanoparticles were prepared using chitosan as a polymer and tri sodium polyphosphate (TPP) as cross linking agent by ionic cross linking and coacervation with the aid of sonication. Various trials have been carried out for the confirmation of nanoformulation. Parameters such as the zeta potential, polydispersity, particle size, entrapment efficiency, in vitro drug release Thermo gravimetric analysis and scanning electron microscope of the nanoparticles were assessed for confirmation of nanoformulation.

Results: The formulated nanoparticles showed mean particle size, polydispersity index and zeta potential to be 183.1±8.42 nm, 0.212±0.05, +38.5±1.6 mV respectively and the drug loading was found to be 46.42±10 %. In vitro drug release was showed a biphasic release pattern with initial burst release followed by sustained release of formulated nanoparticles. The cumulative percentage of drug release was about 83.08 %.

Conclusion: Formulation F2 was found to be the best formulation with a higher cumulative percentage of drug release. Modified ionic gelation method can be utilized for the development of chitosan nanoparticles of ceftriaxone sodium. Polymer and crosslinking agent concentrations and sonication time are rate-limiting factors for the development of the optimized formulation. The chitosan nanoparticles developed would be capable of sustained delivery of ceftriaxone sodium.


Keywords


Ceftriaxone sodium, Chitosan nanoparticles, Zeta potential analysis, Scanning Electron Microscope Analysis, Particle size

| PDF | HTML |

References


Lipinski CA, Lombardo F, Dominy BW, Feeney PJ. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Adv Drug Delivery Rev 2001;46:3–26.

Lee S, Kim SK, Lee DY, Park K, Kumar TS, Chae SY, et al. Cationic analog of deoxycholate as an oral delivery carrier for ceftriaxone. J Pharm Sci 2005;94:8-18.

Cho SW, Lee JS, Choi SH. Enhanced oral bioavailability of poorly absorbed drugs. I. Screening of absorption carrier for the ceftriaxone complex. J Pharm Sci 2004;93:612–20.

Shanmugarathinam A. Puratchikody, formulation and characterisation of ritonavir loaded ethylcellulose buoyant microspheres. J Pharm Sci Res 2014;8:274-7.

Gan Q, Wang T, Cochrane C, McCarron P. Modulation of surface charge, particle size and morphological properties of chitosan TPP nanoparticles intended for gene delivery. Colloids Surf B 2005;44:65–73.

Ishak RAH, Awad GAS, Mortada ND, Nour SA. Preparation, in vitro and in vivo evaluation of stomach specific metronidazole loaded alginate beads as local anti helicobacter pylori therapy. J Controlled Release 2007;119:207-14.

Rao JP, Kurt E, Meckler KE. Polymer nanoparticles: preparation techniques and size-control parameters. Prog Polym Sci 2011;36:887-913.

S Sakhare, AV Yadav, PD Jadhav. Design, development and characterization of mucoadhesive gastro spheres of carvedilol. Int J Appl Pharm 2016;3:37-42.

Hu FQ, Meng P, Dai YQ, Du YZ, You J, Wei XH, et al. PEGylated chitosan-based polymer micelle as an intracellular delivery carrier for anti-tumor targeting therapy. Eur J Pharm Biopharm 2008;70:749–57.

Chen X, WJ Li, TY Yu. Conformation transition of silk fibroin induced by blending chitosan. J Polymer Sci B 1997;35:2293-6.

Chiou WL, Riegelman S. Pharmaceutical applications of solid dispersion systems. J Pharm Sci 1971;60:1281-302.

El-Shabouri MH. Positively charged nanoparticles for improving the oral bioavailability of cyclosporine. Int J Pharm 2002;249:101.

Venkateswarlu V, Manjunath K. Preparation, characterization and in vitro release kinetics of clozapine solid lipid nanoparticles. J Controlled Release 2004;95:627–38.

Moghimi SM, Hunter AC, Murray JC. Nanomedicine: current status and future prospects. FASEB J 2005;19:300-11.

Bretschneider B, Brandsch M, Neubert R. Intestinal transport of beta-lactam antibiotics: analysis of the affinity at the H+/peptide symporter (PEPT1), the uptake into Caco-2 cell monolayers andtransepithelial flux. Pharm Res 1999;16:55–61.

Nasti A, Zaki NM, de Leonardis P, Ungphaiboon S, Sansongsak P, Rimoli MG, et al. Chitosan/TPP and chitosan/TPP-hyaluronic acid nanoparticles: systematic optimisation of the preparative process and preliminary biological evaluation. Pharm Res 2009;26:1918–30.

J Joysa Ruby, VP Pandey. Chitosan nanoparticles as a nasal drug delivery for memantine hydrochloride. Int J Pharm Pharm Sci 2015;1:34-7.

Giron D. Application of thermal analysis in the pharmaceutical industry. J Pharm Biomed Anal 1986;4:755-70.

Y Kawashima, T Handa, A Kasai, H Takenaka, SY Lin. Novel method for the preparation of controlled-release theophylline granules coated with a polyelectrolyte complex of sodium polyphosphate-chitosan. Chem Pharm Bull 1985;33:2469–74.

P Manimekalai, R Manavalan. Selection of excipients for the formulation of ceftriaxone sodium loaded chitosan nanoparticle through drug-excipient compatibility testing. Int J PharmTech Res 2015;8:5-10.

P Manimekalai, R Manavalan. Moleculardocking studies of ceftriaxone sodium with apoptosis protein in colorectal cancer. Int J Res Pharm Sci 2014;5:250-5.

Elizabath Antony, Mythili Sathiavelu, Sathiavelu Arunachalam. Synthesis of silver nanoparticles from the medicinal plant bauhinia acuminata and biophytum sensitivum–a comparative study of its biological activities with plant extract. Int J Appl Pharm 2017;9:22-9.

Pannerselvam, Balashanmugam, Pudupalayam T, Kalaichelvan. Biosynthesis characterization of silver nanoparticles using cassia roxburghii DC aqueous extract and coated on cotton cloth for effective antibacterial activity. Int J Nanomed 2015;10:87-97.

Arulmozhi K, Pandian S, Mirunalinia ellagic acid encapsulated chitosan nanoparticles for drug delivery system in human oral cancer cell line (KB) 3. Colloids Surf B 2013;110:313–20.

Ohya Y, Shiratani M, Kobayashi H, Ouchi T. Release behaviour of 5-fluorouracil from chitosan-gel nanospheres immobilizing 5-fluorouracil coated with polysaccharides and their cell specific cytotoxicity. Pure Appl Chem 1994;31:629-42.

Lifeng Qi, Zirong Xu, Xia Jiang, Caihong Hu, Xiangfei Zou. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydrate Res 2004;339:2693–700.

Simar Preet Kaur, Rekha Rao, Afzal Hussain, Sarita Khatkar. Preparation and characterization of rivastigmine loaded chitosan nanoparticles. J Pharm Sci Res 2011;5:1227-32.

Elkholi IE, Hazem NM, ElKashef WF, Sobh MA, Shaalan D. Evaluation of anti-cancer potential of capsaicin-loaded trimethyl chitosan-based nanoparticles in HepG2 hepatocarcinoma cells. J Nanomed Nanotechnol 2014;5:240-5.

Anbarasan B, Vennya V Menon, Niranjana VA, Ramprabhu. Optimization of the formulation and in-vitro evaluation of chloroquine loaded chitosan nanoparticles using ionic gelation method. J Chem Pharm Sci 2013;6:407-12.




About this article

Title

PREPARATION AND CHARACTERIZATION OF CEFTRIAXONE SODIUM ENCAPSULATED CHITOSAN NANOPARTICLES

Keywords

Ceftriaxone sodium, Chitosan nanoparticles, Zeta potential analysis, Scanning Electron Microscope Analysis, Particle size

DOI

10.22159/ijap.2017v9i6.16317

Date

08-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 9, Issue 6 (Oct-Nov), 2017 Page: 10-15

Online ISSN

0975-7058

Statistics

47 Views | 16 Downloads

Authors & Affiliations

P. Manimekalai
School of Pharmaceutical Sciences, VELS University, Pallavaram,Chennai-117
India

R. Dhanalakshmi
E.G.S Pillai College of Pharmacy, Nagapattinam 611002

R. Manavalan
RVS College of Pharmaceutical Sciences, Sulur, Coimbatore 641402


Refbacks

  • There are currently no refbacks.