• Kurniawansyah I. S. Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy Padjadjaran University, Indonesia
  • Sulistyaningsih .
  • Ramadhani N. Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia


Objective: The aim of the present study was to know the activity of injection preparation of gentamicin sulfate in varied intravenous fluids (NaCl and Dextrose-NaCl) as well as variation of time of use against bacteria Bacillus subtilis and Klebsiella pneumoniae.

Methods: The research was a laboratory experiment with the following stages: 1.) mixing the sample performed in LAF, 2.) antibacterial activity test with time variation against Bacillus subtilis and Klebsiella pneumoniae bacteria, and 3.) analysis with ANOVA was needed to see if the infusion variation and time variation had a significant effect on gentamicin sulfate activity in killing Bacillus subtilis and Klebsiella pneumoniae bacteria.

Results: The mixing of gentamicin sulphate in both intravenous fluids for 2 h has a diameter of more than 20 mm inhibition. Gentamicin sulfate has decreased activity when mixed with intravenous fluids, both NaCl 0.9% and Dextrose 5%-NaCl 0.225%. In both samples, there was a decrease in the inhibitory zone diameter of each mixing time increase. The first minute of mixing time gave the best effect, and the infusion variation had a significant effect only on gentamicin sulphate activity against Bacillus subtilis bacteria. Intravenous fluids Dextrose-NaCl gave a better effect than infusion of NaCl.

Conclusion: NaCl Infusion and Dextrose-NaCl had no effect on the activity of injection preparation of gentamicin sulfate against Klebsiella pneumoniae bacteria, but had an effect on Bacillus subtilis. The time of mixed use of gentamicin sulfate injection with NaCl and Dextrose-NaCl infusion fluid gave effect to its activity on Bacillus subtilis and Klebsiella pneumoniae bacterias, where the longer mixing time gentamicin sulphate activity decreased.

Keywords: Antibacterial, Gentamicin sulphate, Dextrose-NaCl, Bacillus subtilis, Klebsiella pneumonia


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How to Cite
S., K. I., ., S., & N., R. (2018). THE ACTIVITY OF DOSAGE INJECTION IN GENTAMICIN SULPHATE IN LIQUID INFUSION NaCl AND DEKSTROSA-NaCl ON BACTERIA Bacillus subtilis ATCC 6633 AND Klebsiella pneumoniae ATCC 2357. International Journal of Applied Pharmaceutics, 10(1), 53-58.
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