DESIGN, FORMULATION AND EVALUATION OF LIPOSOME CONTAINING ISONIAZID


Akshay Singha Roy, Sudipta Das, Arnab Samanta

Abstract


Objective: The objective of the present study was to formulate and evaluate liposomes loaded with isoniazid.

Methods: Liposome of isoniazid was made by thin layer film hydration method. L-α-phosphatidylcholine and cholesterol were used to make multiamellar vesicles. Six batches of liposomes were prepared based on the different weight ratio of L-α-phosphatidylcholine and cholesterol. Differential scanning calorimetry (DSC) study conducted to study in any incompatibility.

Results: The prepared liposomes were evaluated by particle size analysis, entrapment efficiency, release study and stability study. Particle sizes were determined from the scanning electron microscopy (SEM) photographs. When particle frequencies were plotted against particle diameter in the histogram, it showed that F1 batch had a skewed distribution towards smaller liposomes while F6 shows a proper bell-shaped curve with a mean at 225 mm. The percentage entrapment efficiency was found to be 8.99 ± 0.15 to 4.19 ± 0.12 % respectively. From the release profile, it was seen that F1 batch was fastest and F6 was slowest to release the drug. The satisfactory batch F1 was packed in Eppendorf tube and stored at 4 °C temperature for one month. At the end of one month, the samples were analyzed for their physical properties, drug entrapment and in vitro release profile. The percentage release was found to be 96.5 ± 3.2 after 4 h.

Conclusion: The F1 batch showed most promising results compared to other. No significant change was found during one month’s stability study of final batch (F1).


Keywords


Liposome, Isoniazid, L-α-phosphatidylcholine, Cholesterol, Stability

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About this article

Title

DESIGN, FORMULATION AND EVALUATION OF LIPOSOME CONTAINING ISONIAZID

Keywords

Liposome, Isoniazid, L-α-phosphatidylcholine, Cholesterol, Stability

DOI

10.22159/ijap.2018v10i2.24174

Date

07-03-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 2 (Mar-Apr), 2018 Page: 52-56

Online ISSN

0975-7058

Authors & Affiliations

Akshay Singha Roy
PG Research Laboratories, Department of Pharmaceutics, Netaji Subhas Chandra Bose Institute of Pharmacy, Chakdaha 741222, Nadia, West Bengal, India
India

Sudipta Das
PG Research Laboratories, Department of Pharmaceutics, Netaji Subhas Chandra Bose Institute of Pharmacy, Chakdaha 741222, Nadia, West Bengal, India
India

Arnab Samanta
PG Research Laboratories, Department of Pharmaceutics, Netaji Subhas Chandra Bose Institute of Pharmacy, Chakdaha 741222, Nadia, West Bengal, India
India


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