NEUROTOXIC EFFECT OF TITANIUM DIOXIDE NANOPARTICLES: BIOCHEMICAL AND PATHOLOGICAL APPROACH IN MALE WISTAR RATS


D. Vasantharaja, V. Ramalingam

Abstract


Objective: Titanium dioxide nanoparticles (TiO2 NPs) are widely used in pharmaceutical, cosmeceutical, biomedical and industrial applications. The adverse effects of TiO2 NPs are also increasing alarmingly. The purpose of this study is to investigate the toxicity of TiO2 NPs on biochemical and histological changes in different regions of brain in adult male Wistar rats.

Methods: Two different doses of TiO2 NPs (50 mg/kg b. w and 100 mg/kg b. w) administered orally for 14 d along with one control group, each group consisting of six animals. Standard biochemical methods were adopted for the estimation of enzymes alkaline phosphatase, 5’ nucleotidase, ATPases and gamma-glutamyl transpeptidase. Trace elements calcium, sodium, potassium and magnesium as well as metals like iron, zinc and copper were also estimated.

Results: When compared with the control group, the enzymes ATPases, ALP, 5’-NT and GGT activities were significantly decreased in both the TiO2 NPs treated groups. Ca, Na, Fe, Cu and TiO2 contents were significantly increased in both the experimental groups, while the K, Mg and Zn contents decreased. However, the changes in the parameters studied were more in 100 mg treated group (p<0.001) when compared to the 50 mg treated group (p<0.05and p<0.01). Moreover, it is also evident that different regions responded differently due to TiO2 NPs exposure. The changes were maximum in the cerebral hemisphere (p<0.001) followed by medulla oblongata (p<0.001) and cerebrum (p<0.05).

Conclusion: The results clearly imply that TiO2 NPs could impair the electrochemical gradient, ionic homeostasis and membrane stability in different regions of the rat brain.


Keywords


Nanoparticles, Titanium, Brain, Membrane enzymes, Trace metals

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About this article

Title

NEUROTOXIC EFFECT OF TITANIUM DIOXIDE NANOPARTICLES: BIOCHEMICAL AND PATHOLOGICAL APPROACH IN MALE WISTAR RATS

Keywords

Nanoparticles, Titanium, Brain, Membrane enzymes, Trace metals

DOI

10.22159/ijap.2018v10i4.25622

Date

07-07-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 4 (July-Aug), 2018 Page: 74-81

Online ISSN

0975-7058

Authors & Affiliations

D. Vasantharaja
Department of Zoology, Kanchi Mamunivar Centre for Post Graduate Studies (Pondicherry University), Lawspet, Puducherry-605008. India
India

V. Ramalingam
Department of Zoology, Kanchi Mamunivar Centre for Post Graduate Studies (Pondicherry University), Lawspet, Puducherry-605008. India
India


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