DEVELOPMENT AND VALIDATION OF UV-VIS SPECTROSCOPIC METHOD OF ASSAY OF CARBAMAZEPINE IN MICROPARTICLES

  • Saeid Mezail Mawazi Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan campus, Malaysia School of Pharmacy, PICOMS International University College, Batu Muda, Batu Caves, Kuala Lumpur, Malaysia
  • Hazrina A. B. Hadi Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan campus, Malaysia
  • Sinan Mohammed Abdullah Al-mahmood Department of Basic Medical Science, Kulliyyah of Nursing, International Islamic University Malaysia, Kuantan campus, Malaysia
  • Abd Almonem Doolaanea IKOP Sdn Bhd, Kulliyyah of Pharmacy, International Islamic University Malaysia

Abstract

Objective: This study aimed to develop a new, rapid, robust, effective, inexpensive, and accurate UV-Vis method for the quantification analysis of carbamazepine (CBZ) in the carbamazepine-loaded microparticles.

Methods: CBZ was encapsulated in ethyl cellulose microparticles by a solvent evaporation method using polyvinyl alcohol (PVA) as a stabilizer. Methanol was used to dissolve CBZ followed by dilution with distilled water as diluent. CBZ drug, excipients, and microparticles were subjected to specificity, solution stability, linearity, precision and accuracy to confirm and ensure the validity of this method.

Results: The results showed no interference from the excipients in the selected wavelength 286 nm. It was exhibited linearity in the range 2-12 μg/ml with R2 = 0.9992. CBZ solution was stable during 24 h. Accuracy and precision were within the accepted limits (100±2%). All results were in accordance to the ICH-Q2 guideline.

Conclusion: As a conclusion, CBZ could be quantified from loaded EC microparticles using UV-Vis spectrophotometer at 286 nm. Therefore, this method can be used for the quantification analysis of CBZ in CBZ-loaded microparticles can be utilized also as an alternative method to calculate CBZ in different dosage forms.

 

Keywords: Carbamazepine, Microparticles, Method validation, UV-Vis, Spectrophotometer

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How to Cite
Mawazi, S. M., Hadi, H. A. B., Al-mahmood, S. M. A., & Doolaanea, A. A. (2019). DEVELOPMENT AND VALIDATION OF UV-VIS SPECTROSCOPIC METHOD OF ASSAY OF CARBAMAZEPINE IN MICROPARTICLES. International Journal of Applied Pharmaceutics, 11(1), 34-37. https://doi.org/10.22159/ijap.2019v11i1.26256
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