OPTIMIZATION OF GREEN SYNTHESIZED SILVER NANOPARTICLES FROM CARALLUMA UMBELLATA
Objective: The current study focuses on optimization and ecologically innocuous green synthesis of silver nanoparticles (AgNPs) using tribal plant Caralluma umbellata and to study its potential as an antibacterial and antifungal agent.
Methods: The synthesis of AgNPs were confirmed by the colour change of the stem extract from yellow to dark brown and by UV-Visible spectroscopy. The optimum conditions for synthesis of AgNPs were analysed using Response surface methodology (RSM) based Box-Behnken design (BBD) using Design Expert software (7.0.0 trial version). The AgNPs synthesized were characterized by Scanning electron microscope (SEM), Energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analysis. Further, antibacterial and antifungal activity were performed using well diffusion method for both plant extract and AgNPs.
Results: The UV-Visible spectrum of AgNPs revealed characteristic peak at 425.5 nm. The crystalline nature of synthesized AgNPs was confirmed by XRD with average size 26 nm. SEM confirms the spherical shape of AgNPs and by EDX the presence of elemental silver was observed. The ability of the plant to produce both reducing and capping agents were confirmed by FTIR. The optimum conditions for synthesis of AgNPs were found to be 0.55 mmol AgNO3 concentration, 45 Â°C temperature and 24h reaction time. Both plant sample and synthesized AgNPs exhibited good antimicrobial activity where AgNPs showed superior efficacy as an antimicrobial agent over the other.
Conclusion: From the results obtained, it can be deduced that both C. umbellata stem extract and synthesized AgNPs can act as potent antimicrobial agent. But the synthesized AgNPs is more potent against bacteria and fungus.
2. Banjare J. Application of nanotechnology in food technology and targeted drug therapy for prevention of obesity: an overview. J Crit Rev 2016;4:7-11.
3. Galib BM, Mashru M, Jagtap C, Patgiri BJ, Prajapati PK. Therapeutic potentials of metals in ancient India: a review through Charaka Samhita. J Ayurveda Integr Med 2011;2:55-63.
4. Murugesan S, Bhuvaneswari S, Sivamurugan V. Green synthesis, characterization of silver nanoparticles of a marine red alga Spyridia fusiformis and their antibacterial activity. Int J Pharm Pharm Sci 2017;9:192-7.
5. Benn T, Cavanagh B, Hristovski K, Posner JD, Westerhoff P. The realease of Nanosilver from consumer products used in the home. J Environ Qual 2010;39:1875-82.
6. Jegadeeshwari LA, Dharathi N, Lakshmi DV, Kumar EV, Gandhi NN. Tribal plants and their inborn antimicrobial activities. Asian J Pharm Clin Res 2017;10:31-7.
7. Park Y, Hong YN, Weyers A, Kim YS, Linhardt RJ. Polysaccharides and phytochemicals: a natural reservoir for the green synthesis of gold and silver nanoparticles. IET Nanobiotechnol 2011;5:69-78.
8. Chandrappa CP, Chandrasekar N, Govindappa M, Shanbhag C, Singh UK, Masarghal J. Antibacterial activity of synthesized silver nanoparticles by Simaroubaglauca against pathogenic bacteria. Int J Curr Pharm Res 2017;9:19-22.
9. Menon S, Agarwal H, Kumar SR, Kumar SV. Green synthesis of silver nanoparticles using medicinal plant Acalypha indica leaf extracts and its application as an antioxidant and antimicrobial agent against foodborne pathogens. Int J Appl Pharm 2017; 9:42-50.
10. Shah W, Patil U, Sharma A. Green synthesis of silver nanoparticles from stem bark extract of Terminalia tomentosa Roxb. (Wight and Arn.). Der Pharma Chem 2014;6:197-202.
11. Karuppusamy S, Pullaiah T. PV-01 diversity and conservation of genus Caralluma (Asclepiadaceae) in peninsular India. National Conference on Forest Biodiversity Resources: Exploitation Conservation and Management; 2006. p. 176-7.
12. Savithramma N, Yugandhar P, Devi PS, Ankanna S, Suhrulatha D, Prasad KS, et al. Documentation of ethnomedicinal information and antimicrobial validation of Thespesia populnea used by Yanadi tribe of Ganugapenta village, Chittoor district, Andhra Pradesh, India. J Int Ethnopharmacol 2017;6:158-69.
13. Jeyakumar JJ, Kamaraj M, Nandagopalan V, Anburaja V, Thiruvengadam M. A study of phytochemical constituents in Caralluma umbellata by Gc-Ms anaylsis. Int J Pharm Sci Invent 2013;2:37-41.
14. Shanmugam G, Ayyavu M, Rao DM, Devarajan T, Subramaniam G. Hepatoprotective effect of Caralluma umbellate against acetaminophen-induced oxidative stress and liver damage in rat. J Pharm Res 2013;6:342-5.
15. Bellamakondi PK, Godavarthi A, Ibrahim M. Anti-hyperglycemic activity of Caralluma umbellata Haw. BioImpacts 2014;4:113-6.
16. Balashanmugam P, Balakumaran MD, Murugan R. Phytogenic synthesis of silver nanoparticles, optimization and evaluation of in vitro antifungal activity against human and plant pathogens. Microbiol Res 2016;192:52-64.
17. Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N. Synthesis of silver nanoparticles using acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B 2010;76:50-6.
18. Coates J. Interpretation of infrared spectra, a practical approach. In: Encyclopedia of Analytical Chemistry. Meyers RA. Editor. John Wiley and Sons Ltd; Chichester; 2000.
19. Mehta BK, Chhajlani M, Shrivastava BD. Green synthesis of silver nanoparticles and their characterization by XRD. J Phys Conf Ser 2017;836:1-5.
20. Karthikeyan J, Nila KM, Thooyavan G, Vimalkumar E. Larvicidal and antibacterial efficacy of green synthesized silver nanoparticles using Melia dubia. Int J Pharm Pharm Sci 2014; 6:395-9.
21. Antony E, Sathiavelu M, Arunachalam S. Synthesis of silver nanoparticles from the medicinal plant Bauhinia Acuminata and Biophytum Sensitivumâ€“A comparative study of its biological activities with plant extract. Int J Appl Pharm 2017;9:22-9.
22. Ouay BL, Stellacci F. Antibacterial activity of silver nanoparticles: a surface science insightâ€™. Nano Today 2015; 10:339-54.
23. Hsueh YH, Lin KS, Ke WJ, Hsieh CT, Chiang CL, Tzou DY, et al. The antimicrobial properties of silver nanoparticles in Bacillus subtilis are mediated by released Ag+ion. PLoS One 2015;10:1-17.
24. Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun H, et al. Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J Proteome Res 2006;5:916-24.
25. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 2009;27:76-83.
26. Dakal TC, Kumar A, Majumdar RS, Yadav V. Mechanistic basis of antimicrobial actions of silver nanoparticles. Front Microbiol 2016;7:1-17.