• Arya G. K. Department of Pharmaceutics, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science Campus, Kochi, India
  • Anjaly A. Kumar Department of Pharmaceutics, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science Campus, Kochi, India
  • Archana J. Nair Department of Pharmaceutics, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science Campus, Kochi, India
  • Jeni Raju Department of Pharmaceutics, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science Campus, Kochi, India
  • Sreeja C Nair Department of Pharmaceutics, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science Campus, Kochi, India



Membrane Scaffolding Proteins, Phospholipid bilayer, Hydrophobic beads, High densitylipoproteins


Nano discs recently evolved as a novel tool for studying the membrane associated proteins and serve as an effective drug delivery system. Nano discs constitute disc shaped nano particles and can be defined as a membrane system which is synthetic in nature and aids in the study of membrane proteins. It is mainly made of phospholipid bilayer and the water repelling edge is isolated by amphipathic proteins called membrane scaffolding proteins [MSP]. Micelles present in the nano disc mimics the property of the biological membrane proteins. It is a powerful technology that competently delivers the drug components in to the right cells in the right tissues. Membrane scaffold proteins are primarily expressed, purified and characterized and self-assembled to form Nano discs by the process of dialysis using biobeads. Nano discs are proven to be effective in the study of membrane proteins because they can fluidize and counterbalance and also help in reclusion, refinement, biophysical and biochemical studies of them. It also presents a more genuine environment than liposomes, bicelles, amphipols and detergent micelles. Major technological advantages of nano discs include the higher stability and carrier capacity and also the increased feasibility of incorporating both hydrophilic and hydrophobic substances of drug carrier. Thus nano discs serves as an excellent system in its ability to precisely control its composition and provide a nano scale membrane surface for investigating molecular recognition events. This article reviews the emphasis of nanodiscs in studying membrane proteins as well as its effectivity in transforming into a major drug delivery system. An overview of published literatures between 1996 and 2017 was conducted to write the review.


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Brigger I, Dubernet C, Couvreur P. Nanoparticles in cancer therapy and diagnosis. Adv Drug Delivery Rev 2002;54:631–51.

Yih TC, Al-Fandi M. Engineered nanoparticles as precise drug delivery systems. J Cell Biochem 2006;97:1184-90.

Unezaki S, Maruyama K, Hosoda JI, Koyanagi Y, Nakata M, Ishida O, et al. Direct measurement of the extravasation of polyethyleneglycol-coated liposomes into solid tumor tissue by in vivo fluorescence microscopy. Int J Pharm 1996;144:11–7, 24.

Hobbs K, Monsky WL, Yuan F, Roberts WG, Griffith L, Torchilin VP, et al. Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci USA 1998;95:4607–12.

Basavaraj KH. Nanotechnology in medicine and relevance to dermatology: present concepts. Indian J Dermatol 2012;57: 169-74.

Allen TM, Cullis PR. Drug delivery systems entering the mainstream. Science 2004;303:1818-22.

Hughes G. Nanostructure-mediated drug delivery. Nanomedicine 2005;1:22–30.

Fars KA, Awwad AR, Ibrahim AA. Biopharmaceutical applications of nanogold. Saudi Pharm J 2010;18:179-93.

Wilczewska AZ, Niemirowicz K, Markjewicz KH, Car H. Nanoparticles as drug delivery systems. Pharmacol Rep 2012; 64:1020-37.

Reza K, Mohsen J. Revolutionary impact of nanodrug delivery on neuroscience. Curr Neuropharmacol 2012;1:370-92.

Singh R, Lilard JW. Nanoparticle-based targeted drug delivery. Exp Mol Pathol 2009;86:215-23.

Sarabjeet SS, Hicham F, Baljit S. Nanotechnlogy based drug delivery systems. J Occup Med Toxicol 2007;16:2-16.

Cerna T, Stiborova M, Adam V, Kizek R, Eckschlager TN. Nanocarrier drugs in the treatment of brain tumors. J Cancer Metastasis Treat 2016;2:407-16.

Koichiro T. Herbal pleiotropy-the scientific investigation may advance medicine. Int J Pharm Pharm Sci 2014;6:1-4.

Tewodros M, Ashley M, Nagesh K, Carolina S, Jinjun S, Daniel RK, et al. Emerging nanotechnology approaches for HIV/AIDS treatment and prevention. Nanomedicine 2010;5:269-85.

Sandeep S, Vivek KP, Ravi PT, Vishnu A. Nanoparticle based drug delivery system: advantages and applications. Indian J Sci Technol 2011;4:177-80.

Judith W, Paul CB, Sarah EB. Contrast agents for molecular photocaustic imaging. Nat Methods 2016;13:639-50.

Thamburu S, Shammika P, Sabitha M, Sreeja CN. Chitosan-eudragit magnetic microspheresof sulfasalazine for colon drug delivery. Int J Pharm Sci Rev Res 2016;41:125-31.

Shah H, Patel J. Bicelle: a lipid nanostructurefor transdermal delivery. J Crit Rev 2016;3:17-22.

Bayburt TH, Carlson JW, Sligar SG. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J Struct Biol 1998;123:7-44.

Hiroaki K, Keisuke I, Minoru N. Formation of size controlled, denaturation resistant lipid nanodiscs by an amphiphilic self-polymerizing peptide. Colloids Surfaces B 2016;146:423-30.

Civjan NR, Bayburt TH, Schuler MA, Sligar SG. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. Bio Techniques 2003;35:556-63.

Sunil K. Vesicular drug delivery systems: a novel approach for drug targetting. Int J Drug Delivery 2013;5:121-30.

Pandit A, Schirzad Wasei N, Wlodarczyk LM, Van Roon H, Boekema EJ, Dekker JP, et al. Assembly of major light-harvesting complex II in lipid nanodiscs. Biophys J 2011;101:2507-15.

Schuler MA, Denisov IG, Sligar SG. Nanodiscs as a new tool to examine lipid-protein interactions. Methods Mol Biol 2013;74:415-33.

Ashkan D, Valeria C, Ian WH. Self assembling amphiphilic peptides. J Pept Sci 2014;20:453-67.

William BL, David RK, Brandon VS, Nicholas AP. Polymers for drug delivery systems. Annu Rev Chem Biomol Eng 2010;1:149-73.

El Moustaine D, Granier S, Doumazane E, Scholler P, Rahmeh R, Bron P. Distinct roles of metabotropic glutamate receptor dimerization in agonist activation and G-proteincoupling. Proc Natl Acad Sci USA 2012;109:16342-7.

Mitra N, Liu Y, Liu J, Serebryani E, Mooney V, DeVree BT, et al. Calcium-dependent ligand binding and G-protein signaling of family B GPCR parathyroid hormone 1 receptor purified in nanodiscs. ACS Chem Biol 2013;8:617-25.

Bhama SK, Rakhi K, Lakshmi P, Deepa TV, Sreeja CN. Formulation and evaluation of niosomal suspension of cefixime. Asian J Pharm Clin Res 2017;10:194-201.

Borch J, Torta F, Sligar SG, Roepstorff P. Nanodiscs for immobilization of lipid bilayers and membrane receptors: kinetic analysis of cholera toxin binding to a glycolipid receptor. Anal Chem 2008;80:6245-52.

Jonas A. Reconstitution of high-density lipoproteins. Methods Enzymol 1986;128:553-82.

Silva RA, Huang R, Morris J, Gracheva EO, Ren G, Kontush A, et al. Structure of apolipoprotein A-I in spherical high density lipoproteins of different sizes. Proc Natl Acad Sci USA 2008;105:12176-81.

Gnaneswaran S, Kuberan D, Vinodhini VM, Swamy RSV, Ebenezer WW, Kumar JS. Fasting plasma glucose and glycolated haemoglobin in prediction of diabetic retinopathy in rural population. Int J Pharm Clin Res 2014;6:40-5.

Soumya S, Doney AB, Sabitha M. Current trends in lipid based delivery systems and its applications in drug delivery. Asian J Pharm Clin Res 2012;4:4-9.

Wijtske A, Uwe JFT. Regulation of reverse cholesterol transport-a comprehensive appraisal of available animal studies. Nutr Metab 2012;9:70759-25.

Astrid EV. Reverse cholesterol transport: from classical view to new insights. World J Gastroenterol 2010;16:5908-15.

Tall AR. An overview of reverse cholesterol transport. Eur Heart J 1998;19 Suppl A:A31-5.

Amy YS, Sligar SG, Schluten K. Maturation of high-density lipoproteins. J R Soc Interface 2009;6:863-71.

Vijey A, Samuel GG. Structure affinity relationship and characterization of benzoporphyrins as potent inhibitors of YAP oncoprotein in silico experiments. Int J Pharm Pharm Sci 2015;7:278-84.

Denisov IG, Sligar SG. Nanodiscs for structural and functional study of membrane proteins. Nat Struct Mol Biol 2016;23:481-6.

Borch J, Hamann T. The nanodisc: a novel tool for membrane protein studies. Biol Chem 2009;390:805-14.

Hagn F, Etzkorn M, Raschle T, Wagner G. Optimized phospholipid bilayer nanodiscs facilitate high resolution structure determination of membrane proteins. J Am Chem Soc 2013;135:1919-25.

Rouck JE, Krapf JE, Roy G, Huff HC, Das A. Recent advances in nanodisc technology for membrane protein studies. FEBS Lett 2017;591:2057-88.

Bayburt TH, Sligar SG. Membrane protein assembly into nanodiscs. FEBS Lett 2010;58:1721-7.

Shefrin S, Sreelaxmi CS, Vishnu V, Sreeja CN. Enzymosomes: a rising effectual tool for targeted drug delivery system. Int J Appl Pharm 2017;9:1-9.

Denisov IG, Sligar SG. Cytochromes p450 in nanodiscs. Biochim Biophys Acta 2011;1814:223-9.

Ritchie TK, Grinkova YV, Bayburt TH, Denisov IG, Zolnerciks JK, Atkins WM, et al. Reconstitution of membrane proteins in phospholipid bilayer nanodiscs. Methods Enzymol 2009;464:211-31.

Bayburt TH, Sligar SG. Membrane protein assembly into nanodiscs. FEBS Lett 2010;584:1721-7.

Nath A, Atkins WM, Sligar SG. Applications of phospholipid bilayer nanodiscs in the study of membranes and membrane proteins. Biochemistry 2007;46:2059-69.

Denisov IG, McLean MA, Shaw AW, Grinkova YV, Sligar SG. Thermotropic phase transition in soluble nanoscale lipid bilayers. J Phys Chem B 2005;109:15580-8.

Banerjee S, Huber T, Sakmar T. Rapid incorporation of functional rhodopsin into nanoscale apo lipoprotein bound bilayer particles. J Mol Biol 2008;377:1067-81.

Thenesh SK, Oshin S, Sudharsan CR. Efficacy of hydrotropes on the solubility of forskolin in water. Int J Appl Pharm 2016;8:1-4.

Kunjan P, Marika N, Rupanjali BS, Hemanta KS. Nanosized drug delivery systems for direct nose to brain targeting: a review. Recent Pat Drug Delivery Formul 2016;10:156-64.

Denisov IG, Grinkova YV, Lazarides AA, Sligar SG. Directed self-assembly of monodisperse phospholipid bilayer nanodiscs with controlled size. J Am Chem Soc 2004;126:3477-87.

Bayburt TH, Grinkova YV, Sligar SG. Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins. Nanoletters 2002;2:853-6.

Dhanalakshmi V, Nimal TR, Sabitha M, Biswas R, Jayakumar R. Skin and muscle permeating antibacterial nanoparticles for treating staphylococcus aureus infected wounds. J Biomed Mater Res Part B Appl Biomater 2016;1:797-807.

Serebryany, Zhu GA, Yan YC. Artificial membrane-like environments for in vitro studies of purified G-protein coupled receptors. Biochem Biophys Acta 2012;1818:225-33.

Sligar SG. Finding a single-molecule solution for membrane proteins. Biochem Biophys Res Comm 2003;312:115-9.

Jayakumar R. Biological macromolecules for tissue regeneration. Int J Biol Macromol 2016;93:1337.

Glueck JM, Koenig BW, Wilbold D. Nanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies. Anal Biochem 2011;408:46-52.

Kuai R. Designer vaccine nanodisc for personalised cancer immunotherapy. Nat Mater 2016;16:489-96.

Karasaki T, Nakajima J, Kakimi K. Neoantigens and whole-exome sequencing. Gan Kaqaku Ryoho 2016;43:791-7.

Hirayama M, Nishimur Y. The present status and future prospects of peptide-based cancer vaccines. Int Immunol 2016;28:319-28.

Desrichard A, Synder A, Chan TA. Cancer neoantigens and applications for immunotherapy. Clin Cancer Res 2016;22:807-12.

Glueck JM, Wittlich M, Feuerstein S, Hoffmann S, Willbold D, Koeniq BW. Integral membrane proteins in nanodiscs can be studied by solution NMR spectroscopy. J Am Chem Soc 2009;131:12060-1.

Xu Z, Wang Y, Zhang L, Huang L. Nanoparticle-delivered transforming growth factor-beta siRNA enhances vaccination against advanced melanoma by modifying tumor microenvironment. ACS Nano 2014;8:3636–45.

Zhanq X, Sharma PK, Peter Goedegebuure S, Gillanders WE. Personalised cancer vaccines targeting the cancer mutanom. Vaccine 2017;35:1094-100.

Hagn F, Etzkorn M, Raschle T, Wagner G. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J Am Chem Soc 2013;135:1919-25.

Iacono P, Battaglia PM, Falcomata B, Bandello F. Central serous chorioretinopathy treatments: a mini review. Ophthalmic Res 2016;55:76-83.

Aswathy SN, Vidya KM, Saranya TR, Sreeja CN. Emulsomes: a novel liposomal formulation for sustained drug delivery. Int Res J Pharm Appl Sci 2013;3:192-6.

Shen HM, Zhang QF. Risk assessment of nickel carcinogenicity and occupational lung cancer. Environ Health Perspect 1994;102 Suppl 1:275-82.

Payne DK, Sullivan MD, Massie MJ. Women’s psychological reactions to breast cancer. Semin Oncol 1996;23(1, Suppl 2):89-97.

Januz B, Joanna P, Joanna Z. Nanoparticles of titanium and zinc oxides as novel agent in tumor treatment: a review. Nanoscale Res Lett 2017;12:1-15.

Atyabi F, Sharma HL, Mohammad HAH, Fell JT. Controlled drug release from coated floating ion exchange resin beads. J Controlled Release 1996;42:25-8.



How to Cite

K., A. G., Kumar, A. A., Nair, A. J., Raju, J., & Nair, S. C. (2019). NANODISCS: A NEW EPOCH IN THE STUDY OF MEMBRANE PROTEINS AND AS AN EMERGING DRUG DELIVERY SYSTEM. International Journal of Applied Pharmaceutics, 11(1), 1–6.



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