Siva Kumar Ramamurthy, Chenchugari Sridhar, Chenchugari Sridhar


Objective:     To biosynthesize zinc oxide nanoparticles by using parthenium hysterophorous plant extract as a reducing agent and its characterization by spectroscopic techniques.

Methods:      A novel method was developed to prepare zinc oxide nanoparticles by using zinc    nitrate as a precursor and biosynthesis of zinc oxide nanoparticles was mediated by parthenium hysterophorous plant extract without the aid of external energy (high pressure and temperature). This new method involves simple techniques such as centrifugation, filtration and stirring. Zinc oxide nanoparticles formation was confirmed by analytical techniques such as UV-Visible spectroscopy, powder X-ray diffraction (XRD), Raman spectroscopy and by scanning electron microscopy (SEM) analysis.

Results:         Zinc oxide nanoparticles were synthesized by using parthenium hysterophorous plant extract as a reducing agent. The XRD measurement showed that zinc oxide nanoparticles possess a typical hexagonal structure and the crystallite size of the synthesized zinc oxide nanoparticles was found to be 32 nm calculated by scherrer’s formula. The SEM images show agglomeration of zinc oxide nanoparticles that are spherical clusters. The maximum absorbance (380nm) of UV-Visible spectroscopy further confirmed synthesized nanoparticles are zinc oxide. The Raman spectra show both E2 mode and E1 mode, which indicates that the prepared zinc oxide nanoparticles possess crystalline nature with hexagonal wurtzite structure.

Conclusion:    A method was established to prepare zinc oxide nanoparticles with parthenium hysterophorous plant extract which is a novel approach without the aid of external energy (high pressure and temperature) and formation of zinc oxide nanoparticles was confirmed by spectroscopic techniques. This method can be used in pharmaceutical            industry for the synthesis of an antimicrobial agent. 


Zinc oxide, Nanoparticles, Parthenium hysterophorous, Raman spectra


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