EXPLORING PROPERTIES OF SWEET BASIL SEED MUCILAGE IN DEVELOPMENT OF PHARMACEUTICAL SUSPENSIONS AND SURFACTANT-FREE STABLE EMULSIONS
Objective: The objective of the investigation was to isolate mucilage from sweet basil seeds and explore its physicochemical properties for the development of pharmaceutical suspensions and surfactant-free stable emulsions.
Methods: Possible applications of sweet basil seed mucilage in the pharmaceutical field for dosage form development are being explored. The physicochemical and functional properties of the mucilage from the seeds of the Ocimum basilicum L. (Sweet basil) have been investigated for stabilization of suspensions and emulsions. The following analyses were performed: FTIR spectroscopy, phytochemical tests, XRD, swelling and rheological studies.
Results: The analyses showed that the mucilage is rich in glucose, mannose, and xylose. High swelling index values varying from 100Â±10 to 200Â±13%, high water-holding capacity of 97.5Â±2.4 g/g mucilage and reasonable oil holding capacity of the mucilage (13.2Â±1.3 g/g mucilage) makes it an ideal candidate for utilization as viscosifier and stabilizer of suspensions and surfactant-free emulsions. Adult and paediatric paracetamol suspension formulations with 1%w/v mucilage have exhibited flocculated nature and good stability owing to its high sedimentation volume(F= 0.85-0.98) and good redispersibility. Sunflower oil emulsions prepared with 0.25%w/v mucilage demonstrated emulsion stability index of 105.714 on 5th day and extremely low creaming rate of 0.0004 cm/h thus confirming maximum stability compared to emulsions developed with 0.3-0.5% w/v mucilage.
Conclusion: The mucilage isolated from Ocimum basilicum L. seeds may be regarded as a functional biomaterial for pharmaceutical use to ensure quality and stability of liquid dosage forms.
2. Saeedi M. Evaluation of binding properties of Plantago psyllium seed mucilage. Acta Pharm 2010;60:339â€“48.
3. Munoz LA, Cobos A, Diaz O, Aguilera JM. Chia seeds: microstructure, mucilage extraction, and hydration. J Food Eng 2012;108:216-24.
4. Bakre LG, Ajakore O. Suspending properties of natural gums extracted from Abelmuscus esculentus pod and Chrysophyllum albidium fruit. Afr J Pharm Phamcol 2015;9:321-6.
5. Surfactant-free emulsions and polymer-stabilization of emulsions used in skin care products. Azonano; 2005. Available from: https://www.azonano.com/article.aspx?ArticleID=1292. [Last accessed on 18 Feb 2018].
6. Stabilised surfactant-free emulsion; 2013. Available from: http://www.inra.fr/en/Partners-and-Agribusiness/Results-Innovations-Transfer/All-the-news/Stabilised-surfactant-free emulsions. [Last accessed 16 Feb 2018].
7. Kumpugdee Vollrath M, Krause JP, Burk S. Surfactant-free o/w-emulsion as drug delivery system. Int J Pharmacol Pharm Sci 2014;8:574-7.
8. Werner A, Schmitt V, Sebe G, Heroguez H. Synthesis of surfactant-free micro-and nanolatexes from pickering emulsions stabilized by acetylated cellulose nanocrystals. Polym Chem 2017;8:6064-72.
9. Khan BA, Akhtar N, Khan HMS, Waseem K, Mahmood T, Rasul A, et al. Basics of pharmaceutical emulsions: a review. Afr J Pharm Pharmacol 2011;5:2715-25.
10. Tadros TF. Emulsion formation, stability, and rheology in Emulsion Formation and Stability. First Edition. Edited by Tharwat F. Tadros. Wiley-VCH Verlag GmbH and Co. KGaA; 2013.
11. Kadam PV, Yadav KN, Jagdale SK, Shivatare RS, Bhilwade SK, Patil MJ. Evaluation of Ocimum sanctum and Ocimum basilicum mucilage-As a pharmaceutical excipient. J Chem Pharm Res 2012;4:1950-5.
12. Bekers AGM, Kroh A. Carbohydrate composition of the mucilage on Ocimum basilicum L. seeds. Acta Bot Neerl 1978;27:121-3.
13. Nazir S, Wani I, Masoodi FA. Extraction optimization of mucilage from basil (Ocimum basilicum L.) seeds using response surface methodology. J Adv Res 2017;8:235â€“44.
14. Akbari I, Ghoreishi SM. Generation of porous structure from basil seed mucilage via supercritical fluid assisted process for biomedical applications. Int J Pharm Sci Dev Res 2017;3:30-5.
15. Saeedi M, Morteza Semnani K, Akbari J, Bazargani MH, Amin G. Evaluation of Ocimum basilicum L. seed mucilage as rate controlling matrix for sustained release of propranolol HCl. Pharm Biomed Res 2015;1:18-25.
16. AACC, Approved methods of the american association of cereal chemists, American Association of cereal chemists, St. Paul, Minn, USA; 1984.
17. Campos MRS, Solis NC, Rubio GR, Chel-Guerrero L, Betancur-Ancona D. Chemical and functional properties of chia seed gum. Int J Food Sci 2014. http://dx.doi.org/10.1155/2014/241053.
18. Timilsena YP, Adhikari R, Kasapis S, Adhikari B. Molecular and functional characteristics of purified gum from Australian chia seeds. Carb Poly 2015. http://dx.doi.org/doi:10.1016/ j.carbpol.2015.09.035
19. Kassakul W, Praznik W, Viernstein H, Phrutivorapongkul, Leelapornpisid. Characterization of the mucilages extracted from Hibiscus rosa-sinensis Linn and Hibiscus mutabilis Linn and their skin moisturizing effect. Int J Pharm Pharm Sci 2014;6:453-7.
20. Sharma A, Jain CO. Preparation and characterisation of solid dispersions of carvedilol with PVP K30. Res Pharm Sci 2010;5:49-56.
21. The density of cooking oil. Available from: https://hypertextbook.com/facts/2000/IngaDorfman.shtml. [Last accessed on 20 Jan 2018].
22. Zameni A, Kashaninejad M, Aalami M, Salehi F. Effect of thermal and freezing treatments on rheological, textural and color properties of basil seed gum. J Food Sci Technol 2015;52:5914â€“21.
23. Deshmukh SS, Katare YS, Shyale SS, Bhujbal SS, Kadam SD, Landge DA, et al. Isolation and evaluation of mucilage of Adansonia digitata Linn as a suspending agent. J Pharm 2013:1-4. http://dx.doi.org/10.1155/2013/379750
24. Samuel NG, Gebre Mariam T. Evaluation of the suspending properties of two local Opuntia spp. mucilages on paracetamol suspension. Pak J Pharm Sci 2010;26:23-9.
25. Bamiro OA, Ajala TO, Adenokun EG. A new emulsifying agent: Cucumis sativus L. mucilage. J Pharm Res Int 2017;17:1-9.
26. Goh KKT, Merino L, Chiang JH, Quek R, Soh SJB, Lentle RG. The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology. Carb Polymers 2016;149:297â€“307.
27. Monrroy M, Garcia E, Rios K, Garcia JR. Extraction and physicochemical characterization of mucilage from Opuntia cochenillifera (L.) Miller. J Chem 2017. https://doi.org/ 10.1155/2017/4301901.
28. Davidson GR, Peppas NA, Solute and penetrant diffusion in swellable polymers: relaxation-controlled transport in P (HEMA-co-MMA) copolymers. J Contr Rel 1986;3:243-58.
29. Farahnaky A, Aksari H, Majzoobi M, Mesbahi GH. The impact on concentration, temperature, and pH on dynamic rheology of psyllium gels. J Food Eng 2010;100:294-301.
30. Morris ER. Shear-thinning of â€˜random coilâ€™ polysaccharides: Characterization by two parameters from a simple linear plot. Carbohydr Polym 1990;13:85â€“96.
31. Casas JA, Mohedano AF, GarcÄ±a Ochoa F. Viscosity of guar gum and xanthan/guar gum mixture solutions. J Sci Food Agric 2000;80:1722-7.
32. Munzel K, Gecgil S. The influence of additives and different procedures on the viscosity of tragacanth mucilages. J Fac Pharm 1977;7:42-9.
33. Aremu OI, Oduyela OO. Evaluation of metronidazole suspensions. Afr J Pharm Pharmacol 2015;9:439-50.
34. Nayak AK, Pal D, Pany DR, Mohanty B. Evaluation of Spinacia oleracea l. leaves mucilage as an innovative suspending agent. J Adv Pharm Tech Res 2010;1:338-41.
35. Chikkappaiah L, Harish Nayaka MA, Mahadeviah, Prashanth Kunar GM. Preparation of plant mucilage clarificants and their effect on jiggery processing of sugarcane variety Co 86032. Int J Pharm Pharm Sci 2017;9:32-6.
36. Mahfoudhi N, Chouaibi M, Donsi F, Ferrari G, Hamdi S. Chemical composition and functional properties of gum exudates from the trunk of the almond tree. Food Sci Tech Int 2012;18:241-50.
37. Andrade LA, Nunes CA, Pereira J. Relationship between the chemical components of taro rhizome mucilage and its emulsifying property. Food Chem 2015;178:331-8.