• A ANTONY LAWRENCE PG and Research Department of Physics, Government Arts College, (Affiliated to Bharathidasan University), Trichy 620022
  • J THOMAS JOSEPH PRAKASH PG and Research Department of Physics, Government Arts College, (Affiliated to Bharathidasan University), Trichy 620022


Objective: The present study was to synthesize nanoparticles using Manilkara hexandra stem bark extract its characterization and evaluating it by an antimicrobial and antioxidant assay.

Methods: Manilkara hexandra stem bark silver nanoparticles (MHSB-AgNPs) was done by mixing silver nitrate (1 mmol) and aqueous stem bark extract and it was analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, Field Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive Spectroscopy (EDAX), Thermogravimetry/Differential Thermal Analysis (TG/DTA) and Differential scanning calorimetry (DSC). The antibacterial assay was done by a well diffusion method and also examined for antifungal assay was done by disk diffusion method and antioxidant potential Diphenyl-1-picryl hydrazyl (DPPH method)

Results: Manilkara hexandra stem bark silver nanoparticles (MHSB-AgNPs) is characterized by various techniques such as UV-visible absorption spectrum ranges from 430 nm to 440 nm indicate silver nanoparticles. The Fourier Transform Infrared Spectroscopy consists of biomolecules acts as capping agent to form silver nanoparticles. Field Emission Scanning Electron Microscopy shows particle size ranges from 15 nm to 50 nm. Energy Dispersive Spectroscopy shows the presence of Silver. X-ray Diffraction corresponds to face-centered lattice planes (111), (200), (220) and (311). Dynamic Light Scattering show the range of 68 nm and Zeta potential show the negative value of-17 nm which has high stability. Silver nanoparticles is also examined by Thermogravimetry/Differential Thermal Analysis (TG/DTA) and Differential scanning calorimetry (DSC) this project the thermal stability of the nanoparticles. The aqueous stem bark is also examined by UV-visible absorption spectrum, Fourier Transform Infrared Spectroscopy (FTIR), and Gas Chromatography-Mass Spectrometry (GCMS). In GCMS 20 compounds were identified. Silver nanoparticles show high zone of inhibition in antimicrobial assays and act as a good antioxidant agent.

Conclusion: It is eco-friendly, non-toxic, and it’s easy to synthesis and it shows good result in an antimicrobial and antioxidant assay can be applied in a pharmaceutical application.

Keywords: Manilkara hexandra, Stem bark extract, Silver nanoparticles, Antibacterial assays, Antioxidant assay


1. Valiathan MS. Healing plants. Curr Sci 1998;75:1122-6.
2. Robinson MM, Zhang X. Traditional medicines: global situation, issues and challenges, the world medicines situation. 3rd ed. World Health Organization, Geneva; 2011.
3. Alexander JW. History of the medical use of silver. Surg Infect 2009;10:289-92.
4. Rai M, Yadav A. Plants as potential synthesiser of precious metal nanoparticles: progress and prospects. IET Nanobiotechnol 2013;7:117-24.
5. Anuradha G, Syama Sundar B, Ramana MV, Sreekanth Kumar J, Sujatha T. Single-step synthesis and characterization of silver nanoparticles from Ocimum tenuiflorum L. green and purple. IOSR J Appl Chem 2014;7:123-7.
6. Jayapriya E, Lalitha P. Synthesis of silver nanoparticles using leaf aqueous extract of Ocimum basilicum (L.). Int J Chem Tech Res 2013;5:2985-92.
7. Patra JK, Baek KH. Green nanobiotechnology: factors affecting synthesis and characterization techniques. J Nanomat 2014;2014:1-12.
8. Eby DM, Luckarift HR, Johnson GR. Hybrid antimicrobial enzyme and silver nanoparticles coatings for medical instruments. ACS Appl Mater Interfaces 2009;1:1553-60.
9. Narayanan KB, Sakthivel N. Green synthesis of biogenic metal nanoparticles by terrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatible agents. Adv Colloid Interface Sci 2011;169:59-79.
10. Irudaya Monisha S, Jeyaleela GD, Immaculate AA, Vimala JR. Comparative studies on yield and the phytochemical appraisal (Quality and Quantity) of Manilkara hexandra (Roxb) dubard using leaf, stem, and bark. J Pharmacogn Phytother 2017;6:2052-8.
11. Tripathi V, Tabor SL, Mantica PF, Utsuno Y, Bender P, Cook J, et al. Physical Rev Lett 2010;104:129202.
12. Menon S, Agarwal H, Kumar RS, Venkat Kumar S. Green synthesis of silver nanoparticles using medicinal plant Acalypha indica leaf extracts and its application as an antioxidant and antimicrobial agent against foodborne pathogens. Int J Appl Pharm 2017;9:42-50.
13. Harborne JB. Textbook of phytochemical methods. 1st ed. London: Chapman and Hall; 1973.
14. Wright GD. Resisting resistance: new chemical strategies for battling superbugs. Chem Biol 2000;7:R127-32.
15. Bhattacharya D, Rajinder G. Nanotechnology and potential of microorganisms. Crit Rev Biotechnol 2005;25:199-204.
16. Mohamed S Abdel Aziz, Mohamed S Shaheen, Aziza A El-Nekeety, Mosaad A Abdel Wahhab. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. J Saudi Chem Soc 2014;18:356-63.
17. Anthony E, Sathiavelu M, Arunachalam S. Synthesis of silver nanoparticles from the medicinal plant Bauhinia acuminata and Biophytum sensitivum a compartitive study of its biological activities with plant extract. Int J Appl Pharm 2017;9:22-9.
18. Marie Isabelle Baraton. Surface analysis of semiconducting nanoparticles by FTIR spectroscopy, nanocrystalline metals and oxides, springer, boston, MA; 2002. p. 165-87.
19. Shameli K, Ahmad MB, Al-Mulla EAJ, Ibrahim NA, Shabanzadeh P, Rustaiyan A, et al. Green biosynthesis of silver nanoparticles using callicarpa maingayi stem bark extraction. Molecules 2012;17:8506-17.
20. Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, et al. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B 2010;81:81-6.
21. Zhang Y, Kohler N, Zhang M. Surface modification of superparamagnetic magnetite nanoparticles and their intracellular uptake. Biomaterials 2002;23:1553-61.
22. Khan MAM, Kumar S, Ahamed M, Alrokayan SA, AlSalhi MS. Structural and thermal studies of silver nanoparticles and electrical transport study of their thin films. Nanoscale Res Lett 2011;6:434.
23. Romero CD, Chopin SF, Buck G, Martinez E, Garcia M, Bixby L. Antibacterial properties of common herbal remedies of the southwest. J Ethnopharmacol 2005;99:253-7.
24. Farukh A, Ahmad I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish J Biol 2006;30:177-83.
25. Bhumi G, Rao G, Savithramma N. Green synthesis of silver nanoparticles from the leaf extract of Adhatoda vasica nees and assessment of its antibacterial activity. Asian J Pharm Clin Res 2015;8:42-50.
26. Patel S, Sivaraj R, Rajiv P, Venckatesh R, Seenivasan R. Green synthesis of silver nanoparticles from the leaf extract of Aegle marmelos and evaluation of its antibacterial activity. Int J Pharm Pharm Sci 2015;7:169-73.
27. Fatimah Is, Indrani N. Silver nanoparticles synthesized using Lantana camara flower extract by reflux, microwave and ultrasound methods. Chem J Moldova 2018;13:1857-27.
28. Shabanzadeh P, Yusof R, Shameli K. Artificial neural network for modeling the size of silver nanoparticles’ prepared in montmorillonite/starch bionanocomposites. J Industrial Eng Chem 2015;24:42-50.
29. Valodkar M, Nagar PS, Jadeja RN, Thounaojam MC, Devkar RV, Thakore S. Euphorbiaceae latex-induced green synthesis of noncytotoxic metallic nanoparticle solutions: a rational approach to antimicrobial applications. Colloids Surf A 2011;384:337-44.
30. Haqq SM, Pandey H, Gerard M, Chattree A. Bio-fabrication of silver nanoparticles using Chrysanthemum coronarium flower extract and It’s in vitro antibacterial activity. Int J Appl Pharm 2018;10:209-13.
31. Anju K, Jegadeeshwari AL, Gandhi NN. Optimization of green synthesized silver nanoparticles from Caralluma umbellata. Int J Appl Pharm 2018;10:103-10.
32. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri BJ, Ramrez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology 2005;16:2346–53.
33. Song HY, Ko KK, Oh IH, Lee BT. Fabrication of silver nanoparticles and their antimicrobial mechanisms. Eur Cells Mater 2006;11:58.
34. Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 2000;52:662-8.
35. Wu D, Cederbaum AI. Alcohol,oxidative stress, and free radical damage. Alcohol Res Health 2003;27:277-84.
36. Priya RS, Geetha D, Ramesh PS. Antioxidant activity of chemically synthesized AgNPs and biosynthesized Pongamia pinnata leaf extract mediated AgNPs a comparative study. Ecotoxicol Environ Saf 2016;134:308-18.
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