FORMULATION AND DISSOLUTION PROFILE STUDY OF TRANSFERSOME-LOADED MICROSPHERES FROM GREEN TEA LEAF EXTRACT

Authors

  • Effionora Anwar Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Putri Amalia Handayani Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2018.v10s1.47

Keywords:

Dissolution profile study, Epigallocatechin gallate, Green tea leaf extract, Microsphere, Transfersome

Abstract

Objective: The aim of this study was to prepare transfersome-loaded microspheres which had good characteristics and physicochemical stability to
increase bioavailability of the polyphenol component of green tea leaf extract in the body.
Methods: Transfersomes were prepared using a thin-layer hydration method. Green tea leaf extract transfersomes were formulated in the ratio of
95:5, 90:10, and 85:15 based on their phospholipid and Span 80 content.
Results: The most successful formula produced transfersomes of a spherical shape, 78.75 nm in size with a polydispersity index of 0.187, zeta
potential of −37.5 mV, and entrapment efficiency of 47.96±5.81%. Subsequently, the transfersome was loaded into a microsphere using the spray-dry
method. The microspheres had a non-spherical, wrinkled shape, their size was 2058.44 nm, their polydispersity index was 0.545, their entrapment
efficiency was 59.27±0.59%, their moisture content was 5.21%, and their swelling index was 289.36% after 4 h. The total cumulative amount of
Epigallocatechin-3-gallate after a dissolution test was 69.15±7.66%.
Conclusion: The physicochemical stability of transfersome-loaded microspheres was not significantly different from that of transfersome powder.

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References

Chang K. World Tea Production and Trade: Current and Future

Development. Rome, Italy: Food and Agriculture Organization of the

United Nations; 2015.

Graham HN. Green tea composition, consumption, and polyphenol

chemistry. Prev Med 1992;21:334-50.

Anand J, Gautam P, Rai N. Comparative study of antibacterial and

anti-proliferative potential of green tea from different geographical

locations in India. Asian J Pharm Clin Res 2015;8:253-8.

Coppock R, Dziwenka M. Green Tea Extract. Nutraceuticals. London:

Elsevier; 2016. p. 633-52.

Mohan M, Jeevanandan G, Mithun RS. The role of green tea in oral

health-a review. Asian J Pharm Clin Res 2018;11:1-3.

Zokti JA, Baharin BS, Mohammed AS, Abas F. Green tea leaves

extract: Microencapsulation, physicochemical and storage stability

study. Molecules 2016;21:1-24.

Saraf AS. Applications of novel drug delivery system for herbal

formulations. Fitoterapia 2010;81:680-9.

Henning SM, Niu Y, Lee NH, Thames GD, Minutti RR, Wang H,

et al. Bioavailability and antioxidant activity of tea flavanols after

consumption of green tea, black tea, or a green tea extract supplement.

Am J Clin Nutr 2004;80:1558-64.

Anwar E, Utami TD, Ramadon D. Transfersomal gel containing green

tea (Camellia sinensis L. Kuntze) leaves extract: Increasing in vitro

penetration. Asian J Pharm Clin Res 2017;10:294-8.

Shilakari G, Singh D, Asthana A. Novel vesicular carriers for topical

drug delivery and their application’s. Int J Pharm Sci Rev Res

;21:77-86.

Zhang J, Tang Q, Xu X, Li N. Development and evaluation of a novel

phytosome-loaded chitosan microsphere system for curcumin delivery.

Int J Pharm 2013;448:168-74.

Fangueiro JF, Parra A, Silva AM, Egea MA, Souto EB, Garcia ML, et al.

Validation of a high performance liquid chromatography method for the

stabilization of epigallocatechin gallate. Int J Pharm 2014;475:181-90.

Hanani E, Munim A, Sekarini R. Identification of antioxidant

compounds in Callyspongia sp from Seribu Island. Majalah llmu

Kefarmasian 2005;2:127-33.

Rajan R, Vasudevan DT. Effect of permeation enhancers on the

penetration mechanism of transfersomal gel of ketoconazole. J Adv

Pharm Technol Res 2012;3:112-6.

Rocha S, Generalov R, Pereira Mdo C, Peres I, Juzenas P, Coelho MA,

et al. Epigallocatechin gallate-loaded polysaccharide nanoparticles for

prostate cancer chemoprevention. Nanomedicine (Lond) 2011;6:79-87.

Yu F, Li Y, Chen Q, He Y, Wang H, Yang L, et al. Monodisperse

microparticles loaded with the self-assembled berberine-phospholipid

complex-based phytosomes for improving oral bioavailability

and enhancing hypoglycemic efficiency. Eur J Pharm Biopharm

;103:136-48.

Harmita S. Physicochemistry. Depok: Department of Pharmacy,

Universitas Indonesia; 2006.

Dolan JW. System suitability. LCGC Eur 2004;17:1-4.

Mishra K, Ojha H, Chaudhury NK. Estimation of antiradical properties

of antioxidants using DPPH assay: A critical review and results. Food

Chem 2012;130:1036-43.

Harbowy ME, Balentine DA, Davies AP, Cai Y. Tea chemistry. Crit Rev

Plant Sci 1997;16:415-80.

El Zaafarany GM, Awad GA, Holayel SM, Mortada ND. Role of edge

activators and surface charge in developing ultradeformable vesicles

with enhanced skin delivery. Int J Pharm 2010;397:164-72.

Jain S, Jain P, Umamaheshwari RB, Jain NK. Transfersomes-a novel

vesicular carrier for enhanced transdermal delivery: development,

characterization, and performance evaluation. Drug Dev Ind Pharm

;29:1013-26.

Malvern Instruments, Ltd. Zetasizer Nano: User Manual. Worcestershire,

United Kingdom: Malvern Instruments, Ltd.; 2013.

Arizka AA, Daryatmo J. Changes in moisture and tea water content

during storage at different temperatures and packaging. Apl Teknol

Pangan J 2015;4:124-9.

Published

20-12-2018

How to Cite

Anwar, E., & Handayani, P. A. (2018). FORMULATION AND DISSOLUTION PROFILE STUDY OF TRANSFERSOME-LOADED MICROSPHERES FROM GREEN TEA LEAF EXTRACT. International Journal of Applied Pharmaceutics, 10(1), 211–215. https://doi.org/10.22159/ijap.2018.v10s1.47

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Original Article(s)