AN INNOVATIVE WAY FOR EXTRACTION OF ESSENTIAL OIL COMPONENTS FROM PIMPINELLA ANISUM L. FRUITS

  • Nikolay N. Boyko Laboratory of technology of drugs, Belgorod National Research University, 85, Pobedy St., Belgorod, Russia, 308015
  • Dmitriy I. Pisarev Department of General chemistry,Belgorod National Research University, 85, Pobedy St., Belgorod, Russia, 308015
  • Elena T. Zhilyakova Department of Pharmaceutical Technology, Belgorod National Research University, 85, Pobedy St., Belgorod, Russia, 308015.
  • Anastasiya Yu. Malyutina Department of General chemistry, Belgorod National Research University, 85, Pobedy St., Belgorod, Russia, 308015.
  • Oleg O. Novikov Test Department "Drugs quality control center" of the Shared Research and Education Center, Рeoples’ Friendship University of Russia, 8/2, Miklukho-Maklay St., Moscow, Russia, 117198
  • Victoria Yu. Kuznietsova Department of Chemistry of Natural Compounds, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.
  • Rita V. Sahaidak-Nikitiuk Department of Processes and Apparatuses of Chemical and Pharmaceutical Industries, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.
  • Mykola Ye. Blazheyevskiy Department of Physical and Colloid Chemistry, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.
  • Oleg S. Shpychak Department of Industrial Pharmacy and Economy Institute for Advanced Training of Pharmacy Specialists, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.
  • Natalia A. Sushchuk Department of Technology of Drugs, Odessa National Medical University, 2, Valikhovsky lane, Odessa, Ukraine, 65000.

Abstract

Objective: The aim of this article was to test the hypothesis about the possibility for extraction of essential oil components from the plant raw material (PRM) by the example of Pimpinella anisum L. fruits with two different perfluoro organic solvents, Novec 1230 and Novec 7100.


Methods: For the studies, we used Pimpinella anisum L. fruits ground to obtain particle size between 0.1 and 0.5 mm. The study of qualitative and quantitative content of the extracts was carried out by the following method: simple maceration, PRM: extractant ratio 1:10 w/v; and circulation method for extraction using Soxhlet extractor, PRM: extractant ratio 1:5 w/v. Qualitative analysis of extracts was carried out by gas chromatography-mass spectrometry (GC-MS) method. Quantitative analysis of trans-anethole in the extracts was carried out by reverse phase high-performance liquid chromatography (RP HPLC) method.


Results: The largest yield of anethole was observed for n-hexane, 84±4 %, and for Novec 7100, 81±4 %. In case of Novec 1230 solvent, the yield of anethole was noted to be three times less, 32±2 %. The yield of anethole under the conditions of solvents (Novec 1230 and Novec 7100) circulation method was up to 94±5 % within two hours.


Conclusion: Adequacy of the working hypothesis about the possibility for extraction of essential oil components from Pimpinella anisum L. fruits by two different perfluoro organic solvents, Novec 1230 and Novec 7100, was verified experimentally. In the context of the solvents tested, it was found that the level of fluorination of the solvent’s molecule had a great influence on its extraction properties in regard to anethole and vegetable oil.

Keywords: RP HPLC, GC-MS, essential oil, anethole, Pimpinella anisum L., Novec 1230, Novec 7100, n-hexane

Author Biographies

Nikolay N. Boyko, Laboratory of technology of drugs, Belgorod National Research University, 85, Pobedy St., Belgorod, Russia, 308015

Laboratory of technology of drugs

Dmitriy I. Pisarev, Department of General chemistry,Belgorod National Research University, 85, Pobedy St., Belgorod, Russia, 308015

Department of General chemistry

Elena T. Zhilyakova, Department of Pharmaceutical Technology, Belgorod National Research University, 85, Pobedy St., Belgorod, Russia, 308015.

Department of Pharmaceutical Technology, Doct.Pharm.Sc., professor

Anastasiya Yu. Malyutina, Department of General chemistry, Belgorod National Research University, 85, Pobedy St., Belgorod, Russia, 308015.

Associate Professor of General Chemistry Department

Oleg O. Novikov, Test Department "Drugs quality control center" of the Shared Research and Education Center, Рeoples’ Friendship University of Russia, 8/2, Miklukho-Maklay St., Moscow, Russia, 117198

Test Department "Drugs quality control center" of the Shared Research and Education Center, doct.pharm.sc., professor

Victoria Yu. Kuznietsova, Department of Chemistry of Natural Compounds, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.

Department of Chemistry of Natural Compounds, cand.pharm.sc., associate professor

Rita V. Sahaidak-Nikitiuk, Department of Processes and Apparatuses of Chemical and Pharmaceutical Industries, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.

Department of Processes and Apparatuses of Chemical and Pharmaceutical Industries, doct.pharm.sc., professor

Mykola Ye. Blazheyevskiy, Department of Physical and Colloid Chemistry, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.

Department of Physical and Colloid Chemistry, doct.chem.sc., professor

Oleg S. Shpychak, Department of Industrial Pharmacy and Economy Institute for Advanced Training of Pharmacy Specialists, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.

Department of Industrial Pharmacy and Economy Institute for Advanced Training of Pharmacy Specialists, National University of Pharmacy, 53, Pushkinska St., Kharkiv, Ukraine, 61002.

Natalia A. Sushchuk, Department of Technology of Drugs, Odessa National Medical University, 2, Valikhovsky lane, Odessa, Ukraine, 65000.

Department of Technology of Drugs, associate professor

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Boyko, N. N., Pisarev, D. I., Zhilyakova, E. T., Malyutina, A. Y., Novikov, O. O., Kuznietsova, V. Y., Sahaidak-Nikitiuk, R. V., Blazheyevskiy, M. Y., Shpychak, O. S., & Sushchuk, N. A. (2019). AN INNOVATIVE WAY FOR EXTRACTION OF ESSENTIAL OIL COMPONENTS FROM PIMPINELLA ANISUM L. FRUITS. International Journal of Applied Pharmaceutics, 11(4), 83-87. https://doi.org/10.22159/ijap.2019v11i4.32825
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