EFFECT OF MOUTHRINSE CONTAINING IMMUNOGLOBULIN-Y ANTI-COMD STREPTOCOCCUS MUTANS + CHITOSAN ON QUANTITY OF SALIVARY STREPTOCOCCUS MUTANS IN CARIES AND CARIES-FREE SUBJECTS

  • ENDANG WINIATI BACHTIAR Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta - 10430, Indonesia.
  • ADANI NUR IMANINA Oral Science Research Center Faculty of Dentistry Universitas Indonesia, Jakarta, 10430, Indonesia.
  • RETNO D SOEJOEDONO Faculty of Veterinary Institute Pertanian Bogor, Indonesia, Bogor - 16680,
  • I WAYAN TEGUH WIBAWAN Faculty of Veterinary Institute Pertanian Bogor, Indonesia, Bogor - 16680, Indonesia.

Abstract

Objective: Immunoglobulin-Y (IgY) anti-ComD Streptococcus mutans is expected to be an alternative passive immunization agent against caries.
Chitosan which has an antibacterial property is expected to produce a better result. The aim of this study is to evaluate the effect of mouthrinse
containing IgY anti-ComD S. mutans + chitosan on the quantity of salivary S. mutans in caries and caries-free subjects.
Methods: Each subject group was given IgY anti-ComD S. mutans mouthrinse and IgY anti-ComD S. mutans + chitosan mouthrinse. Mouthrinse was
used twice a day for 6 days. Salivary S. mutans was cultured in TYS20B agar before and after treatment. The quantity of salivary S. mutans colonies
was counted manually.
Results: This study showed that mouthrinse containing IgY anti-ComD S. mutans + chitosan has the potential to decrease the quantity of salivary
S. mutans although not significantly.

Keywords: Caries, Chitosan, ComD protein, Immunoglobulin Y, Mouthrinse

References

1. Departemen Kesehatan. SKRT 2004. Jakarta: Badan Litbangkes; 2005.
2. Mount GJ, Hume WR, Ngo HC, Wolff M. Preservation and Restoration
of Tooth Structure. Queensland: Knowledge and Book Software; 2005.
3. Li YH, Tang N, Aspiras MB, Lau PC, Lee JH, Ellen RP, et al.
A quorum-sensing signaling system essential for genetic competence
in Streptococcus mutans is involved in biofilm formation. J Bacteriol
2002;184:2699-708.
4. Loo CY, Corliss DA, Ganeshkumar N. Streptococcus gordinii biofilm
formation: Identification of genes that code for biofilm phenotypes.
J Bacteriol 2000;182:1374-82.
5. Ferretti JJ, Shea C, Humphrey MW. Cross-reactivity of Streptococcus mutans
antigens and human heart tissue. Infect Immun 1980;30:69-73.
6. Smith DJ, Taubman MA. Vaccines Against Dental Caries Infection.
New Generation Vaccines; 1997. p. 914-30.
7. Kuroki M. Oral passive immunization using chicken egg yolk
immunoglobulins against bovine rotavirus and coronavirus infection.
Rec Res Dev Virol 1999;1:95-106.
8. Larsson A, Bålöw RM, Lindahl TL, Forsberg PO. Chicken antibodies:
Taking advantage of evolution – a review. Poult Sci 1993;72:1807-12.
9. Hatta H, Tsuda K, Ozeki M, Kim M, Yamamoto T, Otake S, et al.
Passive immunization against dental plaque formation in humans:
Effect of a mouth rinse containing egg yolk antibodies (IgY) specific to
Streptococcus mutans. Caries Res 1997;31:268-74.
10. Anggraeni. Prospek Gel Imunoglobulin-Y. Anti-Streptococcus mutans
Sebagai Bahan Imunisasi Pasif Anti Karies: Analisis Sifat Biologis
Gel Imunoglobulin-Y Anti Streptococcus mutans dan Pengaruhnya
Terhadap Proses Karies Pada Tikus Sprague Dawley [Disertasi].
Indonesia: Universitas Indonesia; 2010.
11. Chismirina S. Efek Imunoglobulin Y Sebagai Antiadhesin Pada
Pembentukan Biofilm Oleh S. mutans (Serotipe c,e,f) dan S. sobrins
(Serotipe d) Secara in vitro. Indonesia: Universitas Indonesia; 2006.
12. Winiati BE. Pembuatan Kemasan dan Uji Anti Karies IgY Kuning Telur
sebagai Suplemen Obat Kumur, Pasta Gigi dan Topikal Aplikasi; 2011.
13. Alysia H. Efek Pemberian Susu Kedelai yang Diperkaya dengan Kitosan
IgY Anti-Streptococcus mutans pada Tikus Malnutrisi: Evaluasi Pada
Pembentukan Biofilm Dental dan Berat Badan [Skripsi]. Universitas
Indonesia; 2009.
14. Razali N. Evaluasi Persistensi Immunoglobulin Y anti S. mutans pada
Saliva Tikus Malnutrisi Setelah Diberi Susu Kedelai Yang Diperkaya
Dengan IgY anti S. mutans dan kitosan [Skripsi]. Universitas Indonesia;
2009.
15. Febyani N. Efek Imunoglobulin Y (IgY) anti Streptococcus mutans dan
Kitosan Terhadap Autoaggregasi Mutans Streptococci Serotip c, d, e,
dan f [Skripsi]. Universitas Indonesia; 2009.
16. Dutta PK, Dutta J, Tripathi VS. Chitin and Chitosan: Chemistry,
Properties, and Application. Allahabad: Department of Chemistry,
Motilal Nehru National Institute of Technology; 2004.
17. Choi BK, Kim KY, Yoo YJ, Oh SJ, Choi JH, Kim CY, et al. In vitro
antimicrobial activity of a chitooligosaccharide mixture against
Actinobacillus actinomycetemcomitans and Streptococcus mutans. Int
J Antimicrob Agents 2001;18:553-7.
18. Costa EM, Silva S, Tavaria FK, Pintado MM. Study of the effects of
chitosan upon Streptococcus mutans adherence and biofilm formation.
Anaerobe 2013;20:27-31.
19. Tarsi R, Corbin B, Pruzzo C, Muzzarelli RA. Effect of low-molecularweight
chitosans on the adhesive properties of oral streptococci. Oral
Microbiol Immunol 1998;13:217-24.
20. Chia JS, Chang WC, Yang CS, Chen JY. Salivary and serum antibody
response to Streptococcus mutans antigens in humans. Oral Microbiol
Immunol 2000;15:131-8.
21. Nguyen SV, Icatlo FC Jr., Nakano T, Isogai E, Hirose K, Mizugai H,
et al. Anti-cell-associated glucosyltransferase immunoglobulin Y
suppression of salivary mutans streptococci in healthy young adults.
J Am Dent Assoc 2011;142:943-9.
22. Smith DJ, King WF, Godiska R. Passive transfer of immunoglobulin
Y antibody to Streptococcus mutans glucan binding protein B can
confer protection against experimental dental caries. Infect Immun
2001;69:3135-42.
23. Yonezu T, Sano Y, Kumazawa K, Fujita H, Kojima T, Shintani S, et al.
Effects of Topical Application of IgY on Salivary Streptococcus mutans.
Paper Presented at: 9th World Congress on Preventive Dentistry; 2009.
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How to Cite
BACHTIAR, E. W., IMANINA, A. N., SOEJOEDONO, R. D., & WIBAWAN, I. W. T. (2019). EFFECT OF MOUTHRINSE CONTAINING IMMUNOGLOBULIN-Y ANTI-COMD STREPTOCOCCUS MUTANS + CHITOSAN ON QUANTITY OF SALIVARY STREPTOCOCCUS MUTANS IN CARIES AND CARIES-FREE SUBJECTS. International Journal of Applied Pharmaceutics, 11(1), 120-122. https://doi.org/10.22159/ijap.2019.v11s1.AR174
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