THE TOXICITY OF MAULI BANANA (MUSA ACUMINATA) STEM WATER EXTRACT ON BONE MARROW MESENCHYMAL STEM CELL IN VITRO

  • AMY NINDIA Department of Oral Biology, Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, Indonesia
  • DIDIT ASPRIYANTO Department of Oral Biology, Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, Indonesia
  • MAHARANI LAILLYZA APRIASARI Department of Oral Biology, Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, Indonesia.
  • SELVIANA RIZKY Department of Oral Biology, Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, Indonesia

Abstract

Objective: Since mesenchymal stem cells (MSC) can differentiate into bone, cementum, and periodontal ligament, they can be used to treat aggressive
periodontitis. The limited number of MSCs requires replenishment of growth factor in the cell culture process. Since growth factor is quite expensive,
an alternative material is needed. Mauli banana stem has antioxidant and immunomodulatory properties. Methanol extract of Mauli banana stem is
known to be toxic toward MSCs; therefore, another solvent with a non-toxic effect is needed, such as a water solvent. We analyzed the toxicity of Mauli
banana stem water extract on MSC in vitro.
Methods: In this laboratory experimental (true experimental) study with a Post-test Only Control Group Design, MSC cultures were treated with
Mauli banana stem water extract at 10, 20, 40, 60, 80, and 100 mg/mL dosages. One group without any treatment served as a control group and one
was a media control group. Each group was incubated for 24 h and then was given 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
reagent and analyzed by an enzyme-linked immunosorbent assay (ELISA) reader.
Results: One-way analysis of variance showed a significant difference.
Conclusion: Mauli banana stem water extracts at 10, 20, 40, and 60 mg/mL were not toxic toward MSC in vitro, while dosages of 80 and 100 mg/mL
dosage were toxic.

Keywords: Mauli banana stem extract, Mesenchymal stem cell, Toxicity

References

1. Herawati D. Combination therapy for root debridement and antibiotics
against aggressive periodontitis. Majalah Kedokt Gigi Indones
2011;18 Suppl 2:200-4.
2. Newman MG, Takei HH, Carranza FA. Carranza’s Clinical
Periodontology. Maryland Heights, MO: Elsevier Health Science;
2012. p. 429.
3. Alpiste Illueca FM, Buitrago Vera P, de Grado Cabanilles P, Fuenmayor
Fernandez V, Gil Loscos FJ. Periodontal regeneration in clinical
practice. Med Oral Patol Oral Cir Bucal 2006;11:E382-92.
4. Jain A, Agarwal A, Gokhale ST, Manjunath S, Mishra N. Periodontal
ligament stem cell: An update. J Adv Clin Res 2014;1:20-2.
5. Kawaguchi H, Hirachi A, Hasegawa N, Iwata T, Hamaguchi H,
Shiba H, et al. Enhancement of periodontal tissue regeneration by
transplantation of bone marrow mesenchymal stem cells. J Periodontol
2004;75:1281-7.
6. Stolzing A, Jones E, McGonagle D, Scutt A. Age-related changes in
human bone marrow-derived mesenchymal stem cells: Consequences
for cell therapies. Mech Ageing Dev 2008;129:163-73.
7. Apriasari ML, Iskandar T, Suhartono E. Bioactive compounds and
antioxidant activity of methanol extract mauli bananas (Musa Sp) stem.
Int J Biosci Biochem Bioinform 2014;4 Suppl 2:110-5.
8. Noor WF, Aprianti N, Saputra SR, Apriasari ML, Suhartono E.
Oxidative stress on buccal mucosa wound in rat and rule of topical
application of ethanolic extract of maulibanana (Muca acuminata)
stem. J Trop Life Sci 2015;5 Suppl 2:84-7.
9. Son Y, Cheong YK, Kim NH, Chung HT, Kang DG, Pae HO, et al.
Mitogen-activated protein kinases and reactive oxygen species: How can
ROS activate MAPK pathways? J Signal Transduct 2011;2011:792639.
10. Artati, Kriswiyanti E, Fadilah. Effect of stirring rotational speed and
operating temperature on tannin extraction from cashew with acetone
solvent. Ekuilibrium 2007;6 Suppl 1:33-8.
11. Carabelly AN, Putra ST, Suardita K. The toxicity of methanol extract
of mauli banana stem (Musa acuminate) against bone marrow
mesenchymal stem cell in vitro. Dentino 2017;2 Suppl 1:24-8.
12. Hosein HK, Zinab D. Phenolic compounds and antioxidant activity of
henna leaves extracts (Lawsonia inermis L.). World J Daily Food Serv
2007;2 Suppl 1:38-41.
13. Freshney R. Culture Animal cell: A Manual of Basic Technique and
Specialized Application. 6th ed. New Jersey: John Wiley and Sons, Inc.;
2010. p. 187-94.
14. Romadanu, Rachmawati SH, Lestari SD. Test of activity antioxidant
Nelumbo nucifera extract. J Fish Tech 2014;3 Suppl 1:1-7.
15. Ashok PK, Upadhayaya K. Tannins are astringents. J Pharmacogn
Phytochem 2012;1 Suppl 3:45-9.
16. Ling LT, Palanisamy UD, Cheng HM. Prooxidant/antioxidant ratio
(proantidex) as a better index of net free radical scavenging potential.
Molecules 2010;15:7884-92.
17. Jordan LG. Tannic acid Crosslinked Collagen Type I for Prevention of
Local HER2-Positive Breast Cancer Recurrent; 2016. Available from:
https://www.tigerprints.clemson.edu/all_theses/2571/. [Last accessed
on 2018 Jan 16].
18. Jaganathan SK, Mandal M. Involvement of non-protein thiols,
mitochondrial dysfunction, reactive oxygen species and p53 in honeyinduced
apoptosis. Invest New Drugs 2010;28:624-33.
19. Kumar V, Abbas AK, Aster J. Basic Pathology. Philadelphia, PA:
Elsevier; 2017. p. 2-28.
20. Chi Z, Ma X, Cui G, Li M, Li F. Cinnamtannin B-1 regulates cell
proliferation of spinal cord astrocytes and protects the cell from
oxygen-glucose-serum deprivation/reoxygenation-induced apoptosis.
Int J Mol Sci 2013;14:15827-37.
21. Sun X, Gao RL, Lin XJ, Xu WH, Chen XH. Panax notoginseng
saponins induced up-regulation, phosphorylation and binding activity
of MEK, ERK, AKT, PI-3K protein kinases and GATA transcription
factors in hematopoietic cells. Chin J Integr Med 2013;19:112-8.
22. Yang J, Tan H, Zhai H, Wang Q, Zhao N, Cai Y, et al. Research on
chemical composition and ensiling characteristics of banana stems and
leaves. Adv Mater Res 2012;347:1647-51.
23. Ito Y, Pandey P, Place A, Sporn MB, Gribble GW, Honda T, et al.
The novel triterpenoid 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid
induces apoptosis of human myeloid leukemia cells by a caspase-8-
dependent mechanism. Cell Growth Differ 2000;11:261-7.
24. Taher M, Majid FA, Sarmidi MR. A proanthocyanidin from
Cinnamomum zeylanicum stimulates phosphorylation of insulin
reseptor in 3T3-L1 adipocytes. J Teknol 2006;44:53-68.
25. Haston S, Pozzi S, Carreno G, Manshaei S, Panousopoulos L, Gonzalez-
Meljem JM, et al. MAPK pathway control of stem cell proliferation
and differentiation in the embryonic pituitary provides insights into
the pathogenesis of papillary craniopharyngioma. Development
2017;144:2141-52.
26. Chen J, Crawford R, Chen C, Xiao Y. The key regulatory roles of the
PI3K/Akt signaling pathway in the functionalities of mesenchymal
stem cells and applications in tissue regeneration. Tissue Eng Part B
Rev 2013;19:516-28.
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NINDIA , A., ASPRIYANTO, D., APRIASARI, M. L., & RIZKY, S. (2019). THE TOXICITY OF MAULI BANANA (MUSA ACUMINATA) STEM WATER EXTRACT ON BONE MARROW MESENCHYMAL STEM CELL IN VITRO. International Journal of Applied Pharmaceutics, 11(1), 184-186. https://doi.org/10.22159/ijap.2019.v11s1.16762
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