DEVELOPMENT AND VALIDATION OF REVERSED PHASE HPLC-PDA METHOD FOR THE QUANTIFICATION OF CHRYSIN IN SOLID LIPID NANOPARTICLES
Objective: The main aim of the present study was to develop and validate a simple, precise and accurate Reversed-Phase HPLC-PDA method for the quantitative determination of Chrysin in solid lipid nanoparticles (SLNs).
Methods: The RP-HPLC-PDA system equipped with a C-18 reversed-phase column (250 × 4.6 mm, particle size 5 μm) was employed in the present study. HPLC grade methanol and water in 85:15 (v/v) ratio was selected as the mobile phase at flow rate of 1 ml/min under an ambient column oven temperature. The detection wavelength was kept at 268 nm. Validation of developed method was performed according to the ICH guidelines.
Results: The developed reversed-phase HPLC-PDA method was found to be linear in the concentration range of 0.2-10 µg/ml with a correlation coefficient of 0.999. The method was also observed to be precise with % relative standard deviation (RSD) below 2%. The limit of detection and limit of quantification of this method were found to be 0.05µg/ml and 0.14µg/ml, respectively. The percent recovery of the developed method was estimated to more than 99%.
Conclusion: The developed HPLC method can be utilized for the determination of Chrysin with a high degree of accuracy, precision, robustness, specificity in solid lipid nanoparticles in the presence of excipients.
2. Pichichero E, Cicconi R, Mattei M, Muzi MG, Canini A. Acacia honey and chrysin reduce proliferation of melanoma cells through alterations in cell cycle progression. Int J Oncol 2010;37:973-81.
3. Hadjmohammadi MR, Saman S, Nazari SJ. Separation optimization of quercetin, hesperetin and chrysin in honey by micellar liquid chromatography and experimental design. J Sep Sci 2010;33:3144-51.
4. Kasala ER, Bodduluru LN, Barua CC. Chrysin and its emerging antineoplastic effects. Cancer Gene Ther 2016;23:43.
5. Jung J. Emerging utilization of chrysin using nanoscale modification. J Nanomater 2016;1-7. http://dx.doi.org/10.1155/2016/2894089
6. Sun LP, Chen AL, Hung HC, Chien YH, Huang JS, Huang CY, et al. Chrysin: a histone deacetylase 8 inhibitor with anticancer activity and a suitable candidate for the standardization of chinese propolis. J Agric Food Chem 2012;60:11748–58.
7. Lirdprapamongkol K, Sakurai H, Abdelhamed S, Yokoyama S, Maruyama T, Athikomkulchai S, et al. A flavonoid chrysin suppresses hypoxic survival and metastatic growth of mouse breast cancer cells. Oncol Rep 2013;30:2357–64.
8. Walle T, Otake Y, Brubaker JA, Walle UK, Halushka PV. Disposition and metabolism of the flavonoid chrysin in normal volunteers. Br J Clin Pharmacol 2001;51:143–6.
9. Kadian R. Nanoparticles: a promising drug delivery approach. Asian J Pharm Clin Res 2018;11:30-5.
10. Dong D, Quan E, Yuan X, Xie Q, Li Z. Sodium oleate-based nanoemulsion enhances oral absorption of chrysin through inhibition of UGT-mediated metabolism. Mol Pharm 2016;14:2864-74.
11. Humberstone AJ, Charman WN. Lipid-based vehicles for the oral delivery of poorly water soluble drugs. Adv Drug Delivery Rev 1997;25:103–28.
12. Ansari MJ, Anwer MK, Jamil S, Al-Shdefat R, Ali BE, Ahmad MM, et al. Enhanced oral bioavailability of insulin-loaded solid lipid nanoparticles: pharmacokinetic bioavailability of insulin-loaded solid lipid nanoparticles in diabetic rats. Drug Delivery 2015;23:1972–9.
13. Chique KR, Puertas AM, Romero-Cano MS, Rojas C, Urbina Villalba G. Nanoemulsion stability: experimental evaluation of the flocculation rate from turbidity measurements. Adv Colloid Interface Sci 2012;178:1–20.
14. Qian C, Decker EA, Xiao H, McClements DJ. Physical and chemical stability of? beta-carotene-enriched nanoemulsions: influence of pH, ionic strength, temperature, and emulsifier type. Food Chem 2012;132:1221–9.
15. Qu J, Zhang L, Chen Z, Mao G, Gao Z, Lai X, et al. Nanostructured lipid carriers, solid lipid nanoparticles, and polymeric nanoparticles: which kind of drug delivery system is better for glioblastoma chemotherapy. Drug Delivery 2016;23:3408–16.
16. Singh RP, Ramarao P. Accumulated polymer degradation products as effector molecules in cytotoxicity of polymeric nanoparticles. Toxicol Sci 2013;136:131–43.
17. Müller RH, Radtke M, Wissing SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Adv Drug Delivery Rev 2002;54:S131-55.
18. Shahadali K, Garg A, Wahajuddin M. Development and evaluation of Chrysin-Phospholipid complex loaded solid lipid nanoparticles-storage stability and in vitro anticancer activity. J Microencapsulation 2018;35:600-17.
19. Kim KS, Shin JS, Park Y, Lee S, Kim YB, Kim B. High-performance liquid chromatographic analysis of chrysin derivatives on a nova-pak | C18 column. Arch Pharm Res 2002;25:613–6.
20. Pellati F, Orlandinia G, Pinetti D, Benvenutia S. HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts. J Pharma Biomed Anal 2011;55:934–48.
21. ICH Q2(R1) Validation of analytical procedures, text and methodology. International conference on Harmonization; 2016.
22. Balaji N, Sivaraman VR, Neeraja P. A validated UPLC method for the determination of process-related impurities in the antimigraine bulk drug. J Appl Chem 2013;3:20-8.
23. Joana G, Zelia B. Validation of two spectrophotometric methods for fluoxetine quantification. Int J Pharm Pharm Sci 2016;8:72-8.
24. Sesharao M, Madhavarao V. A new validated simultaneous reversed-phase high-performance liquid chromatography assay method for estimation of two flavones (baicalein and chrysin) in API drugs. Asian J Pharm Clin Res 2018;11:351-6.
25. Krishnaphanisri P, Sundararajan R. Development and validation of new RP-UPLC method for the determination of cefdinir in bulk and dosage form. Int J Pharm Pharm Sci 2018;10:178-84.
This work is licensed under a Creative Commons Attribution 4.0 International License.