• Ediati Ediati Universitas Gadjah Mada
  • Teuku Nanda Saifullah Department of Pharmaeutics, Faculty of Pharmacy, Uniersitas Gadjah Mada
  • Rumiyati Rumiyati Department of Pharmaeutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada
  • DUNSTANIA Maria Faculty of Pharmacy, Universitas Gadjah Mada
  • MARIA QUEEN Vienka Faculty of Pharmacy, Universitas Gadjah Mada
  • ERNA MARIANA Sinaga Faculty of Pharmacy, Universitas Gadjah Mada


Objective: The study aimed to formulate standardized polysaccharide fraction of Noni (SPFN) fruit into syrup preparations to fulfill the acceptability requirement of National Agency of Drugs and Food Control [Badan Pengawas Obat dan Makanan Republik of Indonesia (BPOM RI)] so that it can be used in the community as immunostimulant.

Methods: The optimization of the formula A and B were done using Simplex Lattice Design (SLD) method and its stability tested under extreme temperature change effect using thaw cycling method by evaluating the physical properties, microbial contamination and immunostimulant activity. MTT method was used to determine immunostimulant activity of syrup against lymphocyte proliferation in vitro.

Results: Based on the results of stability tests using pH, viscosity, taste, amount of bacteria, yeast and mold parameters and fulfilled the acceptability requirement of BPOM RI. Formula A could maintain better stability than formula B. Formula A syrup used for immunostimulant activity testing that could increase lymphocyte proliferation at concentration of 13.33 to 106.64 μg/μl and it’s stable under the influence of extreme temperature changes.

Conclusion: Formula A could be used to produce SPFN syrup which has a stable physical properties and immunostimulant activity beside no microbial contamination that fulfill requirements of BPOM RI.

Keywords: Noni fruit, Polysaccharide, Syrup, Thaw cycling, MTT, Immunostimulant


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How to Cite
Ediati, E., Saifullah, T. N., Rumiyati, R., Maria, D., Vienka, M. Q., & Sinaga, E. M. (2019). FORMULATION AND IMMUNOSTIMULANT STABILITY POLYSACCHARIDE SYRUP OF FRACTION NONI (Morinda citrifolia L.) FRUITS. International Journal of Applied Pharmaceutics, 11(5). Retrieved from
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