FTIR SPECTROSCOPIC TRENDS AND DNA DAMAGE IN RABBIT LENS DUE TO LONG RUN OF TAMOXIFEN TREATMENT

  • MERVAT A. A. Biophysics and Laser Science Unit, Visual Science Department, Research Institute of Ophthalmology-Giza, Egypt
  • EMAN S. M. Biophysics and Laser Science Unit, Visual Science Department, Research Institute of Ophthalmology-Giza, Egypt
  • EMAN M. A. Biophysics and Laser Science Unit, Visual Science Department, Research Institute of Ophthalmology-Giza, Egypt

Abstract

Objective: The aim of the present work is to evaluate the molecular structure changes of the lens of rabbits and DNA damage of epithelial cells due to tamoxifen administration.


Methods: Twenty four healthy New Zealand white rabbits were divided into 2 main groups. The first group is served as control (n=12) kept untreated, second one is Tamoxifen administrative group (n=12) received orally daily dose of 15 mg/kg. Rabbits were decapitated after 2, 4, 6 and 8 mo, respectively. Using fourior transform infrared (FTIR) to study the molecular structure changes due to tamoxifen and comet assay analysis for discovering DNA damage.


Results: FTIR data indicated that tamoxifen affects structural components in NHOH and fingerprint region. Increases of β-turns of the protein secondary structure while, reducing the content of both α-helix after 8 mo and Turns appeared for all periods of administrative tamoxifen were observed. On the other hand tamoxifen induced a statistically significant increase in comet assay parameters as tail moment compared to control animals that indicated DNA damage due to single or double strand break.


Conclusion: Tamoxifen uses for more than 6 mo may lead to changes in the molecular structure of the lens and damage of DNA cells. An ophthalmic baseline examination prior to anti-cancer treatment may help detect any pre-existing ocular condition and lead to reduction of ocular side effects when predisposed patients are screened and examined regularly during and after chemotherapeutic therapy.

Keywords: Chemotherapy, Tamoxifen, Lens, Cataract, FTIR, Comet assay

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A. A., M., S. M., E., & M. A., E. (2019). FTIR SPECTROSCOPIC TRENDS AND DNA DAMAGE IN RABBIT LENS DUE TO LONG RUN OF TAMOXIFEN TREATMENT. International Journal of Applied Pharmaceutics, 11(5), 90-95. https://doi.org/10.22159/ijap.2019v11i5.34109
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