CHARACTERIZATION AND ANTI-NEOPLASTIC POTENTIAL OF PHYTOFABRICATED SILVER NANOPARTICLES ON HUMAN BREAST CANCER CELL LINE (MCF-7)

  • CHINNAIAH KUPPUSWAMY AMUDHA School of Research and Innovation, CMR University, HRBR Layout, Bangalore 560043, India
  • FARAH DEEBA School of Research and Innovation, CMR University, HRBR Layout, Bangalore 560043, India
  • PETHANNAN RAJARAJAN Department of Microbiology, Center for Research and PG Studies, Indian Academy Degree College-Autonomous, Hennur Cross, Kalyan Nagar, Bangalore 560043, India

Abstract

Objective: The surging cancer incidence has necessitated the synthesis, characterization and production of green synthesized silver nanoparticles and its effective use. The present study was carried out to assess the antineoplastic potential of the green synthesized silver nanoparticles against breast cancer cell lines.


Methods: The rapid biological synthesis of silver nanoparticles was done using Withania somnifera, Aegle marmelos and Taraxacum officinale. The synthesized nanoparticles were characterized using Scanning Electron Microscope (SEM), Energy Dispersive X-Ray Spectroscopy (EDX) and Fourier Transform Infra-Red (FTIR) analysis. Further, the antineoplastic potential of the green synthesized nanoparticles were assessed by MTT assay at an ascending concentration of 25, 50, 100, 250, 500μg/ml against MCF-7 (Breast) cancer cell lines.


Results: The synthesized nanoparticles were characterized using SEM, EDX and FTIR analysis which confirmed the formation of silver nanoparticles and were of irregular shape with a size of 26 nm, 18 nm, 94 nm, and its IC50 value was 251.2563±0.59µg/ml, 215.5172±0.41µg/ml and 205.7613±0.37µg/ml for Aegle marmelos, Taraxacum officinale and Withania somnifera respectively against MCF-7 cells.


Conclusion: The investigation unveils the fact that phytofabricated AgNPs could be exploited as an effective antineoplastic agent for further medical and pharmaceutical applications.

Keywords: Green synthesis, Silver nanoparticles, Breast cancer

Author Biography

PETHANNAN RAJARAJAN, Department of Microbiology, Center for Research and PG Studies, Indian Academy Degree College-Autonomous, Hennur Cross, Kalyan Nagar, Bangalore 560043, India

Professor & Head, Department of Microbiology

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AMUDHA, C. K., DEEBA, F., & RAJARAJAN, P. (2019). CHARACTERIZATION AND ANTI-NEOPLASTIC POTENTIAL OF PHYTOFABRICATED SILVER NANOPARTICLES ON HUMAN BREAST CANCER CELL LINE (MCF-7). International Journal of Applied Pharmaceutics, 11(6), 70-77. https://doi.org/10.22159/ijap.2019v11i6.34528
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