LIGHT SCATTERING IN RESEARCH AND QUALITY CONTROL OF DEUTERIUM DEPLETED WATER FOR PHARMACEUTICAL APPLICATION

  • Alena Koldina Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation

Abstract

Objective: Development of a methodology for measuring the deuterium content in water for pharmaceutical purposes by laser light scattering on the basis of ideas about the cluster structure of water.


 


Methods: Samples of industrially manufactured drinking water from different manufacturers with varying deuterium content from 10 ppm to 115 ppm. For the titration of laboratory samples of deuterium depleted water in increments of 5 ppm the following reagents were used: Water, deuterium-depleted (≤1 ppm (D2O, ALDRICH); Deuterium oxide/ Heavy water/Water-d2 (99.9 atom % D, ALDRICH; water Mili-Q (specific resistance 18.2 MΩ·cm at 25оС, ТОС ≤ 5 ppb, Merck Millipore). The determination of deuterium content in samples of industrially manufactured water and water obtained in a laboratorial manner was carried out by the method of low-angle laser light scattering (LALLS) at the Mastersizer (Malvern Instruments) analyser and using a working measuring tool – laser dispersion meter/ MDL («Cluster-1», Russia/Ukraine). The statistical methods – packages OriginPro®9.


 


Results: It was found that the content of isotopologues in water leads to changes in the morphology of giant heterogeneous water clusters (GHC). The results of low-angle laser light scattering in the water samples under investigation showed the dependence of the GHC "dispersability" expressed in the differentiation of the laser obscuration values (I ‒I0), the volume concentration, w,% and the curves of the volume size distribution function of the variations of the isotopic composition of water. The results of the LALLS-method correlate with the calculations made by us using a computer model and reliably describe the analytical characteristics of the samples for the comparative research using physical and chemical descriptors.


Conclusion: When identifying deuterium depleted water, it should be taken into account not only the indicators that determine its pharmacopoeial quality, but also the D/H ratio, because even small changes in the natural isotopic composition of water lead to significant biological effects. Our proposed approach using physical and mathematical model makes possible the exact calculation of individual signs of deuterium depleted water as the pharmaceutical object of study.

Keywords: Deuterium depleted water, isotope control, D/H ratio in water, descriptors, LALLS method

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Koldina, A. (2019). LIGHT SCATTERING IN RESEARCH AND QUALITY CONTROL OF DEUTERIUM DEPLETED WATER FOR PHARMACEUTICAL APPLICATION. International Journal of Applied Pharmaceutics, 11(5). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/34672
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