ISOLATION OF ACTIVE COMPOUND FROM RED POMEGRANATE (PUNICA GRANATUM L.) SEEDS ETHANOLIC EXTRACTS
Objective: Pomegranate (Punica granatum L.) is rich in antioxidants and antibacterials, lending itself to beneficial effects on health improvement. The seeds contain a variety of active compounds. The purpose of this research was to isolate the active compound of red pomegranate seeds through ethanolic extraction.
Methods: Pomegranate seeds were extracted into 24 hexane fractions of the concentrated crude extract and left in open vials until all the solvent evaporated, leaving behind crystals. Crystals in vials 11, 12, and 13 were purified using n-hexane, ethyl acetate, methanol, chloroform, acetone and isopropyl alcohol as the solvents. The crystals were monitored with Thin Layer Chromatography (TLC) on a Kiesel gel 254 GF plate (e. Merck) using the developing system of hexane: ethyl acetate: formic acid (1:1:0.1) mixture and a UV light spot viewer with 366 wavelength emissions. Purification test with TLC two directions using hexane: ethyl acetate: formic acid (1:1:0.1) mixture as the eluents for the first development, continued with the second development using chloroform: methanol: acetate acid (9:0.5:0.5) mixture. The Retention Factor (Rf) value for the spot was measured.
Results: Chromatogram showed only 1 spot with Rf value 0.88.
Conclusion: There is one pure (active) isolate in red pomegranate seeds ethanolic extract.
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