EFFECT OF LIPOPOLYSACCHARIDE INDUCTION OF PORPHYROMONAS GINGIVALIS ON OSTEOCLAST AND OSTEOBLAST CELL NUMBER OF WISTAR RATS’ (RATTUS NORVEGICUS) ALVEOLAR BONE

  • TANTIN ERMAWATI Department of Biomedical Science, Faculty of Dentistry, University of Jember, Jember, Indonesia
  • DESY FUTRI INTAN GANDINI ABDI NAGARI Faculty of Dentistry, University of Jember, Jember, Indonesia
  • DEPI PRAHARANI Department of Periodontology, Faculty of Dentistry, University of Jember, Jember, Indonesia
  • DESI SANDRA SARI Department of Periodontology, Faculty of Dentistry, University of Jember, Jember, Indonesia

Abstract

Objective: This experimental laboratory research too serves the effect of LPS P. gingivalis induction on the osteoclast and osteoblast cell number of Wistar rats’ alveolar bone.


Methods: in vivo laboratory, experimental research was conducted using posttest only control group design. The samples were 20 male Wistar rats divided into 4 groups. Groups I and II were groups injected using LPS P. gingivalis for 6 w and were decapited on the day 3 and day 7, groups III and IV were the control groups (not injected using LPS P. gingivalis) and decapited on the day 3 and day 7. Subsequently, conducting tissue preparation, staining using haematoxilin eosin, and calculating the number of osteoclasts and osteoblasts cells using a microscope (Optilab) with 400x magnification. The results of osteoblast and osteoclast cell calculation were analyzed using ANOVA and LSD one-way test.


Results: Induction of LPS P. gingivalis affected the number of osteoclasts and osteoblasts cell number of Wistar rats’ alveolar bone.


Conclusion: Induction of LPS P. gingivalis in Wistar rats (Rattusnorvegicus) increases osteoclast cell number and decreases osteoblast cell number.

Keywords: Lipopolysaccharide, Osteoblast, Osteoclasts, Periodontitis, Porphyromonas gingivalis

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TANTIN ERMAWATI, DESY FUTRI INTAN GANDINI ABDI NAGARI, PRAHARANI, D., & SARI, D. S. (2019). EFFECT OF LIPOPOLYSACCHARIDE INDUCTION OF PORPHYROMONAS GINGIVALIS ON OSTEOCLAST AND OSTEOBLAST CELL NUMBER OF WISTAR RATS’ (RATTUS NORVEGICUS) ALVEOLAR BONE. International Journal of Applied Pharmaceutics, 11(4), 64-67. https://doi.org/10.22159/ijap.2019.v11s4.35294
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