DEXTRAN SULFATE STABILIZED SILVER NANOPARTICLE: NEXT GENERATION EFFICIENT THERAPY FOR CANCER

  • SHARMILA CHANDRAN Department of Physics, PSGR Krishnammal College for Women, Coimbatore 641004, Tamil Nadu. India
  • THILAGAVATHY PONNUSAMY Department of Physics, PSGR Krishnammal College for Women, Coimbatore 641004, Tamil Nadu. India
  • DINESH BHEEMAN Biology Division, Department of Applied Sciences, Higher College of Technology, Muscat-133, Sultanate of Oman
  • RANJITH KUMAR RAJAMANI Department of Biotechnology, Kongunadu Arts and Science College, G. N Mills, Coimbatore 641029, Tamil Nadu, India
  • CHANDAR SHEKAR BELLAN Department of Physics, Kongunadu Arts and Science College, G. N Mills, Coimbatore 641029, Tamil Nadu, India

Abstract

Objective: Synthesize silver nanoparticles using a green synthesis approach and encapsulate silver nanoparticles with a naturally occurring polymer, particularly of an-hydroglucose type, like dextran sulfate sodium salt and to study its anticancer activity.


Methods: Green synthesis approach is been employed in the synthesis of silver nanoparticles using Psidium guajava leaf extract. The nanoparticles were then encapsulated with dextran sulfate biopolymer and the nanoparticles were subjected to different characterization techniques. The structure of the synthesized nanoparticles was analyzed using X-ray diffraction analysis, the presence of different functional groups was analyzed by FTIR studies. Size and morphology of the prepared nanoparticles were investigated using FESEM analysis. Anticancer activity of the synthesized nanoparticles was tested against the MCF-cell line.


Results: The XRD analysis shows the crystalline nature of the synthesized nanoparticles. The stretching and vibrating modes of different functional groups were confirmed by FTIR result. The SEM image confirmed the presence of spherical shaped nanoparticles and the TEM image confirmed the average size of the particles to be around 24 nm. The Ag-DS NPs showed 91% cell inhibition for the concentration of 100 μg/ml, indicating the cytotoxicity of the nanoparticles against MCF-7 cell line.


Conclusion: Dextran sulfate stabilized silver nanoparticles show potent anticancer activity against MCF-7 cell line.

Keywords: Silver nanoparticles, Biopolymer, Dextran sulfate, Anticancer activity

Author Biographies

RANJITH KUMAR RAJAMANI, Department of Biotechnology, Kongunadu Arts and Science College, G. N Mills, Coimbatore 641029, Tamil Nadu, India

Assistant Professor, Department of Biotechnology

CHANDAR SHEKAR BELLAN, Department of Physics, Kongunadu Arts and Science College, G. N Mills, Coimbatore 641029, Tamil Nadu, India

Assistant Professor, Departmetn of Physics

References

1. Crooks RM, Zhao M, Sun L, Chechik V, Yeung LK. Dendrimer-encapsulated metal nanoparticles: Synthesis, characterization and applications to catalysis. Acc Chem Res 2001;34:181-90.
2. Lee KS, El-Sayed MA. Gold and silver nanoparticles in sensing and imaging: sensitivity of plasmon response to size, shape and metal composition. J Phys Chem B 2006;110:19220-5.
3. Prashant KJ, Huang X, El-Sayed IH, El-Sayed MA. Nobel metal on the nanoscale: Optical and Photothermal properties and some applications in imaging, sensing, biology and medicine. Acc Chem Res 2008;41:1578-86.
4. Carlson C, Hussain SM, Schrand AM, Braydich Stolle LK, Hess KL, Jones RL, et al. Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B 2008;112:13608-19.
5. Divya J, Sneh P, Jainey PJ. Antimicrobial potential of hydrogel incorporated with PLGA nanoparticles of Crossandra Infundibuliformis. Int J Appl Pharm 2019;11:1-5.
6. Maria B. An introduction to nanoparticles and nanotechnology. Morgan Claypool Pub; 2015. p. 1-14.
7. Rankin JC, Jeanes A. Evaluation of the periodate oxidation method for structural analysis of dextrans. J Am Chem Soc 1954;76:4435-41.
8. Glisic S, Cakic M, Nikolic G, Danilovic B. Synthesis, characterization and antimicrobial activity of carboxymethyl dextran stabilized silver nanoparticles. J Mol Struct 2015; 1084:345-51.
9. Kirthika P, Dheeba B, Sivakumar R, Sheik Abdulla S. Plant mediated synthesis of silver nanoparticles. Int J Pharm Pharm Sci 2014;8:304-10.
10. Sharmila C, Vinuppriya R, Chandran S, Chemmanda J, Chandarshekar B. Biosynthesis of PVA encapsulated silver nanoparticles. J Appl Res Tech 2016;14:319-24.
11. Mubarak Ali D, Thajuddin N, Jeganathan K, Gunasekaran M. Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Coll Surf B Biointer 2011;85:360-5.
12. Sunitha P, Rajeswari S, Rajiv P, Rajendran V, Seenivasan R. Green synthesis of silver nanoparticles from leaf extract of Aegle marmelos and evaluation of its antibacterial activity. Int J Pharm Pharm Sci 2015;6:169-73.
13. Dipanwita M, Mrinal KB, Biplab B, Joy S, Saswati S, Krishnendu A, et al. In situ synthesis, characterization, and antimicrobial activity of silver nanoparticles using water-soluble polymer. Inc J Appl Polym Sci 2011;122:2189–96.
14. Nguyen TK, Luke AWG. Fictionalization of nanoparticles for biomedical applications. Nano Today 2010;5:213–30.
15. Shipway A, Willner I. Nanoparticles as structural and functional units in surface-confined architectures. Chem Commun 2001;20:2035-45.
16. Antony Lawrence A, Thomas JPJ. Biogenic synthesis of silver nanoparticles using Manilkara hexandra (ROXB.) dubard stem bark extract and its physical, chemical characterization and pharmaceutical evaluation. Int J Appl Pharm 2019;11:79-88.
17. Rajeswari A, Vinoth J. Biogenic potential of stabilized silver nanoparticles using G. Sylvestrae and their biological assays. Int J Appl Pharm 2019;11:130-7.
18. Noorsaiyyidah DS, Nurul ACL, Mohd RJ, Roslina A. FTIR studies on silver–poly (methylmethacrylate) nanocomposites via In-situ polymerization technique. Int J Electrochem Sci 2012;7:5596-603.
19. Awwad AM, Salem NM, Abdeen AO. Biosynthesis of silver nanoparticles using Olea europaea leaves extract and its antibacterial activity. Nanosci Nanotech 2012;2:164-70.
Statistics
98 Views | 19 Downloads
Citatons
How to Cite
CHANDRAN, S., PONNUSAMY, T., BHEEMAN, D., RAJAMANI, R. K., & BELLAN, C. S. (2019). DEXTRAN SULFATE STABILIZED SILVER NANOPARTICLE: NEXT GENERATION EFFICIENT THERAPY FOR CANCER. International Journal of Applied Pharmaceutics, 12(1), 59-63. https://doi.org/10.22159/ijap.2020v12i1.35327
Section
Original Article(s)