• REVI G. H. NOVIKA Doctoral Program, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
  • BUDI SANTOSO Department of Obstetric and Gynecology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
  • WIDJIATI WIDJIATI Department of Embriology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.


Objective: The aim of the study is to investigate the influence of cortisol and Heat Shock Protein 70 (HSP70) on the stressed mice to maturation
promoting factor (MPF) expression intrafollicular.
Methods: Experimental laboratory with Randomized Post Test Only Control Group Design was carried out on intrafollicular mice. Divided into two
groups, experimental and control groups. The experimental group was given a 95 dB 4 hours/day noisy exposure for 5 days which was analogous to
psychological stress in humans and the control group was not given noisy exposure. Furthermore, both groups were examined for cortisol levels to
ensure stress in mice. Heat shock protein 70 (HSP70) expression was examined as the main regulatory protein for stress response and Maturation
promoting factor (MPF) expression which is a mediator for oocyte maturation.
Results: Psychological stress by 95 dB/4 hours/day noisy exposure for 5 days significantly increased serum cortisol levels in experimental group
(p=0.000) and HSP70 expression (p=0.000). The effect of Increased cortisol levels and HSP70 expression significantly decrease in MPF expression
Conclusion: The study concluded that psychological stress could be seen by increasing cortisol and HSP70 expression affected to decreasing MPF
expression intrafollicular

Keywords: Stress, Cortisol, Heat Shock Protein 70, Maturation promoting factor


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How to Cite
NOVIKA, R. G. H., SANTOSO, B., & WIDJIATI, W. (2019). THE EFFECT OF PSYCHOLOGICAL STRESS ON MPF INTRAFOLLICULAR. International Journal of Applied Pharmaceutics, 11(5), 127-130.
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