NANOPARTICLE BASED BIOSENSOR FOR PENICILLIN QUANTIFICATION IN PHARMACEUTICALS
Objective: The objective of this study was to develop a new biosensor system based on nanoparticle to determine penicillin in pharmaceuticals.
Methods: The characterization and optimization of the potentiometric penicillin biosensor (PB) were prepared by using synthesized surface-dependent and surface-independent ZnO nanoparticles named ZnO nanorods and chitosan were carried out. It was preferred ZnO nanorod because of its electrical, optical, physical and photocatalyst properties, biocompatibility and non-toxicity in the construction of the penicillin biosensor.
Results: The operating range was obtained as 10-1-10-3M, the optimum buffer concentration was 10 mmol, optimum pH was 7.4 and the optimum temperature was 25 °C for the PB. The PB has advantages in terms of short response time, long enough shelf life, cheap, and easy elaborate.
Conclusion: Whether the biosensor can be used to determine penicillin and accurately measure penicillin, the amount of penicillin in a commercial pharmaceutical preparation named Alfoxil was successfully made by using our prepared penicillin biosensor.
2. Wu Y, Tang L, Huang L, Han Z, Wang J, Pan H. A low detection limit penicillin biosensor based on single garphene nanosheets preadsorbed with hematein/ionic liquids/penicillinase. Mat Sci Eng C 2014;39:92-9.
3. Mondol R, Paul S, Ray S, Maiti S. Polymer?c nanocarr?ers: a promising research avenue for the delivery of antihiv drugs. Int J Appl Pharm 2010;2:1-5.
4. Karliana D, Anwar E, Bahtiar A. Formulation and evaluation of quercetin nanoparticle gel for osteoarthritis. Int J Appl Pharm 2019;11:54-9.
5. Seki A, Ikeda S, Kubo I, Karube I. Biosensors based on light-addressable potentiometric sensors for urea, penicillin and glucose. Anal Chim Acta 1998;373:9-13.
6. Ozturk SS, Palsson BO. Chemical decomposition of glutamine in cell culture media: effect of media type, pH, and serum concentration. Biotechnol Progr 1990;6:121–8.
7. Guilbault GG, Kauffman JM. Enzyme-based electrodes as analytical tools. Biotechnol Appl Biochem 1987;9:95-113.
8. Updike SJ, Hicks GP. The enzyme electrode. Nature 1967;214:986-8.
9. Fabre B, Simonet J. Electractive polymers containing crown ether or polyether ligands as cation-responsive materials. Coordination Chem Rev 1998;178-180:1211-50.
10. Clark LC, Lyons C. Electrode system for continuous monitoring in cardiovascular surgery. Ann NY Acad Sci 1962;102:29-45.
11. Li H, Xu B, Wang D, Zhou Y, Zhang H, Xia W, et al. Immunosensor for trace penicillin g detection in milk based on supported bilayer lipid membrane modified with the gold nanoparticle. J Biotechnol 2015;203:97-103.
12. Liu J, Liang L, Li G, Han R, Chen K. H+ISFET-based biosensor for determination of penicillin G. Biosens Bioelectron 1998;13:1023-8.
13. Thust M, Schoning MJ, Muller Veggian M, Kordos P, Luth H. A long-term stable penicillin-sensitive potentiometric biosensor with enzyme ?mmobilized by heterobifunctional cross-linking. Anal Chim Acta 1996;323:115-21.
14. Gonçalves LM, Callera WFA, Sotomayor MDPT, Bueno PR. Penicillinase-based amperometric biosensor for penicillin G. Electochem Commun 2014;38:131-3.
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