NANOPARTICLE BASED BIOSENSOR FOR PENICILLIN QUANTIFICATION IN PHARMACEUTICALS

EMINE  KARAKUS; CISEM  TURAN

doi:10.22159/ijap.2020v12i1.35906

EMINE KARAKUS Department of Chemistry, Faculty of Arts and Sciences, Yıldız Technical University: Esenler, 34290, İSTANBUL, Turkey
CISEM TURAN Department of Chemistry, Faculty of Arts and Sciences, Yıldız Technical University: Esenler, 34290, İSTANBUL, Turkey

DOI https://doi.org/10.22159/ijap.2020v12i1.35906

Abstract

Objective: The objective of this study was to develop a new biosensor system based on nanoparticle to determine penicillin in pharmaceuticals.

Methods: The characterization and optimization of the potentiometric penicillin biosensor (PB) were prepared by using synthesized surface-dependent and surface-independent ZnO nanoparticles named ZnO nanorods and chitosan were carried out. It was preferred ZnO nanorod because of its electrical, optical, physical and photocatalyst properties, biocompatibility and non-toxicity in the construction of the penicillin biosensor.

Results: The operating range was obtained as 10^-1-10^-3M, the optimum buffer concentration was 10 mmol, optimum pH was 7.4 and the optimum temperature was 25 °C for the PB. The PB has advantages in terms of short response time, long enough shelf life, cheap, and easy elaborate.

Conclusion: Whether the biosensor can be used to determine penicillin and accurately measure penicillin, the amount of penicillin in a commercial pharmaceutical preparation named Alfoxil was successfully made by using our prepared penicillin biosensor.

Keywords: Penicillin, Zinc oxide nanorod, Biosensor, Potentiometric, Chitosan

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