EVALUATION OF ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF HOLOPTELEA INTEGRIFOLIA (ROXB) PLANCH


Ravindra C Sutar, V K Kalaichelvan

Abstract


Holoptelea integrifolia (Roxb.) Planch has been used from long time in traditional medicine. The main objective of the work was to evaluate the antioxidant activity of leaves of Holoptelea integrifolia (Roxb.) Planch. The antioxidant activity of petroleum ether and methanolic extract of Holoptelea integrifolia (HI) leaves was evaluated using Hydroxyl Radical Scavenging Activity and Total Reduction Capability models. Preliminary Phytochemical investigation of the petroleum ether extract (PEHI) of Holoptelea integrifolia leaves reveals the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins, and carbohydrate while methanolic extract (MHI) of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins and carbohydrates. In case of Hydroxyl Radical Scavenging Activity both the HI extracts showed dose dependant increase in percent inhibition i. e. hydroxyl radical scavenging activity and thereby showed antioxidant activity however PEHI is more potent than MHI in this regard, while in case of Total Reduction Capability both the HI extracts have shown dose dependent increase in absorbance thereby dose dependant total reduction capacity indicating antioxidant activity. Further trend suggest that PEHI has more potent antioxidant potential than that of MHI. The results indicate that petroleum ether and methanol extracts contained such phytochemial compounds which are active in case of antioxidant activity using Hydroxyl Radical Scavenging Activity and Total Reduction Capability. Which support the ethnomedicinal application of the plant as an antioxidant agent.

Keywords: Holoptelea integrifoila leaves, Antioxidant, Hydroxyl Radical Scavenging Activity, Total Reduction Capability. 


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References


Yu BP. Cellular defences against damage from reactive oxygen species. Physiol Rev 1994;74:139.

Halliwel B, Gutteridge JMC. Free radicals in biology and medicine, 2nd edition, Clarendon Press: Oxford; 1988. p. 1.

Lata H, Ahuja GK. Role of free radicals in health and disease. Ind J Physio Allied Sci 2003;57:124.

Cotran RS, Kumar V, Collins T. in Robbin’s pathological basis of diseases, 6’th edition. Thomson Press I Ltd, Noida, India; 1999. p. 1.

Y u B P, Suescun E A, Yang SY. Effect of age related lipid peroxidation on membrane fluidity and phospholipase A2: modulation by dietary restriction. Mech Ageing Dev 1992;65:17.

Campbell I C, Abdulla E M. Strategic approaches to in vitro neurotoxicity, in Approaches and Methods: Neurotoxicity: Academic Press London; 1995. p. 495.

Marx J L. Oxygen free radicals linked to many diseases. Sci 1987;235:529.

Pratt D E. Natural antioxidant from plant material, in Phenolic compounds in food and their effects on health II: Antioxidants and Cancer prevention (ACS Symposium Series 507) edited by M Hang, C Ho and C Lee (American Chemical Society, Washigton D C); 1992. p. 54.

Kirtikar KR, Basu BD. Indian Medicinal Plants. 3rd edition. Sri Satguru Publications, New Delhi, India; 2000. p. 2292-4.

Prajapati ND, Purohit SS, Sharma AK. A Handbook of medicinal plants a complete source book. Agrobias. Jodhpur. India; 2003. p. 273.

Warrier PK, Nambiar VPK, Ramakutty C. Indian Medicinal Plants a compendium of 500 species. Orient longman private Limited 1995;3:162.

Nadkarni KM. Indian Materia Medica. 3rd Edition. Mumbai: Popular Prakashan Pvt. Ltd; 1982 . p. 651-52.

Khandelwal KR. Practical Pharmacognosy. Techniques and Experiments. 10th ed. Nirali Prakashan, Pune, India; 2006. p. 149-56.

Kunchandy E, Rao MNA. Oxygen radical scavenging activity of curcuminoid. Int J Pharmaco 1990;58:237.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thibarbituric acid reaction. Anal Biochem 1979;95:351.

Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:629-32.

Gulcin I, Oktay M, Kirecci E, Kufrevioglu OI. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L) seed extracts. Food Chem 2003;83:371–82.

Satyavati GV, Gupta AK, Neeraj T. Medicinal plants of India (ICMR, New Delhi) 1987;2:490.

Sharma PC, Yelne MB, Dennis TJ. Database on medicinal plants used in Ayurveda, Vol. 3, Delhi, Documentation and Publication Division, Central Council for Research in Ayurveda and Siddha; 2001. p. 404.

Liu F, Ng TB. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sci 2000;66:725-35.

Diplock AT. Will the ‘good fairies’ please prove to us that vitamin E lessens human degenerative disease? Free Rad Res 1997;27:511-32.

Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V. Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf Ex DC), Sage (Salvia triloba L.), and Black Tea (Camellia sinensis) extracts. J Agri Food Chem 2000;48:5030-4.




About this article

Title

EVALUATION OF ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF HOLOPTELEA INTEGRIFOLIA (ROXB) PLANCH

Date

31-10-2014

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Journal

International Journal of Applied Pharmaceutics
Vol 6, Issue 3 (Oct-Dec 2014) Page: 6-8

Online ISSN

0975-7058

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Authors & Affiliations

Ravindra C Sutar

V K Kalaichelvan


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