HAIR TONIC FORMULATION OF CLOVE LEAVES (SYZYGIUM AROMATICUM) ETHANOL EXTRACT AND THE EFFECTIVENESS ON RABBIT HAIR GROWTH
Objective: The purpose of this study was to determine the physical stability and effectiveness of hair growth in hair tonic preparations containing ethanol extract of clove leaves (Syzygium aromaticum) with a concentration variation of 5, 10, and 15%.
Methods: Formulations were evaluated, including organoleptic observation, pH checking, and preparation stability tests using the Climatic Chamber. The effectiveness of clove leaf extract as a hair tonic was tested by looking at hair growth in test animals.
Results: The results showed that the preparation was stable at high temperatures (40±2 °C) where the odor, color, clarity, and pH remained constant. Effectiveness test on rabbit hair growth showed significantly different results after measuring hair growth on the 21st day, where the results were 11.83+0.06 mm for positive control, 1.71+0.06 mm as blank, 1.69+0.06 mm for the group without treatment, and successively the results of the group given clove leaf extract 5, 10, and 15% were 4.00+0.06; 4.80+0.06; and 5.46+0.08 mm.
Conclusion: Hair tonics containing clove ethanol extract can increase rabbit hair growth.
2. Powell BC, Rogers, GE. Cyclic hair loss and regrowth in transgenic mice overexpressing an intermediate filament gene. EMBO J 1990;9:1485–93.
3. Phillips III JH, Smith SL, Storer JS. Hair loss: common congenital and acquired causes. Postgrad Med 1986;79:207–15.
4. Kapoor VP. Herbal cosmetics for skin and hair care. Indian J Nat Prod Resour 2005;4:306–14.
5. T Chiu CH, Huang SH, D Wang HM. A review: hair health, concerns of shampoo ingredients and scalp nourishing treatments. Curr Pharm Biotechnol 2015;16:1045–52.
6. Goluch Koniuszy ZS. Nutrition of women with hair loss problem during the period of menopause. Prz Menopauzalny 2016;15:56–61.
7. Dureja H, Khaushik D, Gupta M, Kumar V, Lather V. Cosmeceuticals: an emerging concept. Indian J Pharmacol 2005;37:155–9.
8. Baumann LS. Less known botanical cosmeceuticals. Dermatol Ther 2007;20:330–42.
9. Joshi LS, Pawar HA. Herbal cosmetics and cosmeceuticals: an overview. Nat Prod Chem Res 2015;3:1–8.
10. Shorter K, Farjo NP, Picksley SM, Randall VA. Human hair follicles contain two forms of ATP?sensitive potassium channels, only one of which is sensitive to minoxidil. FASEB J 2008;22:1725–36.
11. Sawaya ME, Shapiro J. Androgenetic alopecia: new approved and unapproved treatment. Dermatol Clin 2000;18:47–61.
12. Savin RC, Atton AV. Minoxidil: update on its clinical role. Dermatol Clin 1993;11:55–64.
13. Mittal M, Gupta N, Parashar P, Mehra V, Khatri M. Phytochemical evaluation and pharmacological activity of Syzygium aromaticum: a comprehensive review. Int J Pharm Pharm Sci 2014;6:67-72.
14. Chomchalow N. Spice production in asia-an overview. AU J Thechnol 2001;5:27–8.
15. Prajneshu M, Gupta VK. Patenting on clove. J Intellect Prop Rights 2002;7:24–32.
16. Kaur J, Kaur S, Mahajan A. Herbal medicines: possible risks and benefits. Am J Phytomed Clin Ther 2013;1:226–39.
17. Gulcin I, Elmastas M, Aboul Enein HY. Antioxidant activity of clove oil–a powerful antioxidant source. Arab J Chem 2012;5:489–99.
18. Abo El-Maati M, Mahgoub SA, Labib SM, Al-Gaby AMA, Ramadan MF. Phenolic extracts of clove (Syzygium aromaticum) with novel antioxidant and antibacterial activities. Eur J Integr Med 2016;8:494–504.
19. Fitrilia T, Bintang M, Safithri M. Phytochemical screening and antioxidant activity of clove mistletoe leaf extracts (Dendrophthoe pentandra (L.) Miq). IOSR J Pharm 2015;5:13–8.
20. Nassar MI, Gaara AH, El-Ghorab AH, Farrag A-RH, Shen H, Huq E, et al. Chemical constituents of clove (Syzygium aromaticum, fam. myrtaceae) and their antioxidant activity. Rev Latinoamer Quin 2007;35:47–57.
21. Kasai H, Shirao M, Ikegami Kawai M. Analysis of volatile compounds of clove (Syzygium aromaticum) buds as influenced by growth phase and investigation of antioxidant activity of clove extracts. Flavour Fragr J 2015;31:178–84.
22. Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015;4:1-6.
23. Lemaire C, Plenevaux A, Aerts J, Del Fiore G, Brihaye C, Le Bars D, et al. Solid phase extraction-an alternative to the use of rotary evaporators for solvent removal in the rapid formulation of PET radiopharmaceuticals. J Label Compd Radiopharm J Int Isot Soc 1999;42:63–75.
24. Nanda B, Lina S, Shivam L. Formulation and evaluation of herbal hair tonic. Res J Top Cosmet Sci 2015;6:86–90.
25. Patil A, Bhide S, Bookwala M, Soneta B, Shankar V, Almotairy A, et al. Stability of organoleptic agents in pharmaceuticals and cosmetics. AAPS PharmSciTech 2018;19:36–47.
26. Kusuma SAF, Abdassah M, Valas BE. Formulation and evaluation of anti acne gel containing Citrus Auratifolia fruit juice using carbopol as gelling agent. Int J Appl Pharm 2018;10:147-52.
27. Chen XQ, Antman MD, Gesenberg C, Gudmundsson OS. Discovery pharmaceutics-challenges and opportunities. AAPS J 2006;8:E402–8.
28. Tanaka S, Saiyo M, Tabata M. Bioassay of crude drugs for hair growth promoting activity in mice by a new simple method. Planta Med 1980;40:84–90.
29. Roy RK, Thakur M, Dixit VK. Development and evaluation of polyherbal formulation for hair growth–promoting activity. J Cosmet Dermatol 2007;6:108–12.
30. Hwang SL, Kim JC. In vivo hair growth promotion effects of cosmetic preparations containing hinokitiol-loaded poly(?-caprolacton) nanocapsules. J Microencapsul 2008;25:351–6.
31. Wendtner S, Korting HC. The pH of the skin surface and its impact on the barrier function. Ski Pharmacol Appl Ski Physiol 2006;19:296–302.
32. Soppimath KS, Kulkarni AR, Aminabhavi TM. Chemically modified polyacrylamide-g-guar gum-based crosslinked anionic microgels as pH-sensitive drug delivery systems: preparation and characterization. J Controlled Release 2001;75:331–45.
33. Messenger AG, Rundegren J. Minoxidil: mechanisms of action on hair growth. Br J Dermatol 2004;150:186–94.
34. Patel S, Sharma V, Chauhan NS, Thakur M, Dixit VK. Hair growth: focus on herbal therapeutic agent. Curr Drug Discovery Technol 2015;12:21–42.
35. Mustarichia R, Gozali D, Wicaksono IA, Nafis FDR. A comparison study of hair tonic and gel formulation or Angiopteris evecta as a hair growth stimulant. Int J Appl Pharm 2019;11:199-205.
36. Kumar N, Chaiyasut C. Hair growth promoting activity of Carthamus tinctorius florets extract-loaded nanostructured lipid carriers. Int J Pharm Pharm Sci 2015;7:252-7.
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