The HAIR TONIC FORMULATION OF CLOVE LEAVES (Syzygium aromaticum) ETHANOL EXTRACT AND THE EFFECTIVENESS ON RABBIT HAIR GROWTH

  • Hafizhatul Abadi Institut Kesehatan Helvetia

Abstract

Objective: The purpose of this study was to determine the physical stability and effectiveness of hair growth in hair tonic preparations containing ethanol extract of clove leaves (Syzygium aromaticum) with a concentration variation of 5, 10, and 15%. Methods: Formulations were evaluated including organoleptic observation, pH checking, and preparation stability tests using the Climatic Chamber. The effectiveness of clove leaf extract as a hair tonic was tested by looking at hair growth in test animals. Results: The results showed that the preparation was stable at high temperatures (40 ± 2 oC) where the odor, color, clarity, and pH remained constant. Effectiveness test on rabbit hair growth showed significantly different results after measuring hair growth on the 21st day, where the results were 11.83 + 0.06 mm for positive control, 1.71 + 0.06 mm as blank, 1.69 + 0.06 mm for the group without treatment, and successively the results of the group given clove leaf extract 5, 10, and 15% were 4.00 + 0.06; 4.80 + 0.06; and 5.46 + 0.08 mm. Conclusion: Hair tonics containing clove ethanol extract can increase rabbit hair growth.

Keywords: ethanol extract, hair tonic, Syzygium aromaticum

References

1. Randall VA. Androgens and Hair Growth. Dermatol Ther [Internet]. 2008;21(5):314–28. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1529-8019.2008.00214.x
2. Powell BC, Rogers, G E. Cyclic Hair?Loss and Regrowth in Transgenic Mice Overexpressing an Intermediate Filament Gene. EMBO J. 1990;9:1485–93.
3. Phillips III JH, Smith SL, Storer JS. Hair loss: Common Congenital and Acquired Causes. Postgrad Med. 1986;79(5):207–15.
4. Kapoor VP. Herbal Cosmetics for Skin and Hair care. Indian J Nat Prod Resour. 2005;4(4):306–14.
5. T. Chiu CH, Huang SH, D. Wang HM. A Review: Hair Health, Concerns of Shampoo Ingredients and Scalp Nourishing Treatments. Curr Pharm Biotechnol. 2015;16(12):1045–52.
6. Goluch-Koniuszy ZS. Nutrition of Women with Hair Loss Problem during the Period of Menopause. Prz Menopauzalny. 2016;15(1):56–61.
7. Dureja H, Khaushik D, Gupta M, Kumar V, Lather V. Cosmeceuticals: An Emerging Concept. Indian J Pharmacol. 2005;37(3):155–9.
8. Baumann LS. Less?Known Botanical Cosmeceuticals. Dermatol Ther. 2007;20(5):330–42.
9. Joshi LS, Pawar HA. Herbal Cosmetics and Cosmeceuticals: An Overview. Nat Prod Chem Res. 2015;3(2):1–8.
10. Shorter K, Farjo NP, Picksley SM, Randall VA. Human Hair Follicles Contain Two Forms of ATP? Sensitive Potassium Channels, only One of which is Sensitive to Minoxidil. FASEB J. 2008;22(6):1725–36.
11. Sawaya ME, Shapiro J. Androgenetic Alopecia: New Approved and Unapproved Treatment. Dermatol Clin. 2000;18(1):47–61.
12. Savin RC, Atton A V. Minoxidil: Update on Its Clinical Role. Dermatol Clin. 1993;11(1):55–64.
13. Batiha GE-S, Alkazmi LM. Syzygium aromaticum L. (Myrtaceae): Traditional Uses, Bioactive Chemical Constituents, Pharmacological and Toxicological Activities. Wasef, Lamiaa G Beshbishy, Amany Magdy Nadwa Eman H Rashwan, Eman K. 2020;10(2):202.
14. Chomchalow N. Spice Production in Asia - An Overview. AU J Thechnology. 2001;5(1):27–8.
15. Prajneshu M, Gupta VK. Patenting on Clove. J Intellect Prop Rights. 2002;7(1):24–32.
16. Kaur J, Kaur S, Mahajan A. Herbal Medicines: Possible Risks and Benefits. Am J Phytomedicine Clin Ther. 2013;1(2):226–39.
17. Gulcin I, Elmastas M, Aboul-Enein HY. Antioxidant Activity of Clove Oil – A Powerful Antioxidant Source. Arab J Chem. 2012;5(4):489–99.
18. Abo El-Maati M, Mahgoub SA, Labib SM, Al-Gaby AMA, Ramadan MF. Phenolic Extracts of Clove (Syzygium aromaticum) with Novel Antioxidant and Antibacterial Activities. Eur J Integr Med. 2016;8(4):494–504.
19. Fitrilia T, Bintang M, Safithri M. Phytochemical Screening and Antioxidant Activity of Clove Mistletoe Leaf Extracts (Dendrophthoe pentandra (L.) Miq). IOSR J Pharm. 2015;5(8):13–8.
20. Nassar MI, Gaara AH, El-Ghorab AH, Farrag A-RH, Shen H, Huq E, et al. Chemical Constituents of Clove (Syzygium aromaticum, Fam. Myrtaceae) and Their Antioxidant Activity. Rev Latinoamer Quin. 2007;35(3):47–57.
21. Kasai H, Shirao M, Ikegami-Kawai M. Analysis of Volatile Compounds of Clove (Syzygium aromaticum ) Buds as Influenced by Growth Phase and Investigation of Antioxidant Activity of Clove Extracts. Flavour Fragr J. 2015;31(2):178–84.
22. Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants. 2015;4(196):412–2167.
23. Lemaire C, Plenevaux A, Aerts J, Del Fiore G, Brihaye C, Le Bars D, et al. Solid phase extraction—an alternative to the use of rotary evaporators for solvent removal in the rapid formulation of PET radiopharmaceuticals. J Label Compd Radiopharm Off J Int Isot Soc. 1999;42(1):63–75.
24. Nanda B, Lina S, Shivam L. Formulation and Evaluation of Herbal Hair Tonic. Res J Top Cosmet Sci. 2015;6(2):86–90.
25. Patil A, Bhide S, Bookwala M, Soneta B, Shankar V, Almotairy A, et al. Stability of Organoleptic Agents in Pharmaceuticals and Cosmetics. AAPS PharmSciTech. 2018;19:36–47.
26. Chen X-Q, Antman MD, Gesenberg C, Gudmundsson OS. Discovery Pharmaceutics—Challenges and Opportunities. AAPS J. 2006;8:E402–8.
27. Tanaka S, Saiyo M, Tabata M. Bioassay of Crude Drugs for Hair Growth Promoting Activity in Mice by a New Simple Method. Planta Med. 1980;40:84–90.
28. Roy RK, Thakur M, Dixit VK. Development and Evaluation of Polyherbal Formulation for Hair Growth–Promoting Activity. J Cosmet Dermatol. 2007;6(2):108–12.
29. Hwang SL, Kim JC. In Vivo Hair Growth Promotion Effects of Cosmetic Preparations Containing Hinokitiol-Loaded Poly(?-caprolacton) Nanocapsules. J Microencapsul. 2008;25(5):351–6.
30. Wendtner S, Korting HC. The pH of the Skin Surface and Its Impact on the Barrier Function. Ski Pharmacol Appl Ski Physiol. 2006;19(6):296–302.
31. Soppimath KS, Kulkarni AR, Aminabhavi TM. Chemically Modified Polyacrylamide-g-guar Gum-Based Crosslinked Anionic Microgels as pH-Sensitive Drug Delivery Systems: Preparation and Characterization. J Control Release. 2001;75(3):331–45.
32. Messenger AG, Rundegren J. Minoxidil: Mechanisms of Action on Hair Growth. Br J Dermatol. 2004;150(2):186–94.
33. Patel S, Sharma V, Chauhan NS, Thakur M, Dixit VK. Hair Growth: Focus on Herbal Therapeutic Agent. Curr Drug Discov Technol. 2015;12(1):21–42.
Statistics
3 Views | Downloads
How to Cite
Abadi, H. (2020). The HAIR TONIC FORMULATION OF CLOVE LEAVES (Syzygium aromaticum) ETHANOL EXTRACT AND THE EFFECTIVENESS ON RABBIT HAIR GROWTH. International Journal of Applied Pharmaceutics, 12(6). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39027
Section
Original Article(s)