CHEMOMETRIC ASSISTED QUANTITATIVE DETERMINATION OF VITEXIN IN ETHANOLIC EXTRACT OF BINAHONG (ANREDERA CORDIFOLIA (TEN.) STEENIS) LEAVES

  • Sri Hartati Yuliani Sanata Dharma University
  • OCTAVIANUS BUDI SANTOSA Faculty of Pharmacy, Sanata Dharma University
  • OCTAVIANUS BUDI SANTOSA Faculty of Pharmacy, Sanata Dharma University
  • Michael Raharja Gani Faculty of Pharmacy, Sanata Dharma University
  • Michael Raharja Gani Faculty of Pharmacy, Sanata Dharma University

Abstract

Objective: The objective of this study was to develop UV spectroscopy method in combination with multivariate analysis for determining vitexin in binahong (Anredera cordifolia (Ten.) Steenis) leaves extract.


 


Methodology: The partial least square (PLS) regression and the principal component regression (PCR) was performed in this study to evaluate several statistical performances such as coefficient of determination (R2), root mean square error of calibration (RMSEC), root mean square error of cross-validation (RMSECV), root mean square error of prediction (RMSEP) and relative error of prediction (REP). Cross validation in this study was performed using leave one out technique.


 


Results: The R2 values of calibration data sets resulted from PLS ​​and PCR method were 0.9675 and 0.9648, respectively. The low values of RMSEC and RMSECV both for PLS ​​and PCR method indicated the minimum error of the calibration models. The R2 values of validation data sets resulted from PLS ​​and PCR method were 0.9778 and 0.9820, respectively. The low values of RMSEP both for PLS ​​and PCR method indicated the minimum error of prediction generated from the calibration data sets. Multivariate calibration techniques were applied to determine content of vitexin in binahong leaves extract. Predicted values from the multivariate calibration models were compared to the actual values determined from a validated HPLC method. It was found that PLS models resulted the lowest REP values compared to the PCR models.


 


Conclusion: The chemometrics technique can be applied as alternative method for determining vitexin levels in the ethanol solution of binahong leaves extract.


 


KEYWORDS : Binahong, HPLC, UV Spectroscopy, Vitexin.

Keywords: Binahong, HPLC, UV Spectroscopy, Vitexin

References

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Yuliani, S. H., SANTOSA, O. B., SANTOSA, O. B., Gani, M. R., & Gani, M. R. (2020). CHEMOMETRIC ASSISTED QUANTITATIVE DETERMINATION OF VITEXIN IN ETHANOLIC EXTRACT OF BINAHONG (ANREDERA CORDIFOLIA (TEN.) STEENIS) LEAVES. International Journal of Applied Pharmaceutics, 12(6). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39248
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