In-vitro evaluation of lectinized cisplatin bearing liposomes system

In-vitro evaluation of lectinized cisplatin bearing liposomes system

  • Rahul Tiwari Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, Madhya Pradesh-470003, India
  • Kaliyaperumal Viswanathan Translational Research Platform for Veterinary Biologicals (TRPVB), Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 051, Tamil Nadu, India.
  • Suresh Prasad Vyas Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, Madhya Pradesh-470003, India
  • Vandana Soni Dr.H.S.Gour University, Sagar

Abstract

Aim: The purpose of this study was to evaluate the extent and mechanism anti-cancer drug loaded liposomes using wheat germ agglutinin as a guiding molecule.


Method: For the drug loaded liposome synthesis, the thin film hydration method was used and the drug cisplatin was loaded during the synthesis and followed by the surface modification using wheat germ agglutinin (WGA) lectin.  The developed system was confirmed based on transmission electron microscopy (TEM), atomic force microscopy (AFM), particle size (PS) analyzer, polydispersity index and Zeta Potential analyzer.


Results: The TEM based studies indicated that the size of the liposome was 200 ± 5 nm.  The Ex- vivo study was conducted using the MCF-7 cancer cell line. The drug release indicated that the developed liposome release the drug in sustained manner.


Conclusion: The MTT studies indicated that the drug molecules were initially get delivered to the inside the cell. This formulation offered new simple approach and effectively kill the cells via targeting the nucleus. 

Keywords: Liposomes; Wheat germ agglutinin; Cisplatin; MCF-7 cells; Nucleus targeting

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Tiwari, R., Viswanathan, K., Vyas, S. P., & Soni, V. (2020). In-vitro evaluation of lectinized cisplatin bearing liposomes system . International Journal of Applied Pharmaceutics, 12(6). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39350
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