IN VITRO EVALUATION OF LECTINIZED CISPLATIN BEARING LIPOSOMES SYSTEM

  • RAHUL TIWARI Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, Madhya Pradesh 470003, India
  • KALIYAPERUMAL VISWANATHAN Translational Research Platform for Veterinary Biologicals (TRPVB), Tamil Nadu Veterinary and Animal Sciences University, Chennai 600051, Tamil Nadu, India
  • SURESH PRASAD VYAS Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, Madhya Pradesh 470003, India
  • VANDANA SONI Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, Madhya Pradesh 470003, India

Abstract

Objective: The purpose of this study was to evaluate the extent and mechanism anti-cancer drug-loaded liposomes using wheat germ agglutinin as a guiding molecule.


Methods: For the drug-loaded liposome synthesis, the thin film hydration method was used and the drug cisplatin was loaded during the synthesis and followed by the surface modification using wheat germ agglutinin (WGA) lectin. The developed system was confirmed based on transmission electron microscopy (TEM), atomic force microscopy (AFM), particle size (PS) analyzer, polydispersity index and Zeta Potential analyzer.


Results: The results showed the surface modified by liposomes had the particle size 200±5 nm. The wheat germ agglutinin coated on the surface to liposome led to a reduction in zeta potential and drug entrapment efficiency while particle size increased. Plain liposomes containing cisplatin had less effect than WGA modified liposome on MCF-7 cell lines.


Conclusion: The MTT studies indicated that the drug molecules were initially get delivered to the inside the cell. This formulation offered new simple approach and effectively kill the cells via targeting the nucleus.

Keywords: Liposomes, Wheat germ agglutinin, Cisplatin, MCF-7 cells, Nucleus targeting

References

1. Siddik ZH. Cisplatin: mode of cytotoxic action and molecular basis of resistance. Oncogene 2003;22:7265-79.
2. Wong PT, Choi SK. Mechanisms of drug release in nanotherapeutic delivery systems. Chem Rev 2015;115:3388-432.
3. Dasari S, Tchounwou PB. Cisplatin in cancer therapy: molecular mechanisms of action. Eur J Pharmacol 2014;740:364-78.
4. Mechref Y, Novotny MV. Structural investigations of glycoconjugates at high sensitivity. Chem Rev 2002;102:321-70.
5. Finlay DR, Newmeyer DD, Price TM, Forbes DJ. Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores. J Cell Biol 1987;104:189-200.
6. Bangham A, Standish MM, Watkins JC. Diffusion of univalent ions across the lamellae of swollen phospholipids. J Mol Biol 1965;13:238-IN227.
7. Bogdanov A, Klibanov A, Torchilin V. Protein immobilization on the surface of liposomes via carbodiimide activation in the presence of N?hydroxysulfosuccinimide. FEBS Lett 1988;231:381-4.
8. Damari SP, Shamrakov D, Varenik M, Koren E, Nativ Roth E, Barenholz Y, Regev O. Practical aspects in size and morphology characterization of drug-loaded nano-liposomes. Int J Pharm 2018;547:648-55.
9. Spyratou E, Mourelatou EA, Makropoulou M, Demetzos C. Atomic force microscopy: a tool to study the structure, dynamics and stability of liposomal drug delivery systems. Expert Opin Drug Del 2009;6:305-17.
10. Tatulian SA. Structural characterization of membrane proteins and peptides by FTIR and ATR-FTIR spectroscopy. Methods Mol Biol 2013;974:177-218.
11. Fry DW, White JC, Goldman ID. Rapid separation of low molecular weight solutes from liposomes without dilution. Anal Biochem 1978;90:809-15.
12. Basotra M, Singh SK, Gulati M. Development and validation of a simple and sensitive spectrometric method for estimation of cisplatin hydrochloride in tablet dosage forms: application to dissolution studies. ISRN Anal Biochem 2013. https://doi.org/10.1155/2013/936254
13. Velazquez FN, Miretti M, Baumgartner MT, Caputto BL, Tempesti TC, Prucca CG. Effectiveness of ZnPc and of an amine derivative to inactivate glioblastoma cells by photodynamic therapy: an in vitro comparative study. Sci Rep 2019;9:3010.
14. Surnar B, Sharma K, Jayakannan M. Core–shell polymer nanoparticles for prevention of GSH drug detoxification and cisplatin delivery to breast cancer cells. Nanoscale 2015;7:17964-79.
15. Khare P, Jain A, Jain NK, Soni V, Jain SK. Glutamate-conjugated liposomes of dopamine hydrochloride for effective management of parkinsonism's. PDA J Pharm Sci Technol 2009;63:372-9.
16. Geng L, Osusky K, Konjeti S, Fu A, Hallahan D. Radiation-guided drug delivery to tumor blood vessels results in improved tumor growth delay. J Controlled Release 2004;99:369-81.
17. Athmakur H, Kondapi AK. Carmustine loaded lactoferrin nanoparticles demonstrates an enhanced antiproliferative activity against glioblastoma in vitro. Int J Appl Pharm 2018;10:234-41.
18. Maruyama K. Intracellular targeting the delivery of liposomal drugs to solid tumors based on EPR effects. Adv Drug Delivery Rev 2011;63:161-9.
19. Wang Z, Tian Y, Zhang H, Qin Y, Li D, Gan L, et al. Using hyaluronic acid-functionalized pH stimuli-responsive mesoporous silica nanoparticles for targeted delivery to CD44-overexpressing cancer cells. Int J Nanomed 2016;11:6485.
20. Mudakavi RJ, Raichur AM, Chakravortty D. Lipid coated mesoporous silica nanoparticles as an oral delivery system for targeting and treatment of intravacuolar salmonella infections. RSC Adv 2014;4:61160-6.
21. Bonnin S, Besson F, Gelhausen M, Chierici S, Roux B. A FTIR spectroscopy evidence of the interactions between wheat germ agglutinin and N?acetylglucosamine residues. FEBS Lett 1999;456:361-4.
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TIWARI, R., VISWANATHAN, K., VYAS, S. P., & SONI, V. (2020). IN VITRO EVALUATION OF LECTINIZED CISPLATIN BEARING LIPOSOMES SYSTEM. International Journal of Applied Pharmaceutics, 12(6), 60-64. https://doi.org/10.22159/ijap.2020v12i6.39350
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