DETERMINATION OF SPIROMESIFEN, QUINOLPHOS, MONOCROTOPHOS, CARBENDAZIM AND ACEPHATE RESIDUE BEHAVIOUR IN OKRA (ABELMOSHUS ESCULENTUS) BY LIQUID CHROMATOGRAPHY AND MASS SPECTROPHOTOMETRY AND THEIR DECONTAMINATION USING HOUSE HOLD PROCESSES
Objective: Field experiments were conducted at Agricultural University, Rajendra Nagar, Hyderabad, Telanagana to study the dissipation kinetics of carbendazim, monocrotophos, spiromesifen, acephate and quinalphos in okra fruit. Decontamination study was also conducted to evaluate quality of okra pods by reducing the residues of carbendazim, monocrotophos, spiromesifen, acephate and quinalphos by using different processes such as 2% salt solution, acetic acid, biowash, butter milk, cooking, drying, Formula 1(T7), frying, lemon water, sodium bicarbonate, tamarind water and tap water.
Methods: All the pesticide residues with one test dose at two spray i.e., first spray at flowering stage and second spray after an interval of ten days was carried out. The samples drawn at specific periods were analyzed by liquid chromatography and mass spectrophotometry (LC-MS/MS).
Results: The initial deposit of carbendazim, monocrotophos, spiromesifen, acephate and quinalphos in okra was found to be 2.239, 2.586, 2.401, 1.39, 0.78 mg/kg respectively. More than 98 % of carbendazim, spirofesifin, acephate and quinolphos dissipated after 15 d and monocrotophos was dissipated after 10 d. Sodium bicarbonate and 2% salt solution are the best methods for decontamination after cooking. The decontamination values of frying and formula 1 seems to be almost same. After these two methods biowash thought to be the method of choice.
Conclusion: These results are helpful in setting up maximum residual limit (MRLs) of these pesticides in okra in India. From the results, it could be recommended that cooking suits best for almost all of the pesticide residues.
2. Sardana HR. Integrated pest management in vegetables. In: Training manual-2, Training on IPM for Zonal Agricultural Research Stations; 2001. p. 105-18.
3. Kumari B, Kumar R, Madan VK, Rajvir S, Jagdeep S, Kathpal TS. Magnitude of pesticidal contamination in winter vegetables from Hisar, Haryana. Environ Monit Assess 2003;87:311-8.
4. Rahmiani NH, Suryadi H. Analysis of fenpropathrin, lambda-cyhalothrin, and chlorothalonil in potato and tomato samples using gas chromatography with an electron capture detector. Int J Appl Pharm 2018;10:70-5.
5. Wentzell J, Cassar M, Kretzschmar D. Organophosphate-Induced changes in the PKA regulatory function of swiss cheese/NTE lead to behavioural deficits and neurodegeneration. PLoS One 2014;9:87526.
6. Sharma RK, Goyal AK. Agro-pesticides and andrology. Int J Pharm Pharm Sci 2014;6:12-9.
7. Janssens L, Stoks R. Fitness effects of chlorpyrifos in the damselfly enallagma cyathigerum strongly depend upon temperature and food level and can bridge metamorphosis. PLoS One 2013;8:68107.
8. Canesi L, Negri A, Barmo C, Banni M, Gallo G. The organophosphate chlorpyrifos interferes with the responses to 17b-estradiol in the digestive gland of the marine mussel mytilus galloprovincialis. PLoS One 2011;6:19803.
9. Sasikala C, Jiwal S, Rout P, Ramya M. Biodegradation of chlorpyrifos by bacterial consortium isolated from agriculture soil. World J Microb Biot 2012;28:1301.
10. Trunnelle KJ, Bennett DH, Tulve NS, Clifton MS, Davis MD. Urinary pyrethroid and chlorpyrifos metabolite concentrations in northern California families and their relationship to indoor residential insecticide levels, part of the study of use of products and exposure-related behaviour (SUPERB). Environ Sci Technol 2014;48:1931-9.
11. Watts M. Chlorpyrifos as a possible global persistent organic pollutant. Pesticide Network North America, Oakland, CA, USA; 2012. p. 17.
12. Popp J, Peto K, Nagy J. Pesticide productivity and food security. A review. Agron Sustain Dev 2013;33:243-55.
13. Zhang NN, Liu CF, Yang F, Dong SL, Han ZJ. Resistance mechanisms to chlorpyrifos and F392W mutation frequencies in the acetylcholine esterase ace1allele of field populations of the tobacco whitefly, Bemisia tabaci in China. J Insect Sci 2012;12:41.
14. Ouyang Y, Chueca P, Scott SJ, Montez GH, Grafton Cardwell EE. Chlorpyrifos bioassay and resistance monitoring of san joaquin valley california citricola scale populations. J Econ Entomol 2010;103:1400-4.
15. Garau VL, Angioni A, Aguilera Del Real A, Russo MT, Cabras P. Disappearance of azoxystrobin, cyprodinil, and fludioxonil on tomato in a greenhouse. J Agric Food Chem 2002;50:1929-32.
16. Cabras P, Meloni M, Manca MR, Pirisi FM, Cabitza F. Pesticide residues in lettuce. 1. Influence of the cultivar. J Agric Food Chem 1988;36:92-5.
17. Wang M, Zhang Q, Cong L, Yin W, Wang M. Enantioselective degradation of metalaxyl in cucumber, cabbage, spinach and pakchoi. Chemosphere 2014;95:241-56.
18. Sun H, Xu J, Yang S, Liu G, Dai S. Plant uptake of aldicarb from contaminated soil and its enhanced degradation in the rhizosphere. Chemosphere 2004;54:569-74.
19. Fan S, Zhang F, Deng K, Yu C, Liu S. Spinach or amaranth contains highest residue of metalaxyl, fluazifop-p-butyl, chlorpyrifos, and lambda-cyhalothrin on six leaf vegetables upon open field application. J Agric Food Chem 2013;61:2039-44.
20. Montemurro N, Grieco F, Lacertosa G, Visconti A. Chlorpyrifos decline curves and residue levels from different commercial formulations applied to oranges. J Agric Food Chem 2002;50:5975-80.
21. Cabras P, Meloni M, Gennari M, Cabitza F, Ubeddu M. Pesticide residues in lettuce. 2. Influence of formulations. J Agric Food Chem 1989;37:1405-7.
22. Angioni A, Schirra M, Garau VL, Melis M, Tuberoso CIG, Cabras P. Residues of azoxystrobin, fenhexamid and pyrimethanil in strawberry following field treatments and the effect of domestic washing. Food Addit Contam 2004;21:1065-70.
23. Ling Y, Wang H, Yong W, Zhang F, Sun L, Yang ML, et al. The effects of washing and cooking on chlorpyrifos and its toxic metabolites in vegetables. Food Control 2011;22:54-8.
24. Chandra S, Kumar M, Mahindrakar AN, Shinde LP. Effects of household processing on reduction of pesticide residues in Brinjal and Okra. Int J Adv Pharm Biol Chem 2015;4:98-102.
25. Balinova AM, Mladenova RI, Shtereva DD. Effects of processing on pesticide residues in peaches intended for baby food. Food Addit Contam 2006;23:895-901.
26. Kumari B. Effects of household processing on reduction of pesticide residues in vegetables. Int J Agric Biol 2008;3:46-51.
27. Kaushik G, Satya S, Naik SN. Food processing a tool to pesticide residue dissipation–a review. Food Res Int 2009;42:26-40.
28. Document No SANCO/12571/2013 European Union. 2013. Method validation and quality control procedures for pesticides residues analysis in food and feed. Available from: http://ec.europa.eu/food/plant/protection/pesticides/docs/qualcontrol_en.pdf. [Last accessed on 04 Nov 2012.
29. U. S. FDA, U. S. Department of Health and Human Services Food and Drug Administration. Guidance for Industry Q2B Validation of Analytical Procedures: Methodology. Rockville MD; 1996.
30. Walfish S. Analytical methods: a statistical perspective on the ICH Q2A and Q2B guidelines for validation of analytical methods. BioPharm Int 2006. p. 1-6.
31. Assis EC, Silva AA, Barbosa LC, Queiroz MELR, Dantonino L, Gonçalves VA. Optimization and validation of the solid-liquid extraction technique for determination of picloram in soils by high performance liquid chromatography. Planta Daninha Viçosa MG 2011;29:683-96.
32. Nagesh M, Verma S. Decontamination of cabbage treated with chlorpyriphos and quinalphos. Ind J Entomol 1997;59:404-10.
33. Nath G, Jat RN, Srivastava BP. Effect of washing, cooking and dehydration on the removal of some Insecticides from Okra (Abelmoschus esculentus Moench.). Int J Food Sci Tech 1975;12:127-30.
34. Kadian S, Kumar R, Grewal RB, Srivastava SP. Effect of household processing on cypermethrin residues in some commonly used vegetables. Pestology 2001;25:10-3.
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