THE EXPRESSION OF NESTIN IN THE INDUCED DIFFERENTIATION INTO NEURONS OF RAT BONE MARROW MESENCHYMAL STEM CELLS BY NEUROTROPHIN-3 (NT-3)

  • ARIYANI NOVIANTARI National Institute of Health Research and Development, Ministry of Health, Republic of Indonesia, Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • RADIANA DHEWAYANI ANTARIANTO Department Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia, Stem Cell and Tissue Engineering Research Cluster, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • LUTFAH RIF’ATI National Institute of Health Research and Development, Ministry of Health, Republic of Indonesia
  • RATIH RINENDYAPUTRI National Institute of Health Research and Development, Ministry of Health, Republic of Indonesia
  • MASAGUS ZAINURI National Institute of Health Research and Development, Ministry of Health, Republic of Indonesia
  • FRANS DANY National Institute of Health Research and Development, Ministry of Health, Republic of Indonesia

Abstract

Objective: The aim of this study was to examine the role of NT-3 as a single neurotrophic factor in the expression of nestin in the neural differentiation of MSCs.


Methods: MSCs were isolated from rat bone marrow and induced with NT-3 at concentrations of 20, 25, and 30 ng/ml for 7 and 14 d (the control was no NT-3). Nestin underwent immunocytochemical analysis on days 7 and 14. Five high-power random fields were documented.


Results: A post-hoc analysis using LSD after one-way ANOVA test yielded a statistically significant difference in the percentage of nestin-positive cells in MSCs with NT-3 at concentrations of 20, 25, and 30 ng/ml for 7 d compared to the control group (p<0.05). The percentages of nestin-positive cells at concentrations of 20, 25, and 30 ng/ml, and in the control data on day 7 were 14.55±1.26%, 16.20±1.07%, 13.78±1.19%, and 9.81±0.79%, respectively. NT-3 at 25 ng/ml induced the highest MSCs neural differentiation on day 7 and remained constant until day 14.


Conclusion: NT-3 plays a role in the early stage of differentiating MSCs from rat bone marrow into neurons, with the optimal concentration being 25 ng/ml.

Keywords: Differentiation, Mesenchymal stem cells, Nestin, Neuron, Neurotrophin-3

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NOVIANTARI, A., ANTARIANTO, R. D., RIF’ATI, L., RINENDYAPUTRI, R., ZAINURI, M., & DANY, F. (2020). THE EXPRESSION OF NESTIN IN THE INDUCED DIFFERENTIATION INTO NEURONS OF RAT BONE MARROW MESENCHYMAL STEM CELLS BY NEUROTROPHIN-3 (NT-3). International Journal of Applied Pharmaceutics, 12(3). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39472
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