THE HEMATOLOGIC PROFILE IN THE ACUTE TOXICITY TEST OF COGON GRASS ROOTS ETHANOL EXTRACT IN WISTAR RATS

  • VANESSA AYU SUMIRAT Magister Study Program of Biotechnology, Post Graduate School, Universitas Padjadjaran, Bandung, Indonesia, Medical Analyst Program, Bakti Asih School of Analyst, Bandung, Indonesia
  • IRMA MELYANI PUSPITASARI Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia, Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran, Bandung, Indonesia
  • NENI ANGGRAENI Medical Analyst Program, Bakti Asih School of Analyst, Bandung, Indonesia
  • MAS RIZKY ANGGUN ADIPURNA SYAMSUNARNO Magister Study Program of Biotechnology, Post Graduate School, Universitas Padjadjaran, Bandung, Indonesia, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia, Central Laboratory, Universitas Padjadjaran, Bandung, Indonesia

Abstract

Objective: This study aimed to investigate the hematologic profile of Wistar rats in the acute toxicity test of Cogon grass-root ethanol extract (CGEE).


Methods: Cogon grassroots were dissolved in 70% ethanol. An acute toxicity test was conducted based on The National Agency of Drug and Food Control of the Republic of Indonesia. Five female rats in the treatment group were administered a single high dose of 5000 mg/kg body weight (BW) of CGEE in 200 μl of 0.5% carboxymethyl cellulose (CMC), and the 5 female rats in the control group were administered 200 μl of 0.5% CMC. After 14 d, blood samples were collected, and 18 hematologic parameters were measured with a hematology analyzer. Statistical analyses were performed to compare the parameters between the two groups with the independent t-test for normally distributed data and the Mann Whitney test for non-normally distributed data.


Results: None of the hematologic parameters in the treatment group significantly differed from those in the control group after 14 d of observation (P>0.05).


Conclusion: A single high dose of 5000 mg/kg BW of CGEE did not change the hematologic profile of Wistar rats. These results indicate that CGEE does not have an acute hemotoxic effect, at least for hematologic parameters.

Keywords: Acute Toxicity, Cogon Grass root Ethanol Extract, Hematologic Profiles

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SUMIRAT, V. A., PUSPITASARI, I. M., ANGGRAENI, N., & SYAMSUNARNO, M. R. A. A. (2020). THE HEMATOLOGIC PROFILE IN THE ACUTE TOXICITY TEST OF COGON GRASS ROOTS ETHANOL EXTRACT IN WISTAR RATS. International Journal of Applied Pharmaceutics, 12(3). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39478
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