THE HEMATOLOGIC PROFILE IN THE ACUTE TOXICITY TEST OF COGON GRASS ROOTS ETHANOL EXTRACT IN WISTAR RATS
Objective: This study aimed to investigate the hematologic profile of Wistar rats in the acute toxicity test of Cogon grass-root ethanol extract (CGEE).
Methods: Cogon grassroots were dissolved in 70% ethanol. An acute toxicity test was conducted based on The National Agency of Drug and Food Control of the Republic of Indonesia. Five female rats in the treatment group were administered a single high dose of 5000 mg/kg body weight (BW) of CGEE in 200 μl of 0.5% carboxymethyl cellulose (CMC), and the 5 female rats in the control group were administered 200 μl of 0.5% CMC. After 14 d, blood samples were collected, and 18 hematologic parameters were measured with a hematology analyzer. Statistical analyses were performed to compare the parameters between the two groups with the independent t-test for normally distributed data and the Mann Whitney test for non-normally distributed data.
Results: None of the hematologic parameters in the treatment group significantly differed from those in the control group after 14 d of observation (P>0.05).
Conclusion: A single high dose of 5000 mg/kg BW of CGEE did not change the hematologic profile of Wistar rats. These results indicate that CGEE does not have an acute hemotoxic effect, at least for hematologic parameters.
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