MUCOADHESIVE POLYMERIC FILMS OF ACYCLOVIR PRONIOSOMES FOR BUCCAL ADMINISTRATION

  • DEEKSHA U. SUVARNA Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Deralakatte, Mangalore 575018, Karnataka, India
  • MARINA KOLAND Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Deralakatte, Mangalore 575018, Karnataka, India http://orcid.org/0000-0003-2628-3582
  • ANANTH PRABHU Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Deralakatte, Mangalore 575018, Karnataka, India
  • SINDHOOR S. M. Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Deralakatte, Mangalore 575018, Karnataka, India

Abstract

Objective: The aim of the present work was to formulate and evaluate proniosomes of the poorly soluble drug, acyclovir incorporated in mucoadhesive polymeric films for improved buccal mucosal permeability of the drug while achieving prolonged release.


Methods: Acyclovir was formulated as proniosomes using Span 60 and cholesterol. The prepared proniosomes were loaded into mucoadhesive polymeric films prepared with varying quantities of carbopol 934P and HPMC K15M. The proniosome incorporated films were evaluated for physicomechanical characters, mucoadhesion, swelling index, drug content, in vitro drug release and ex vivo permeation through porcine buccal mucosa.


Results: Hydration of the proniosomes produced spherical vesicles or niosomes, which was confirmed by Scanning Electron Microscopy. The optimized formulation selected on the basis of vesicle size, entrapment efficiency PDI, Zetz potential and in vitro drug release was selected for incorporation into mucoadhesive polymeric films. All the films showed excellent physicomechanical characters. Formulations with higher proportions of carbopol produced slower in vitro drug release. The kinetics of release of drug from all the formulations appeared to be zero-order based on their regression coefficient values. Comparative evaluation of ex vivo permeation from niosomal and non-niosomal films indicated that the former demonstrated improved mucosal permeation and drug release was also sustained for the 8 h period.


Conclusion: Mucoadhesive films impregnated with acyclovir loaded proniosomes could be a potential approach for buccal delivery of acyclovir for improving its absorption and bioavailability. 

Keywords: Cholesterol, Carbopol, Mucoadhesive, Proniosomes, Buccal, Permeation

References

1. Tripathi KD. Essentials of medical pharmacology. 6thed. New Delhi: JaypeeBrothers’s Medical Publishers (P) Ltd; 2008.
2. Drug Bank; 2012. Available from:http://www.drugbank.ca/drugs/DB00787 [Last accessed on 01 Aug 2020].
3. Johnson GL, Limon L, Trikha G, Wall H. Acute renal failure and neurotoxicity following oral acyclovir. Ann Pharmacother 1994;28:460-3.
4. Cortesi R, Esposito E. Acyclovir delivery systems. Expert Opin Drug Delivery 2008;5:1217–30.
5. Paul S, Kumar A, Yedurkar P, Sawant K. Design and development of multiple emulsion for enhancement of oral bioavailability of acyclovir. Drug DevInd Pharm 2013;39:1809–17.
6. Swarbrick J, Boylan JC.editors. Buccal Absorption of Drugs. Encyclopedia ofPharmaceutical Technology. Vol.2. New York: Marcel Dekker Inc.; 1990. p.189-208.
7. JianHwa G, Karsten C. Development of bioadhesivebuccal patches. In: Swarbrick J,Mathiowitz E. editors. Bioadhesivedrug delivery systems: fundamentals, novel approaches and development. 1sted. New York: Marcel Dekker Inc.; 1999. p. 541-59.
8. Rossi S, Sandri G, Ferrari F, Bonferoni M, Caramella C. Buccaldelivery of acyclovir from films based on chitosan and polyacrylic acid. Pharm DevTechnol 2003;8:199-208.
9. Degim T, Eglen B, OcakO. A sustained release dosage form of acyclovir for buccal application: An experimental study in dogs. J Drug Target2006;14:35-44.
10. Mahale N, Thakkar P, Mali R, Walunj D, Chaudhari S. Niosomes: novel sustained release nonionic stable vesicular systems-an overview. Adv Colloid Interface Sci 2012;183-184:46-54.
11. Chaw CS, Ah Kim KY. Effect of formulation compositions on niosomal preparations. Pharm DevTechnol 2013;18:667–72.
12. Yadav K, Yadav D, Mathur P, Syan N. Pronisomal gel: a provesicularappraoch for transdermal drug delivery. Der Pharm Lett 2010;2:189-98.
13. Mokhtar M,Sammour OA,Hammad MA,Megrab NA. Effect of some formulation parameters on flurbiprofen encapsulation and release rates of niosomes prepared from proniosomes. Int J Pharm 2008;361:4–11.
14. Mokale VJ, Ajit P, Priyanka P, Harshada I. Formulation and optimization of famotidine proniosomes: an in vitro and ex vivo study. J Expt Nano Sci 2016;11:54-65.
15. Viviane F, Safaa S, Allam AN. Proniosomes as a stable carrier for oral acyclovir: formulation and physicochemical characterization. J Am Sci 2012;8:417-27.
16. Benipal G. Design, development and evaluation of proniosomal gel of an antifungal drug–ketoconazole. Int J Pharm Sci Rev Res 2015;31:265-72.
17. Ram A, Thakur A, Mittal V. Pronisomalprovesicular system for transdermal delivery of hydralazine for hypertension. Asian J Pharm Clin Res 2012;5:219-25.
18. Sathali AAH, Rajalakshmi G. Evaluation of transdermal targeted niosomal drug delivery of terbinafine hydrochloride. Int J PharmTech Res 2010;2:2081–9.
19. Lohani A, Prasad N, Arya K. Formulation and characterization of mucoadhesivebuccal films of ranitidine hydrochloride. Int J PharmSci Res 2011;2:2457-62.
20. Satish Babu BK, Srinivasan BP. Preparation and evaluation of buccoadhesive films of atenolol. Ind J Pharm Sci 2008;70:175-9.
21. Koland M, Sandeep VP, Charyulu NR. Fast dissolving sublingual films of ondensetron hydrochloride effect of additives on in vitro drug release and mucosal permeation. J Young Pharm 2010;2:216-22.
22. Khanna R, Agrawal SP, Ahuja A. Preparation and evaluation of buccal films of clotrimazole for oral candida infections. Indian J Pharm Sci 1997;59:299-305.
23. Sang Chul Shin, JaYoung Kim. Enhanced permeation of triamcinolone acetonide through the buccal mucosa. Eur J Pharm Biopharm 2000;50:217-20.
24. Lohani A, Prasad N, Arya K. Formulation and characterization of mucoadhesivebuccal films of ranitidine hydrochloride. Int J Pharm Sci Res 2011;2:2457-62.
25. Mane PP, Bushetti SS, Keshavanti GG. Development and in vitroevaluation of mucoadhesivebuccal films of nebivolol. Indian J Pharm Sci 2014;76:166-9.
26. Lodhi M, Dubey A, Narayan R, Priya S. Formualtion and evaluation of buccal film of Ivabradine hydrochloride for the treatment of stable angina pectoris. Int J Pharm Investig 2013;3:47-53.
27. Nafee NA, Boraie NA, Ismail FA, Mortada LM. Design and characterization of mucoadhesivebuccal patches containing cetylpyridinium chloride. Acta Pharm 2003;53:199-212.
28. Fernandes F, Fortes A, da Cruz Fonseca S, Breitkreutz J, Ferraz H. Manufacture and characterization of mucoadhesivebuccal films based on pectin and gellan gum containing triamcinolone acetonide. Int J PolymSci 2018;2018:1-10.
29. Balakrishnana P, Shanmugama S, Leea WS, Leea WM, Kim JO. Formulation and in vitro assessment of minoxidilniosomes for enhanced skin delivery. Int J Pharm 2009;377:1–8.
30. Rogerson A, Cummings J, Florence AT. Adriamycin-loaded niosomes: drug entrapment, stability and release. J Microencapsul 1987;4:321–8.
31. Peh KK, Wong CF. Polymeric fims as vehicles for buccal delivery: swelling, mechanical and bioadhesiveproperties. J Pharm PharmSci 1999;2:53-61.
32. Anlar S, Capan Y, Hincal AA. Physico-chemical and bioadhesive properties of polyacrylic acid polymers. Pharmazie 1993;48:285–7.
33. Sharmin N, Al-Mamun MD, Jalil R. A novel method to study the effect of pH and excipients on water uptake and swelling behavior of carbopol polymers. Bangl Pharm J 2010;3:1–7.
34. Patel MM, Smart JD, Nevell TG, Ewen RJ, Eaton PJ, Tsibouklis J. Mucin/poly (acrylic acid) interactions: a spectroscopic investigation of mucoadhesion. Biomacromolecules 2003;4:1184-90.
35. Peppas NA. Analysis of fickian and non-fickian drug release from polymers. Pharm ActaHelv 1985;60:110-1.
36. Khan R, R Irchhaiya. In vitroin vivo evaluation of niosomal formulation of famotidine. Int J Pharm PharmSci2020;12:15-22.
37. Shah JC. Analysis of permeation data: evaluation of lag time method. Int J Pharm 1993;90:161–9.
38. Radha GV, Sastri KT, Prathyusha P, Bhanu P, Rajkumar J. Formulation and evaluation of aceclofenacproniosome loaded orabase for management of dental pain. Int J Appl Pharm 2018;10:204-10.
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SUVARNA, D. U., KOLAND, M., PRABHU, A., & S. M., S. (2021). MUCOADHESIVE POLYMERIC FILMS OF ACYCLOVIR PRONIOSOMES FOR BUCCAL ADMINISTRATION. International Journal of Applied Pharmaceutics, 13(1), 135-143. https://doi.org/10.22159/ijap.2021v13i1.39657
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