HPLC DETERMINATION OF SILDENAFIL IN TABLETS

  • MIGLENA SMERIKAROVA Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia
  • STANISLAV BOZHANOV Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, Sofia, Bulgaria
  • VANIA MASLARSKA Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, Sofia, Bulgaria

Abstract

Objective: The popularity of Sildenafil, the widespread distribution of various products and dietary supplements with added synthetic drugs, requires reliable analysis methods. This research study aimed to develop a simple isocratic HPLC method for the determination of Sildenafil in tablet dosage forms from the local market.


Methods: Separation was carried out at 30 °C, using column LiChrosorb® RP-18 (150 x 4.0 mm, 5 μm) with mobile phase consisting of acetonitrile: methanol: 0.5% triethylamine (15: 26: 59 v/v/v). The detector was set at 290 nm. The flow rate was 1.0 ml/min and the injection volume was 20 μl.


Results: Linear correlation was obtained within the range 6.25–50.0 μg/ml with correlation coefficient (R2) 0.9998. The achieved limits of detection and quantitation were 0.7 and 2.2 μg/ml, respectively.


Conclusion: The developed method can be applied for the quality control of Sildenafil preparations.

Keywords: Erectile dysfunction, Sildenafil, HPLC, Phosphodiesterase inhibitors, Tablets, Drugs

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SMERIKAROVA, M., BOZHANOV, S., & MASLARSKA, V. (2021). HPLC DETERMINATION OF SILDENAFIL IN TABLETS. International Journal of Applied Pharmaceutics, 13(1), 253-256. https://doi.org/10.22159/ijap.2021v13i1.39719
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