HPLC DETERMINATION OF SILDENAFIL IN TABLETS
Objective: The popularity of Sildenafil, the widespread distribution of various products and dietary supplements with added synthetic drugs require reliable methods
The aim of this research study was to develop a simple isocratic HPLC method for the determination of sildenafil in tablet dosage forms from the local market.
Methods: Separation was carried out at 30°C, using column LiChrosorb® RP-18 (150 x 4.0 mm, 5 μm) with mobile phase consisting of acetonitrile: methanol: 0.5%
triethylamine (15: 26: 59 v/v/v). The detector was set at 290 nm. The flow rate was 1.0 ml/min and injection volume was 20 μl.
Results: Linear correlation was obtained within the range 6.25 – 50.0 μg/ml with correlation coefficient (R2) 0.9998. The achieved limits of detection and
quantitation were 0.7 and 2.2 μg/ml respectively.
Conclusion: The developed method can be applied for quality control of Sildenafil preparations.
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