HPLC DETERMINATION OF SILDENAFIL IN TABLETS

  • Miglena Smerikarova Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia
  • Stanislav Bozhanov Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia
  • Vania Maslarska Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia

Abstract

ABSTRACT


Objective: The popularity of Sildenafil, the widespread distribution of various products and dietary supplements with added synthetic drugs require reliable methods


for analysis.


The aim of this research study was to develop a simple isocratic HPLC method for the determination of sildenafil in tablet dosage forms from the local market.


Methods: Separation was carried out at 30°C, using column LiChrosorb® RP-18 (150 x 4.0 mm, 5 μm) with mobile phase consisting of acetonitrile: methanol: 0.5%


triethylamine (15: 26: 59 v/v/v). The detector was set at 290 nm. The flow rate was 1.0 ml/min and injection volume was 20 μl.


Results: Linear correlation was obtained within the range 6.25 – 50.0 μg/ml with correlation coefficient (R2) 0.9998. The achieved limits of detection and


quantitation were 0.7 and 2.2 μg/ml respectively.


Conclusion: The developed method can be applied for quality control of Sildenafil preparations.

Keywords: Erectile dysfunction, Sildenafil, HPLC, Phosphodiesterase inhibitors, Tablets, Drugs
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Smerikarova, M., Bozhanov, S., & Maslarska, V. (2020). HPLC DETERMINATION OF SILDENAFIL IN TABLETS. International Journal of Applied Pharmaceutics, 13(1). Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/39719
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