IN LABORATORY GENERATION AND MATURATION OF HUMAN MONOCYTE-DERIVED DENDRITIC CELLS FOR CANCER IMMUNOTHERAPY

  • KANCHAN K. MISHRA Surat Raktadan Kendra and Research Centre, (Regional Blood Transfusion and Research Centre), (NABH Accredited and SIROs Recognition from DSIR, 1st Floor, Khatodara Health Centre, Khatodara 395002, Surat (Gujarat), India
  • SUMIT BHARADVA Surat Raktadan Kendra and Research Centre, (Regional Blood Transfusion and Research Centre), (NABH Accredited and SIROs Recognition from DSIR, 1st Floor, Khatodara Health Centre, Khatodara 395002, Surat (Gujarat), India
  • MEGHNAD G. JOSHI Department of Stem Cells and Regenerative Medicine, Centre for Interdisciplinary Research, D. Y. Patil Education Society Institution Deemed to be University, Kolhapur, India
  • ARVIND GULBAKE Department of Stem Cells and Regenerative Medicine, Centre for Interdisciplinary Research, D. Y. Patil Education Society Institution Deemed to be University, Kolhapur, India

Abstract

Dendritic cells (DCs) play a critical role in the regulation of adaptive immune responses, furthermore they act as a bridge between the innate and the adaptive immune systems they have been ideal candidates for cell-based immunotherapy of cancers and infections in humans. The first reported trial using DCs in 1995, since they have been used in trials all over the world for several of indications, including cancer and human immunodeficiency virus infection. Generally, for in vitro experiments or for DCs vaccination monocyte-derived dendritic cells (moDCs) were generated from purified monocytes that isolated from peripheral blood by density gradient centrifugation. A variety of methods can be used for enrichment of monocytes for generation of clinical-grade DCs. Herein we summarized up to date understanding of systems and inputs used in procedures to differentiate DCs from blood monocytes in vitro.

Keywords: Dendritic Cells, Monocyte-derived Dendritic Cells, Immunotherapy

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MISHRA, K. K., BHARADVA, S., JOSHI, M. G., & GULBAKE, A. (2020). IN LABORATORY GENERATION AND MATURATION OF HUMAN MONOCYTE-DERIVED DENDRITIC CELLS FOR CANCER IMMUNOTHERAPY. International Journal of Applied Pharmaceutics, 12(4), 46-52. https://doi.org/10.22159/ijap.2020.v12s4.40104
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