AUTHENTICATION OF SPRAGUE DAWLEY RATS (RATTUS NORVEGICUS) FAT WITH GC-MS (GAS CHROMATOGRAPHY-MASS SPECTROMETRY) COMBINED WITH CHEMOMETRICS
Objective: Misuse of rat meat as food worried people. Rat meat can come from research waste; one of the rats used in the research was the Sprague Dawley. Analysis of rat meat in food can be done using fat. The aim of this study was to authenticate rat fat with GC-MS combined with chemometrics.
Methods: The meat of Sprague Dawley rats, wild boar, goats, cow, and processed meatballs was put in the oven at 90-100 °C for±one hour, then derivatized with BF3 and NaOH in methanol to get the methyl ester for injected in GC-MS instrument. The results obtained were in the form of chromatograms and spectrograms. The data was processed using Principal Component Analysis (PCA) to grouping rat's meat with others (wild boars, goats, cows, and processed meatballs).
Results: Rat meat fatty acid analysis results with GC-MS were obtained oleate (43.32±1.43)%, linolenate (32.24±1.46)%, palmitate (19.75±0.09)%, palmitoleate (1.14±0.06)%, stearate (0.26±0.01)%, myristate(0.18±0.01)%, margarate (0.15±0.02)%, and pentadecanoate (0.14±0.01)%. The PCA chemometrics results showed that rat meats had scores that close to cows, which meant they had similar fatty acid composition.
Conclusion: The GC-MS method, combined with PCA chemometrics, tested rat fat with other animals and processed meatballs samples on the market.
2. Fajardo V, Gonzalez Isabel I, Rojas M, Garcia T, Martin R. A review of current PCR-based methodologies for the authentication of meats from game animal species. Trends Food Sci Technol 2010;21:408–21.
3. Che Man YB, Rohman A, Mansor TST. Differentiation of lard from other edible fats and oils by means of Fourier transform infrared spectroscopy and chemometrics. JAOCS, J Am Oil Chem Soc 2011;88:187–92.
4. Lusas EW. Animal and Vegetable Fats, Oils, and Waxes Biotechnology. In: Agriculture and processing human survival is biotechnology; 2015. p. 243-328.
5. Danezis GP, Tsagkaris AS, Camin F, Brusic V, Georgiou CA. Food authentication: techniques, trends and emerging approaches. TrAC-Trends Anal Chem 2016;85:123–32.
6. Pinto A Di, Pinto P Di, Terio V, Bozzo G, Bonerba E, Ceci E. Short communication DNA barcoding for detecting market substitution in salted cod fillets and battered cod chunks. Food Chem 2013;141:1757–62.
7. Von Bargen C, Dojahn J, Waidelich D, Humpf HU, Brockmeyer J. New sensitive high-performance liquid chromatography-tandem mass spectrometry method for the detection of horse and pork in halal beef. J Agric Food Chem 2013;61:11986–94.
8. Kurniasih KSI, Hikmah N, Erwanto Y, Rohman A. Qualitative and quantitative analysis of canine (canis lupus familiaris) meat in meatballs for halal authentication study using real-time polymerase chain reaction. Int J Agric Biol 2020;23:103–8.
9. Tarola AM, Girelli AM, Lorusso S. High performance liquid chromatography determination of fatty acids in drying oils following lipase action. J Chromatogr Sci 2012;50:294–300.
10. Figueiredo IL, Claus T, Oliveira Santos Junior O, Almeida VC, Magon T, Visentainer JV. Fast derivatization of fatty acids in different meat samples for gas chromatography analysis. J Chromatogr A 2016;1456:235–41.
11. Rahayu WS, Martono S, Sudjadi, Rohman A. The potential use of infrared spectroscopy and multivariate analysis for differentiation of beef meatball from dog meat for Halal authentication analysis. J Adv Vet Anim Res 2018;5:307–14.
12. Yuliani F, Riyanto S, Rohman A. Application of ftir spectra combined with chemometrics for analysis of candlenut oil adulteration. Int J Appl Pharm 2018;10:54–9.
13. Nurjuliana M, Che Man YB, Mat Hashim D. Analysis of lard’s aroma by an electronic nose for rapid Halal authentication. JAOCS, J Am Oil Chem Soc 2011;88:75–82.
14. Salamah N, Erwanto Y, Martono S, Maulana I, Rohman A. Differentiation of bovine and porcine gelatines using lc-ms/ms and chemometrics. Int J Appl Pharm 2019;11:159–63.
15. Windarsih A, Rohman A, Swasono RT. Authentication of turmeric using proton-nuclear magnetic resonance spectroscopy and multivariate analysis. Int J Appl Pharm 2018;10:174–80.
16. Indrasti D, Che Man YB, Mustafa S, Hashim DM. Lard detection based on fatty acids profile using comprehensive gas chromatography hyphenated with time-of-flight mass spectrometry. Food Chem 2010;122:1273–7.
17. Hausman GJ, Bergen WG, Etherton TD, Smith SB. The history of adipocyte and adipose tissue research in meat animals. J Anim Sci 2018;96:473–86.
18. Erwanto Y, Abidin MZ, Muslim EYP, Sugiyono S, Rohman A. Identification of pork contamination in meatballs of Indonesia local market using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Asian-Australasian J Anim Sci 2014;27:1487–92.
19. Guntarti A, Prativi SR. Application method of fourier transform infrared (FTIR) combined with chemometrics for analysis of rat meat (Rattus Diardi) in meatballs beef. Pharmaciana 2017;7:133.
20. Guntarti A. Authentication of dog fat with gas chromatography-mass spectroscopy combined with chemometrics. Int J Chem 2018;10:124.
21. Rahmania H, Rohman A. The employment of FTIR spectroscopy in combination with chemometrics for analysis of rat meat in meatball formulation. MESC 2015;100:301–5.
22. Lumakso FA, Riyanto S, Ahmad S, Ahmad S, Salleh A, Mohd F, et al. Application of chemometrics in combination with fourier transform mid infrared spectroscopy for authentication of avocado oil. J Food Pharm Sci 2015;3:12–7.
23. Rohman A, Himawati A, Triyana K, Sismindari, Fatimah S. Identification of pork in beef meatballs using fourier transform infrared spectrophotometry and real-time polymerase chain reaction. Int J Food Prop 2017;20:654–61.
24. Rohman A, Triyana K, Sismindari, Erwanto Y. Differentiation of lard and other animal fats based on triacylglycerols composition and principal component analysis. Int Food Res J 2012;19:475–9.
25. Rahayu WS, Rohman A, Sudjadi, Martono S. Identification of dog for halal autentification with gas chromatography mass spectroscopy (GCMS) and chemometrics. Adv Sci Lett 2018;24:138–41.
26. Guntarti A, Gandjar IG, Jannah NM. Authentication of wistar rat fats with gas chromatography mass spectometry combined by chemometrics. Potravin Slovak J Food Sci 2020;14:52–7.
27. Indriyani L, Rohman A, Riyanto S. Authentication of avocado oil (Persea americana Mill.) using differential scanning calorimetry and multivariate regression. Asian J Agric Res 2016;10:78–86.
28. Johnsen LG, Skou PB, Khakimov B, Bro R. Gas chromatography–mass spectrometry data processing made easy. J Chromatogr A 2017;1503:57–64.
29. Miller J, Miller J. Statistics and chemometrics for analytical chemistry. Sixth edition. Vol. 6th Pearson Education Limited; 2010.
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