PHYTOCHEMICAL ANALYSIS OF CALLUS TWO VARIETIES ORTHOSIPHON ARISTATUS (BLUME) MIQ ON MURASHIGE AND SKOOG MEDIA: A STRATEGIC STEP OF SECONDARY METABOLITE PRODUCTION

  • FAHRAUK FARAMAYUDA School of Pharmacy, Institut Teknologi Bandung (ITB), Bandung, West Java, Indonesia, 40132, Faculty of Pharmacy Universitas Jenderal Achmad Yani (UNJANI), Cimahi, West Java, Indonesia, 40532
  • TOTIK SRI MARIANI School of Life Sciences and Technology, Institut Teknologi Bandung (ITB), Bandung, West Java, Indonesia, 40132
  • ELFAHMI School of Pharmacy, Institut Teknologi Bandung (ITB), Bandung, West Java, Indonesia, 40132, Biosciences and Biotechnology Research Center, Institut Teknologi Bandung (ITB), Bandung, West Java, Indonesia, 40132
  • SUKRASNO School of Pharmacy, Institut Teknologi Bandung (ITB), Bandung, West Java, Indonesia, 40132

Abstract

Objective: The research aimed to provide new information regarding the secondary metabolites content of purple and white-purple Orthosiphon aristatus (Blume) Miq. callus, which can then be used as a basis for developing towards cell suspension and ultimately producing secondary metabolites using bioreactors.


Methods: Callus induction of two varieties of O. aristatus were performed by inoculating sterile leaf explants grown on Murashige and Skoog basal media supplemented with 2,4-dichlorophenoxyacetis acid 0.4 ppm. The secondary metabolites were analysed and quantified using high-performance liquid chromatography with gradient elution.


Results: The results showed the growth of callus two varieties of O. aristatus in growth media MS with 2,4-D 0.4 ppm. Rosmarinic acid content in the acetone extract of the purple variety callus was 1.28% w/w, and the white-purple variety was 2.22% w/w.


Conclusion: This study could form the basis for the development of rosmarinic acid production by In vitro culture modification.

Keywords: Purple variety O. aristatus,, White-purple variety O. aristatus, Modification of in vitro culture, Growth media, Phytochemical profiling of callus

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FARAMAYUDA, F., MARIANI, T. S., ELFAHMI, & SUKRASNO. (2021). PHYTOCHEMICAL ANALYSIS OF CALLUS TWO VARIETIES ORTHOSIPHON ARISTATUS (BLUME) MIQ ON MURASHIGE AND SKOOG MEDIA: A STRATEGIC STEP OF SECONDARY METABOLITE PRODUCTION. International Journal of Applied Pharmaceutics, 13(2), 71-77. https://doi.org/10.22159/ijap.2021.v13s2.14
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