TURMERIC EXTRACT POTENTIAL INHIBIT INFLAMMATORY MARKER IN LPS-STIMULATED MARCOPHAGE CELLS

  • WAHYU WIDOWATI Faculty of Medicine, Maranatha Christian University, Bandung 40163, West Java, Indonesia
  • DIANA KRISANTI JASAPUTRA Faculty of Medicine, Maranatha Christian University, Bandung 40163, West Java, Indonesia
  • KAMILA YASHFA GUNAWAN Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia
  • HANNA SARI WIDYA KUSUMA Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia
  • SEILA ARUMWARDANA Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia
  • CINTANI DEWI WAHYUNI Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia
  • I. NYOMAN EHRICH LISTER Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia
  • CHRISMIS NOVALINDA GINTING Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia
  • ERMI GIRSANG Faculty of Medicine, Universitas Prima Indonesia, Medan 20118, North Sumatera, Indonesia
  • RIZAL RIZAL Aretha Medika Utama, Biomolecular Research Center, Bandung 40163, West Java, Indonesia, Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16426, West Java, Indonesia

Abstract

Objective: Inflammation can be induced by microbiological, chemical, physical factors and plays roles in inflammatory diseases. Turmeric (Curcuma longa L.) has been widely used to provide a diverse array of biological activities, including anti-inflammatory, antimicrobial, also antioxidant. The Turmeric extract (TE) anti-inflammatory potential was conducted using a Lipopolysaccharide (LPS)-induced RAW264.7 macrophage cell line by inhibiting inflammatory mediators especially IL-6, PGE-2, IL-1β, COX-2, TNF-α, iNOS, also NO level.


Methods: The TE safe concentration in LPS-induced macrophage cell line was measured using MTS assay for further assay. The inflammatory markers (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO) were measured using ELISA assay and NO by the nitrate/nitrite colorimetric assay in LPS-induced RAW264.7 cell line. LPS induced inflammatory marker by increasing inflammatory marker (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO).


Results: TE with 100 to 25 µg/ml, caused a significant reduction of cells viability, reaching only 30.27 % live cells. TE with lower concentrations (7.5; 5; 2.5 µg/ml) had no cytotoxic effect on macrophage cells (viability 117.31-131.08 %). LPS induction caused an increase in inflammatory cytokines IL-1β, PGE-2, IL-6, COX-2, TNF-α as well as iNOS and NO. Turmeric extract caused the reduction of the inflammatory cytokines in a dose-dependent manner.


Conclusion: The research resulted that TE has anti-inflammatory activity by decreasing IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, and NO level on LPS-induced RAW264.7 cells.

Keywords: Inflammation, Turmeric, TNFα, IL-6, IL-1β

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WIDOWATI, W., JASAPUTRA, D. K., GUNAWAN, K. Y., KUSUMA, H. S. W., ARUMWARDANA, S., WAHYUNI, C. D., LISTER, I. N. E., GINTING, C. N., GIRSANG, E., & RIZAL, R. (2021). TURMERIC EXTRACT POTENTIAL INHIBIT INFLAMMATORY MARKER IN LPS-STIMULATED MARCOPHAGE CELLS. International Journal of Applied Pharmaceutics, 13(3), 7-11. https://doi.org/10.22159/ijap.2021.v13s3.01
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