OPTIMIZATION OF LACTOBACILLUS PLANTARUM ACTIVITIES IN THE BIOSINTHESIS OF LIPASE ENZYMES
Keywords:Lactobacillus plantarum, Biosynthesis, Lipase, Palm oil, Corn oil
Objective: Lipase was protein compounds that can be used for many human activities. Its main function was to degrade fat including 'wrapping' cholesterol which make easily flowed in the blood. The presence lipase was important because can help the digestive healthy. These enzyme can catalyze a variety of reactions including hydrolysis, alcoholysis, esterification and aminolysis. Lipase was utilized in various sectors, such as fat, oil, milk and pharmaceutical industries. This enzyme biosynthesis can be carried out by Pseudomonas aeruginosa, Lactobacillus plantarum and Aspergillus niger.
Methods: The process through fermentation techniques in lipid containing substrates under optimal conditions required by microorganisms. The fermentation products produced were tested for the presence of lipase enzymes qualitatively and quantitatively. The biosynthesis process can be influence by changes in pH, temperature and the presence of glucose. This study aimed to determine the ability of L. plantarum to produce lipases with vegetables oil substrates. The research used L. plantarum carried out at 37 °C for 24-48 h and pH 6-8 in the vegetable oil substrates.
Results: The fermentation products showed hydrolysis reaction to the test media containing oil lipid with lipase levels of 2.708-3.3000 U/ml
Conclusion: The results showed that Lactobacillus plantarum can synthesize the lipase enzyme in palm oil and corn oil as substrates.
2. Joshi R, Kuila A. Lipase and their different industrial applications: a review. Brazilian J Biol Sci 2018;5:237–47.
3. Priji P, Unni KN, Sajith S, Binod P, Benjamin S. Production, optimization, and partial purification of lipase from Pseudomonas sp. strain BUP6, a novel rumen bacterium characterized from malabari goat. Biotechnol Appl Biochem 2015;62:71–8.
4. Gurung N, Ray S, Bose S, Rai V. A broader view: microbial enzymes and their relevance in industries, medicine, and beyond. Biomed Res Int 2013:1–18. DOI:10.1155/ 2013/329121.
5. Sharma S, Kanwar SS. Organic solvent tolerant lipases and applications. Sci World J 2014:1–15. https://doi.org/ 10.1155/2014/625258.
6. Pramiadi D, Yulianti E, Rakhmawati A. Isolasi dan uji aktivitas enzim lipase termostabil dari bakteri termofilik pasca erupsi. Merapi 2014;3:9–19.
7. Okorie PC, Olasupo N. Growth and extracellular enzyme production by microorganisms isolated from ugba-an indigenous nigerian fermented condiment. Afr J Biotech 2013;12:4158–67.
8. Lanka S, Latha JNL. A short review on various screening methods to isolate potential lipase producers: lipases-the present and future enzymes of biotech industry. Int J Biol Chem 2015;9:207–19.
9. Zheng C. Screening and identification of lipase producing bacterium. IOP Conf Ser Earth Environ Sci 2018;108:1–8.
10. Lanka S, Latha JNL. A short review on various screening methods to isolate potential lipase producers: lipases-the present and future enzymes of biotech industry. Int J Biol Chem 2015;9:207–19.
11. Bestari NC, Suharjono. Uji kualitatif dan kuantitatif isolat bakteri lipolitik dari limbah cair pabrik. J Biotropika 2015;3:151–5.
12. Prasasty VD, Winata V, Hanafi M. Identification and characterization of bacterial lipase from plateu soil in west java. J Kim Terap Indones 2017;18:103–8.
13. Akhmad Hidayatulloh JG, Harlia E. Potensi senyawa metabolit yang dihasilkan. JITP 2019;7:1–6.
14. Wang L, Fan D, Chen W, Terentjev EM. Bacterial growth, detachment and cell size control on polyethylene terephthalate surfaces. Sci Rep 2015;5:1–11.
15. Kumar M, Tiwari SK, Srivastava S. Purification and characterization of enterocin LR/6, a bacteriocin from enterococcus faecium LR/6. Appl Biochem Biotech 2010; 160:40–9.
16. Murni SW, Kholisoh SD, Tanti LD, Petrissia ME. Produksi, karakterisasi, dan isolasi lipase dari aspergillus niger. In: Pengembangan Teknologi Kimia untuk Pengolahan Sumber Daya Alam Indonesia; 2011.
17. Dupont J, White PJ, Carpenter MP, Schaefer EJ, Meydani SN, Elson CE, et al. Food uses and health effects of corn oil. J Am Coll Nutr 1990;9:438–70.
18. Isiaka Adetunji A, Olufolahan Olaniran A. Optimization of culture conditions for enhanced lipase production by an indigenous Bacillus aryabhattai SE3-PB using response surface methodology. Biotechnol Biotechnol Equip 2018;32:1514–26.
19. Hassan SWM, El Latif HHA, Ali SM. Production of cold-active lipase by free and immobilized marine Bacillus cereus HSS: application in wastewater treatment. Front Microbiol 2018;9:1–13.
20. Carrazco Palafox J, Rivera Chavira BE, Ramirez Baca N, Manzanares Papayanopoulos LI, Nevarez Moorillon GV. Improved method for qualitative screening of lipolytic bacterial strains. Methods X 2018:68–74. DOI:10.1016/j.mex. 2018.01.004.
21. Li XL, Zhang WH, Wang YD, Dai YJ, Zhang HT, Wang Y, et al. A high-detergent-performance, cold-adapted lipase from Pseudomonas stutzeri PS59 suitable for detergent formulation. J Mol Catal B Enzym 2014;102:16–24.
22. Mendes DB, Silva FFD, Guarda PM, Almeida AF, Oliveira DPD, Morais PB, et al. Lipolytic enzymes with hydrolytic and esterification activities produced by filamentous fungi isolated from decomposition leaves in an aquatic environment. Enzyme Res 2019;11:1–13.
23. Furini G, Berger JS, Campos JAM, VAN DER SAND ST, Germani JC. Production of lipolytic enzymes by bacteria isolated from biological effluent treatment systems. An Acad Bras Cienc 2018;90:2955–65.