OPTIMIZATION OF LACTOBACILLUS PLANTARUM ACTIVITIES IN THE BIOSINTHESIS OF LIPASE ENZYMES
Objective: Lipase was protein compounds that can be used for many human activities. Its main function was to degrade fat including 'wrapping' cholesterol which make easily flowed in the blood. The presence lipase was important because can help the digestive healthy. These enzyme can catalyze a variety of reactions including hydrolysis, alcoholysis, esterification and aminolysis. Lipase was utilized in various sectors, such as fat, oil, milk and pharmaceutical industries. This enzyme biosynthesis can be carried out by Pseudomonas aeruginosa, Lactobacillus plantarum and Aspergillus niger.
Methods: The process through fermentation techniques in lipid containing substrates under optimal conditions required by microorganisms. The fermentation products produced were tested for the presence of lipase enzymes qualitatively and quantitatively. The biosynthesis process can be influence by changes in pH, temperature and the presence of glucose. This study aimed to determine the ability of L. plantarum to produce lipases with vegetables oil substrates. The research used L. plantarum carried out at 37 °C for 24-48 h and pH 6-8 in the vegetable oil substrates.
Results: The fermentation products showed hydrolysis reaction to the test media containing oil lipid with lipase levels of 2.708-3.3000 U/ml
Conclusion: The results showed that Lactobacillus plantarum can synthesize the lipase enzyme in palm oil and corn oil as substrates.
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