• M. DAVID RAJU Department of Chemistry, PB Siddhartha College of Arts and Science, Vijayawada, A. P.



HPLC, Choline salicylate, Tannic acid, Development, Validation


Objective: The current investigation was pointed at completely unique and reliable high performance liquid chromatographic method for simultaneous quantification of Choline salicylate and Tannic acid.

Methods: Chromatographic separation was achieved on a symmetry C18 column (150x4.6mm, 3.5 µ) using isocratic elution with a buffer containing 1 ml of OPA in l lt of HPLC marked water and acetonitrile within the percentage of 60:40 as movable phase with a flow rate of 1.0 ml/min at ambient temperature. Analysis was achieved within 15 min over an honest linearity within the concentration range from 4-60 µg/ml of choline salicylate and 2.5-37.5 µg/ml of tannic acid. Stress conditions of degradation in acidic, alkaline, peroxide and thermal was studied.

 Results: LOD and LOQ were observed as 1.21 µg/ml, 0.758 µg/ml and 4 µg/ml, 2.5 µg/ml of choline salicylate and tannic acid respectively. Precision and recovery study results were found to be within the suitable limit.

Conclusion: This developed method showed reliable, precise, accurate results under optimized conditions. The method was validated as reported by ICH guidelines. Hence it was evident that the proposed method was suitable for regular analysis and quality control of pharmaceutical preparations.


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