• POTTURI RAMA DEVI Department of Chemistry, RVR and JC College of Engineering, Guntur 522019, A. P., India
  • KANTIPUDI RAMBABU Department of Chemistry, RVR and JC College of Engineering, Guntur 522019, A. P., India




Carboplatin, Docetaxel, RP-HPLC, Development, Validation


Objective: In the current investigation, to separated and validate the cancer healing drugs (Carboplatin and Docetaxel) through the HPLC (e-2695) instrument containing a PDA detector.

Methods: A simple, selective, validated and well-defined stability that shows isocratic RP-HPLC methodology for the quantitative determination of Carboplatin and Docetaxel. The chromatographic strategy utilized Symmetry C18 column of dimensions 150x4.6 mm, 3.5 micron, using isocratic elution with a mobile phase of acetonitrile and 0.1% ortho phosphoric acid (40:60). A flow rate of 1 ml/min and a detector wavelength of 225 nm utilizing the PDA detector were given in the instrumental settings. Recovery, specificity, linearity, accuracy, robustness, ruggedness were determined as a part of method validation and the results were found to be within the acceptable range. Validation of the proposed method was carried out according to an international conference on harmonization (ICH) guidelines.

Results: LOD and LOQ for the two active ingredients were established with respect to test concentration. The calibration charts plotted were linear with a regression coefficient of R2>0.999.

Conclusion: The proposed method to be fast, simple, feasible and affordable in assay condition. During stability tests, it can be used for routine analysis of production samples and to verify the quality of drug samples during stability studies.


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Alfarouk KO, Stock CM, Taylor S, Walsh M, Muddathir AK, Verduzco D, Bashir AH, Mohammed OY, Elhassan GO, Harguindey S, Reshkin SJ, Ibrahim ME, Rauch C. Resistance to cancer chemotherapy: failure in drug response from ADME to P-gp. Cancer Cell Int. 2015;15:71. doi: 10.1186/s12935-015-0221-1, PMID 26180516.

Felici A, Verweij J, Sparreboom A. Dosing strategies for anticancer drugs: the good, the bad and body-surface area. Eur J Cancer. 2002;38(13):1677-84. doi: 10.1016/s0959-8049(02)00151-x, PMID 12175683.

Qato DM, Wilder J, Schumm LP, Gillet V, Alexander GC. Changes in prescription and over-the-counter medication and dietary supplement use among older adults in the united states, 2005 vs 2011. JAMA Intern Med. 2016;176(4):473-82. doi: 10.1001/ jamainternmed.2015.8581, PMID 26998708.

Ebell MH, Culp MB, Radke TJ. A systematic review of symptoms for the diagnosis of ovarian cancer. Am J Prev Med. 2016;50(3):384-94. doi: 10.1016/j.amepre.2015.09.023, PMID 26541098.

Williams C, Simera I, Bryant A. Tamoxifen for relapse of ovarian cancer. Cochrane Database Syst Rev. 2010;2020(3):CD001034. doi: 10.1002/14651858.CD001034.pub2, PMID 20238312.

Oguri S, Sakakibara T, Mase H, Shimizu T, Ishikawa K, Kimura K, Smyth RD. Clinical pharmacokinetics of carboplatin. J Clin Pharmacol. 1988;28(3):208-15. doi: 10.1002/j.1552-4604.1988.tb03134.x, PMID 3283185.

Calvert AH, Harland SJ, Newell DR, Siddik ZH, Jones AC, McElwain TJ, Raju S, Wiltshaw E, Smith IE, Baker JM, Peckham MJ, Harrap KR. Early clinical studies with cis-diammine-1,1-cyclobutane dicarboxylate platinum II. Cancer Chemother Pharmacol. 1982;9(3):140-7. doi: 10.1007/BF00257742, PMID 6761010.

Ramesh D, Vijayakumar BG, Kannan T. Advances in nucleoside and nucleotide analogues in tackling human immunodeficiency virus and hepatitis virus infections. ChemMedChem. 2021;16(9):1403-19. doi: 10.1002/cmdc.202000849. PMID 33427377.

Zaharevitz DW, Anderson LW, Malinowski NM, Hyman R, Strong JM, Cysyk RL. Contribution of de-novo and salvage synthesis to the uracil nucleotide pool in mouse tissues and tumors in vivo. Eur J Biochem. 1992;210(1):293-6. doi: 10.1111/j.1432-1033.1992.tb17420.x, PMID 1446677.

Belloc S, Benkhalifa M, Cohen Bacrie M, Dalleac A, Chahine H, Amar E, Zini A. Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation. J Assist Reprod Genet. 2014;31(5):527-32. doi: 10.1007/s10815-014-0194-3, PMID 24566945.

Speyer BE, Pizzey AR, Ranieri M, Joshi R, Delhanty JD, Serhal P. Fall in implantation rates following ICSI with sperm with high DNA fragmentation. Hum Reprod. 2010;25(7):1609-18. doi: 10.1093/humrep/deq116, PMID 20495207.

Adkins JN, Varnum SM, Auberry KJ, Moore RJ, Angell NH, Smith RD, Springer DL, Pounds JG. Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry. Mol Cell Proteomics. 2002;1(12):947-55. doi: 10.1074/mcp.m200066-mcp200, PMID 12543931.

Kumar E, Kumar A, Parasuraman S, Rajan V, Emerson S. Hepatoprotective activity of clearliv a polyherbal formulation in Wistar rats. Arch Med Health Sci. 2013;1(2):120-5. doi: 10.4103/2321-4848.123023.

Wallace JL. Acetaminophen hepatotoxicity: NO to the rescue. Br J Pharmacol. 2004;143(1):1-2. doi: 10.1038/sj.bjp.0705781, PMID 15345657.

Donadieu J, Beaupain B, Fenneteau O, Bellanne Chantelot C. Congenital neutropenia in the era of genomics: classification, diagnosis, and natural history. Br J Haematol. 2017;179(4):557-74. doi: 10.1111/bjh.14887, PMID 28875503.

Horwitz MS, Corey SJ, Grimes HL, Tidwell T. ELANE mutations in cyclic and severe congenital neutropenia: genetics and pathophysiology. Hematol Oncol Clin North Am. 2013;27(1):19-41. doi: 10.1016/j.hoc.2012.10.004, PMID 23351986.

Jarde T, Perrier S, Vasson MP, Caldefie Chezet F. Molecular mechanisms of leptin and adiponectin in breast cancer. Eur J Cancer. 2011;47(1):33-43. doi: 10.1016/j.ejca.2010.09.005, PMID 20889333.

Jassim GA, Whitford DL, Hickey A, Carter B. Psychological interventions for women with non-metastatic breast cancer. Cochrane Database Syst Rev. 2015;(5):CD008729. doi: 10.1002/14651858.CD008729.pub2, PMID 26017383.

Auperin A. Epidemiology of head and neck cancers: an update. Curr Opin Oncol. 2020;32(3):178-86. doi: 10.1097/CCO.0000000000000629, PMID 32209823.

Vigneswaran N, Williams MD. Epidemiologic trends in head and neck cancer and aids in diagnosis. Oral Maxillofac Surg Clin North Am. 2014;26(2):123-41. doi: 10.1016/j.coms.2014.01.001, PMID 24794262.

Heise K, Bertran E, Andia ME, Ferreccio C. Incidence and survival of stomach cancer in a high-risk population of Chile. World J Gastroenterol. 2009;15(15):1854-62. doi: 10.3748/wjg.15.1854, PMID 19370783.

Bello JO. Predictors of survival outcomes in native sub Saharan black men newly diagnosed with metastatic prostate cancer. BMC Urol. 2017;17(1):39. doi: 10.1186/s12894-017-0228-0, PMID 28558685.

Catalona WJ. Prostate cancer screening. Med Clin North Am. 2018;102(2):199-214. doi: 10.1016/j.mcna.2017.11.001, PMID 29406053.

Simon GR, Turrisi A, American College of Chest Physicians. Management of small cell lung cancer: ACCP evidence-based clinical practice guidelines (2nd edition). Chest. 2nd ed. 2007;132(3);Suppl:324S-39S. doi: 10.1378/chest.07-1385, PMID 17873178.

Sherwood J, Dearden S, Ratcliffe M, Walker J. Mutation status concordance between primary lesions and metastatic sites of advanced non-small-cell lung cancer and the impact of mutation testing methodologies: a literature review. J Exp Clin Cancer Res. 2015;34:92. doi: 10.1186/s13046-015-0207-9, PMID 26338018.

Wills CP, Young M, White DW. Pitfalls in the evaluation of shortness of breath. Emerg Med Clin North Am. 2010;28(1):163-81. doi: 10.1016/j.emc.2009.09.011, PMID 19945605.

Snyder JG, Ambegaonkar JP, Winchester JB, McBride JM, Andre MJ, Nelson AG. Efficacy of cold-water immersion in treating delayed onset muscle soreness in male distance runners. Med Sci Sports Exer. 2011;43(5):766. doi: 10.1249/01.MSS.0000402128.66983.f7.

Blonski W, Siropaides T, Reddy KR. Coagulopathy in liver disease. Curr Treat Options Gastroenterol. 2007;10(6):464-73. doi: 10.1007/s11938-007-0046-7, PMID 18221607.

Tripodi A, Mannucci PM. The coagulopathy of chronic liver disease. N Engl J Med. 2011;365(2):147-56. doi: 10.1056/NEJMra1011170, PMID 21751907.

Kapoor V, Hirst WG, Hentschel C, Preibisch S, Reber S. MTrack: automated detection, tracking, and analysis of dynamic microtubules. Sci Rep. 2019;9(1):3794. doi: 10.1038/s41598-018-37767-1, PMID 30846705.

Ganguly A, Yang H, Sharma R, Patel KD, Cabral F. The role of microtubules and their dynamics in cell migration. J Biol Chem. 2012;287(52):43359-69. doi: 10.1074/jbc.M112.423905, PMID 23135278.

Subrahmanyam Talari, Anuradha AV, Komala Sai Prathyusha Ks A. A validated RP-HPLC method for cisplatin and topotecan in API and vaccine form and its stress studies. Int J Res Pharm Sci. 2021;12:808-14.

Syed Rafi, Kantipudi Rambabu. Stability indicating validated HPLC method for the determination of aceclofenac and misoprostol in bulk and pharmaceutical formulation. IJRPS 2020;11(4):7848-53. doi: 10.26452/ijrps.v11i4.4669.

Bhavani P, Prasada Rao K, Mohan S. Novel validated reversed-phase high-performance liquid chromatography method for determination of glucosamine, diacerein, and methyl sulfonyl methane in micro sample rat plasma and its application to pharmacokinetic and dissolution studies. Asian J Pharm Clin Res. 2020;13:50-63. doi: 10.22159/ajpcr.2020.v13i12.36547.

Shivani CP, Maheshwari DG. Development and validation of UV spectrometric and HPLC method for estimation of escitalopram oxalate and flupentixol dihydrochloride in combined dosage form. AJPTI 2016;4:59-70.

Naresh Kumar DS, Patel D. Stability indicating chromatographic method development and validation for the simultaneous estimation of escitalopram oxalate and flupentixol in its pharmaceutical dosage form by HPLC. WJPR. 2017;6:549-66.

Supriya T, Naresh D, Vijaya Kumar G, Haneer MA. Stability indicating RP-HPLC method development and validation for simultaneous estimation of escitalopram and flupentixol pure and marketed formulation. Asian J Pharm Res. 2018;8:4-10.

Malathi S, Devakumar D. Development and validation of rp-hplc method for the estimation of escitalopram oxalate and flupentixol dihydrochloride in combined dosage form and plasma. Int J Pharm Pharm Sci. 2021;13:61-6.

Mukta D, Naykode MD, Durgacharan A, Bhagwat DA, Swapnil D, Jadhav SD, Harinath N, More HN. Analytical and bioanalytical method for quantification of pure azilsartan, not its salts by RP-HPLC. Research J Pharm and Technol. 2017;10(3):708-14. doi: 10.5958/0974-360X.2017.00133.0.

Mayanka Singh M, Manoj Charde M, Rajesh Shukla R, Rita MC. Determination of calcipotriene in calcipotriene cream 0.05% w/w by RP-HPLC method development and validation. Research J Pharm and Technol. 2011;4:1219-23.

Eluru A, Surendra Babu K. A study of method development, validation and forced degradation for simultaneous quantification of povidone iodine and ornidazole in bulk and pharmaceutical dosage form by using RP-HPLC. IJPSR. 2021;12:1217-22.

Senthil Rajan D, Muruganathan G, Shivkumar K, Ganesh T. Development and validation of HPLC method for simultaneous quantification of vasicine, glycyrrhizin and piperine in poly herbal cough syrup. Int J Curr Pharm Res. 2020;12:15-9.

Malak Y, Al-Bathish AA, Ggazy MK, El-Jamal. Rp-hplc and chemometric methods for the determination of two anti-diabetic mixtures; metformin hydrochloride-canagliflozin and metformin hydrochloride-gliclazide in their pharmaceutical formulation. Int J Pharm Pharm Sci. 2020;12:83-94.

Gadhvi MP, Bhandari A, Suhagia BN, Desai UH. Development and validation of RP-HPLC method for simultaneous estimation of atazanavir and ritonavir in their combined tablet dosage form. Research J Pharm and Technol 2013;6:200-3.

Swati K, Abhishek P, Sushank S, Bothiraja C, Atmaram P. High-performance liquid chromatography for the simultaneous estimation of cefoperazone and sulbactam in rat plasma and its importance in therapeutic drug monitoring. Int J Pharm Pharm Sci. 2020;12:92-7.

Gomathy S, Narenderan ST, Meyyanathan SN, Gowramma B. Development and validation of hplc method for the simultaneous estimation of apigenin and luteolin in commercial formulation. J Crit Rev. 2020;7:4785-90.

Vijayakumari M, Reddy Ch B. Stability indicating validated hplc method for the determination of zanubrutinib in bulk and pharmaceutical dosage form. Asian J Pharm Clin Res. 2020;13:159-62.

Raziq A. Syed Umer Jan. Relative comparison of stability and degradation of methylcobalamine tablets of different brands at different storage settings. Int J Appl Pharm. 2021;13:171-5.

Rajakumari R, Sreenivasa Rao S. Stress degradation studies and development of a validated RP-HPLC method for determination of tiagabine in presence of its degradation products. Int J Pharm Pharm Sci. 2016;8:230-6.

Charu Pandya P, Sadhana Rajput J. Development and validation of stability indicating method RP-HPLC method of aacotiamide. Int J Pharm Pharm Sci. 2018;10:1-8.

Athavia BA, Dedania ZR, Dedania RR, Swamy SMV, Prajapati CB. Stability indicating hplc method for determination of vilazodone hydrochloride. Int J Curr Pharm Sci 2017;9(4). doi: 10.22159/ijcpr.2017v9i4.20975.



How to Cite

DEVI, P. R., & RAMBABU, K. (2022). RAPID DETERMINATION OF CARBOPLATIN AND DOCETAXEL USİNG RP-HPLC WİTH PDA DETECTOR. International Journal of Applied Pharmaceutics, 14(2), 186–192. https://doi.org/10.22159/ijap.2022v14i2.43633



Original Article(s)