5-FLUOROURACIL IMPREGNATED NIOSOMAL-IN SITU GEL (THERMO-SENSITIVE) FOR ORAL CANCER: DESIGN, CHARACTERIZATION, IN VITRO/EX VIVO EVALUATION

Authors

  • RAHUL KUMAR SINGH Department of Pharmaceutics, Rajiv Academy for Pharmacy, P.O. Chhatikara, Mathura-281001, Uttar Pradesh, India
  • ANIRUDH SINGH DEORA Department of Pharmaceutics, Bhupal Nobel’s Institute of Pharmaceutical Sciences, Bhupal Nobel’s University, Central Area, Udaipur – 313001, Rajasthan, India

DOI:

https://doi.org/10.22159/ijap.2022v14i4.44195

Keywords:

5-Fluorouracil, Niosomes, In-Situ Gel, Span-60, Cholesterol, Modified-ether injection method, Modified-cold method.

Abstract

Objective: To formulate and characterize 5-fluorouracil loaded niosomal-in-situ gel for sustained drug delivery to reduce dosing frequency at the same time follow a local drug delivery for cancer targeting.

Methods: Cholesterol and span-60 based niosomes were prepared after following the modified ether injection method. Best formulation selected after characterization through FTIR, SEM, % Entrapment efficiency, zeta potential, polydispersity index, in-vitro release, and vesicle size whereas, based on cold method niosomes encapsulated in-situ gel was formulated and characterized through gelling temperature and time, spreadability and syringeability, gel strength, adhesive force, and drug release.

Results:  Based on various studies included particle size, PDI, zeta potential value, % Entrapment efficiency and % drug release,  F1 formulation was selected as a best formulation, as niosomal particle size of 388.3 nm  proved a higher drug permeation through buccal area, whereas PDI and  zeta potential value of  0.304 and +50.5 are proved a uniform niosomal size with optimum charge distribution  which help to attain higher stability of the formulation, on the other hand % Entrapment efficiency of 87.825% proved that niosomes are capable to hold higher drug concentration, lastly 84.567% of drug release within 12 hrs of time period prove that higher amount of drug release occur by following sustained release pattern. On other hand Mucoadhesion, gelling strength and in-vitro permeation studies prove that niosomes containing in-situ gel has a capacity to adhere over the mucosa with minimum dissolution with saliva up to 12 hrs and is capable of 95% of drug permeation capacity. Lastly FTIR and SEM images confirmed about niosomal formation with optimum stability.

Conclusion: 5-Fluorouracil encapsulated niosomal in-situ gel will be superior and effective alternative to parenteral dosage forms available in the market for mouth cancer treatment.

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Published

07-05-2022

How to Cite

SINGH, R. K., & DEORA, A. S. (2022). 5-FLUOROURACIL IMPREGNATED NIOSOMAL-IN SITU GEL (THERMO-SENSITIVE) FOR ORAL CANCER: DESIGN, CHARACTERIZATION, IN VITRO/EX VIVO EVALUATION. International Journal of Applied Pharmaceutics, 14(4). https://doi.org/10.22159/ijap.2022v14i4.44195

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