DETERMINATION OF AURINTRICARBOXYLIC ACID (A VIRAL INHIBITOR) USING MIXED MICELLAR CLOUD POINT EXTRACTION PROCEDURES

Authors

  • RADHA KRIS DIMMITI Department of Physical and Nuclear Chemistry and Chemical Oceanography, School of Chemistry, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India
  • AMEER KHAN SHAIK Department of Physical and Nuclear Chemistry and Chemical Oceanography, School of Chemistry, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India
  • SHYAMALA PULIPAKA Department of Physical and Nuclear Chemistry and Chemical Oceanography, School of Chemistry, Andhra University, Visakhapatnam 530003, Andhra Pradesh, India

DOI:

https://doi.org/10.22159/ijap.2022v14i4.44206

Keywords:

Cloud point extraction (CPE), Aurintricarboxylic acid (ATCA), Ion-pair, Triton X-114 (TX-114)

Abstract

Objective: Development of micelle mediated cloud point extraction (CPE) and preconcentration of aurintricarboxylic acid (ATCA) (a viral inhibitor).

Methods: The method is based on the ion-pair formation between ATCA and a cationic surfactant tetrabutylammonium bromide (TBAB), which is extracted into the Triton X-114 (TX-114, a non-ionic surfactant) at a pH 4.4. The effect of different important parameters like pH, the concentration of surfactants (TX-114 and TBAB), salt, temperature, and time for the extraction of ATCA was optimized.

Results: Extraction efficiency of 90.28 % for CPE of ATCA was obtained using mixed micelles of TX-114 and TBAB. The linear range and limits of detection for ATCA was found to be 8.44-84.47 µg ml-1 and 8.14 ng ml-1, respectively.

Conclusion: The suggested CPE method has been applied to the determination of ATCA in aqueous solutions.

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References

Bhargavi K, Shyamala P, Rao GN. Determination of protonation constants of viral inhibitor, aurintricarboxylic acid in SDS and CTAB micellar media: A potentiometric study. Asian J Chem. 2021;33(10):2301-5. doi: 10.14233/ajchem.2021.23318.

Ratul B, Ranajit N, Sameer S, Ratna R, Riya B. High-throughput screening and dynamic studies of selected compounds against SARC-COV-2. Int J Appl Pharm. 2022;14:251-60.

Hung HC, Tseng CP, Yang JM, Ju YW, Tseng SN, Chen YF, Chao YS, Hsieh HP, Shih SR, Hsu JTA. Aurintricarboxylic acid inhibits influenza virus neuraminidase. Antiviral Res. 2009;81(2):123-31. doi: 10.1016/j.antiviral.2008.10.006, PMID 19014974.

Walther W, Stein U, Siegel R, Fichtner I, Schlag PM. Use of the nuclease inhibitor aurintricarboxylic acid (ATA) for improved non-viral intratumoral in vivo gene transfer by jet injection. J Gene Med. 2005;7(4):477-85. doi: 10.1002/jgm.690, PMID 15517545.

Kumar R, Chandra A, Gupta S, Gautam PK. Development and validation of UV spectrophotometric method for quantitative estimation of lafutidine in bulk and pharmaceutical dosage form. Int J App Pharm. 2017;9(6):75-9. doi: 10.22159/ijap.2017v9i6.21943.

Ivankovic A. Review of 12 principles of green chemistry in practice. IJSGE. 2017;6(3). doi: 10.11648/j.ijrse.20170603.12.

Kumar KR, Khan SA, Shyamala P. Mixed micellar cloud point extraction and spectrophotometric determination of iron(II) using 1,10-phenanthroline: Effect of sodium nitroprusside on extraction efficiency. Asian J Chem. 2019;31(7):1591-4. doi: 10.14233/ajchem.2019.21999.

Saraswathi TS, Mothilal M. Development and evaluation of rivastigmine loaded self-assembled nanostructures of nonionic surfactants for brain delivery. Int J Appl Pharm. 2021;1:205-15.

Mohammed ZT, Al Ameri AHS, Muntadhar SS. Spectro-photometric determination of ponceau 4R in the several beverages using mixed micelles after cloud point extraction. Journal of Al-Nahrain University 2018;21:8-13.

Wu H, Zhao GY, Du LM. Determination of ofloxacin and gatifloxacin by mixed micelle-mediated cloud point extraction-fluorimetry combined methodology. Spectrochim Acta A Mol Biomol Spectrosc. 2010;75(5):1624-8. doi: 10.1016/j.saa.2010.02.031, PMID 20299280.

Mohammed JMH, Omar QM. Cloud point extraction for the spectrophotometric determination of cefdinir. Al-Mustansiriyah J Sci. 2019;30:85-93.

Ustun Ozgur M, Delmidan M. Development and validation of an effective spectrophotometric method for simultaneous determination of synthetic colorants after cloud point extraction and comparison with new green HPLC method. J AOAC Int. 2019;102(4):1241-52. doi: 10.5740/jaoacint.18-0229, PMID 30646971.

Published

07-07-2022

How to Cite

DIMMITI, R. K., SHAIK, A. K., & PULIPAKA, S. (2022). DETERMINATION OF AURINTRICARBOXYLIC ACID (A VIRAL INHIBITOR) USING MIXED MICELLAR CLOUD POINT EXTRACTION PROCEDURES. International Journal of Applied Pharmaceutics, 14(4), 279–281. https://doi.org/10.22159/ijap.2022v14i4.44206

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Section

Short Communication(s)